Gene Symbol | MISP |
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Entrez Gene | 126353 |
Alt Symbol | C19orf21 |
Species | Human |
Gene Type | protein-coding |
Description | mitotic spindle positioning |
Other Description | caprice|mitotic interactor and substrate of PLK1|mitotic interactor and substrate of Plk1|mitotic spindle positioning protein |
Swissprots | Q8IVT2 |
Accessions | EAW61160 Q8IVT2 BC042125 AAH42125 BC052236 AAH52236 BX648389 BX648815 XM_011527685 XP_011525987 XM_011527686 XP_011525988 NM_173481 NP_775752 |
Function | Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytospkeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus. {ECO:0000269|PubMed:23509069, ECO:0000269|PubMed:23574715}. |
Subcellular Location | Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Predominantly localizes to cortical actin structures during interphase and mitosis. Present in retraction fibers, which are formed at former adhesion sites during mitosis, and at spicular membrane protrusions in re- attaching cytokinetic cells. Partially colocalizes with cytoplasmic F-actin. Not detected at microtubules at interphase, nor at spindle during mitosis. |
C19orf21 CRISPR/Cas9 KO Plasmid (h) - sc-409029 from Santa Cruz Biotechnology
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C19orf21 HDR Plasmid (h) - sc-409029-HDR from Santa Cruz Biotechnology
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C19orf21 Double Nickase Plasmid (h) - sc-409029-NIC from Santa Cruz Biotechnology
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C19orf21 Double Nickase Plasmid (h2) - sc-409029-NIC-2 from Santa Cruz Biotechnology
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C19orf21 CRISPR Activation Plasmid (h) - sc-409029-ACT from Santa Cruz Biotechnology
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C19orf21 CRISPR Activation Plasmid (h2) - sc-409029-ACT-2 from Santa Cruz Biotechnology
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C19orf21 Lentiviral Activation Particles (h) - sc-409029-LAC from Santa Cruz Biotechnology
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C19orf21 Lentiviral Activation Particles (h2) - sc-409029-LAC-2 from Santa Cruz Biotechnology
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C19orf21 siRNA (h) - sc-97747 from Santa Cruz Biotechnology
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C19orf21 shRNA Plasmid (h) - sc-97747-SH from Santa Cruz Biotechnology
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C19orf21 shRNA (h) Lentiviral Particles - sc-97747-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU003841 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN4522 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND4522 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06173 from Sigma-Aldrich
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MISP siRNA (Human) - CRJ4859 from Cohesion Biosciences
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shRNA set against Human C19orf21 (NM_173481.2) - SHH250094 from Creative biogene
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Human MISP siRNA - orb275360 from Biorbyt
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MISP shRNA Plasmid (Human) - CHJ4859 from Cohesion Biosciences
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C19ORF21 miRNA 3'UTR clone - MiUTR1H-01193 from Creative biogene
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