UBE2D3
| Gene Symbol | UBE2D3 |
|---|---|
| Entrez Gene | 7323 |
| Alt Symbol | E2(17)KB3, UBC4/5, UBCH5C |
| Species | Human |
| Gene Type | protein-coding |
| Description | ubiquitin-conjugating enzyme E2D 3 |
| Other Description | E2(17)KB 3|PRO2116|ubiquitin carrier protein D3|ubiquitin-conjugating enzyme E2 D3|ubiquitin-conjugating enzyme E2(17)KB 3|ubiquitin-conjugating enzyme E2-17 kDa 3|ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast)|ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5)|ubiquitin-protein ligase D3 |
| Swissprots | A6NM99 Q6IB88 A6NJB1 Q6NXS4 A6NJ93 P61077 Q8N924 |
| Accessions | AAF35234 EAX06138 EAX06139 EAX06140 EAX06141 EAX06142 EAX06143 EAX06144 EAX06145 EAX06146 EAX06147 P61077 AF116686 AAF71106 AI651378 AK095822 BAC04632 AK127304 AK315775 AL050186 AL833414 AU131013 AW675390 BC003395 AAH03395 BC037894 AAH37894 BC066917 AAH66917 BG613555 BM541512 BM750658 BM788287 BM967209 BQ428985 BQ719030 BU567649 BU570661 BU677139 CD242548 CR456916 CAG33197 DA335600 DA494746 DA550074 DA557036 DA588940 DA808523 DA965437 DB234695 DB501483 HY035067 HY109217 U39318 AAA91461 XM_005263200 XP_005263257 XM_005263205 XP_005263262 XM_006714297 XP_006714360 XM_006714299 XP_006714362 XM_006714300 XP_006714363 XM_011532225 XP_011530527 XM_011532226 XP_011530528 XM_011532227 XP_011530529 NM_001300795 NP_001287724 NM_003340 NP_003331 NM_181886 NP_871615 NM_181887 NP_871616 NM_181888 NP_871617 NM_181889 NP_871618 NM_181890 NP_871619 NM_181891 NP_871620 NM_181892 NP_871621 NM_181893 NP_871622 |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA- linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following |
| Subcellular Location | Cell membrane {ECO:0000269|PubMed:18508924}; Peripheral membrane protein {ECO:0000269|PubMed:18508924}. Endosome membrane {ECO:0000269|PubMed:18508924}; Peripheral membrane protein {ECO:0000269|PubMed:18508924}. |
| Top Pathways | Ubiquitin mediated proteolysis, Protein processing in endoplasmic reticulum |
UBE2D3 CRISPR/Cas9 KO Plasmid (h) - sc-405029 from Santa Cruz Biotechnology
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UBE2D3 HDR Plasmid (h) - sc-405029-HDR from Santa Cruz Biotechnology
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UBE2D3 Double Nickase Plasmid (h) - sc-405029-NIC from Santa Cruz Biotechnology
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UBE2D3 Double Nickase Plasmid (h2) - sc-405029-NIC-2 from Santa Cruz Biotechnology
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UBE2D3 CRISPR Activation Plasmid (h) - sc-405029-ACT from Santa Cruz Biotechnology
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UBE2D3 CRISPR Activation Plasmid (h2) - sc-405029-ACT-2 from Santa Cruz Biotechnology
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UBE2D3 Lentiviral Activation Particles (h) - sc-405029-LAC from Santa Cruz Biotechnology
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UBE2D3 Lentiviral Activation Particles (h2) - sc-405029-LAC-2 from Santa Cruz Biotechnology
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UBE2D3 siRNA (h) - sc-41681 from Santa Cruz Biotechnology
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UBE2D3 shRNA Plasmid (h) - sc-41681-SH from Santa Cruz Biotechnology
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UBE2D3 shRNA (h) Lentiviral Particles - sc-41681-V from Santa Cruz Biotechnology
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UbcH5c/UBE2D3 RNAi - H00007323-R09 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R04 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R06 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R03 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R02 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R07 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R08 from Novus Biologicals
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MISSION® esiRNA - EHU063171 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22358 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22358 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09695 from Sigma-Aldrich
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UBE2D3 siRNA (Human) - CRH5006 from Cohesion Biosciences
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UbcH5c/UBE2D3 RNAi - H00007323-R05 from Novus Biologicals
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UbcH5c/UBE2D3 RNAi - H00007323-R01 from Novus Biologicals
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