SMC3

Gene Symbol SMC3
Entrez Gene 9126
Alt Symbol BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1
Species Human
Gene Type protein-coding
Description structural maintenance of chromosomes 3
Other Description SMC protein 3|basement membrane-associated chondroitin proteoglycan|chondroitin sulfate proteoglycan 6 (bamacan)|chromosome-associated polypeptide|structural maintenance of chromosomes protein 3
Swissprots Q5T482 O60464 A8K156 Q9UQE7
Accessions EAW49557 EAW49558 Q9UQE7 AF020043 AAC14893 AF067163 AAD32447 AJ005015 CAA06289 AK289771 BAF82460 AK315845 BAF98736 BC047324 AAH47324 CA439554 NM_005445 NP_005436
Function Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. {ECO:0000269|PubMed:11076961, ECO:0000269|PubMed:19907496}.
Subcellular Location Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser- 1083 is preferentially associated with unsynapsed chromosomal regions (By similarity). {ECO:0000250}.
Top Pathways Cell cycle, Oocyte meiosis

SMC3 (D47B5) Rabbit mAb - 5696 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D47B5
Western blot analysis of extracts from various cell lines using SMC3 (D47B5) Rabbit mAb.

SMC3 (A-7) - sc-365540 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SMC3 (A-7): sc-365540. Western blot analysis of SMC3 expression in A-673 nuclear extract.

SMC3 (C-16) - sc-8135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMC3 (C-16): sc-8135. Western blot analysis of SMC3 expression in A-431 (A) and A-673 (B) nuclear extracts.

SMC3 (E-3) - sc-376352 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SMC3 (E-3): sc-376352. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.

SMC3 (H-300) - sc-292645 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SMC3 (H-300): sc-292645. Western blot analysis of SMC3 expression in A-673 (A) and Hep G2 (B) nuclear extracts.

SMC3 (R-19) - sc-8198 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMC3 (R-19): sc-8198. Western blot analysis of SMC3 expression in A-673 nuclear extract.

Anti-SMC3 antibody - ab89618 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-SMC3 antibody (ab89618) at 1 µg/ml + human colon tissue lysate at 50 µg

Anti-SMC3 antibody [EPR7984] - ab128919 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7984
All lanes : Anti-SMC3 antibody [EPR7984] (ab128919) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : A431 cell lysateLane 3 : K562 cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-SMC3 antibody [EPR7984] (Alexa Fluor® 647) - ab201356 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7984
ab201356 staining SMC3 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201356 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min)

Anti-SMC3 / HCAP Antibody (aa994-1181, Biotin) - LS-C298786 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMC3 / HCAP Antibody (aa994-1181, FITC) - LS-C302391 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMC3 / HCAP Antibody (aa401-431) - LS-C330163 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMC3 / HCAP Antibody (aa215-264) - LS-C116339 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMC3 antibody LS-C116339 Western blot of COLO205 cell lysate.

Anti-SMC3 / HCAP Antibody (aa402-430) - LS-C161312 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SMC3 Antibody western blot of CEM cell line lysates (35 ug/lane). The SMC3 antibody detected the SMC3 protein (arrow).

Anti-SMC3 / HCAP Antibody (aa325-375) - LS-C288823 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse SMC3 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibody: Affinity purified rabbit anti-SMC3 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-SMC3 / HCAP Antibody (C-Terminus, clone EPR7984) - LS-C152546 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7984
IHC of paraffin-embedded human colon tissue using anti-SMC3 antibody.

Anti-SMC3 / HCAP Antibody (aa994-1181) - LS-C296522 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant SMC3 / HCAP.

Anti-SMC3 / HCAP Antibody (clone 2F11) - LS-C139467 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2F11
Western blot of SMC3 expression in transfected 293T cell line by CSPG6 monoclonal antibody (M02), clone 2F11.

Anti-SMC3 / HCAP Antibody - LS-C332006 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HeLa cell line, using SMC3 antibody.

Anti-SMC3 / HCAP Antibody (C-Terminus) - LS-C286329 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse SMC3 by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at a dilution of 1:250. Detection: DAB.

Anti-SMC3 / HCAP Antibody (phospho-Ser1083) - LS-C287367 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse Phospho-SMC3 (S1083) by Immunohistochemistry. Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-phospho SMC3 (S1083) used at a dilution of 1:2,500 (0.4 ug/ml). Detection: DAB.

Anti-SMC3 / HCAP Antibody (phospho-Ser1083) - LS-C286341 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse Phospho-SMC3 (S1083) by Immunohistochemistry. Samples: FFPE serial sections of mouse squamous cell carcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho-SMC3. Antibody: Affinity purified rabbit anti-Phospho-SMC3 (S1083) used at a dilution of 1:250. Detection: DAB staining using anti-rabbit IHC Antibody at a dilution of 1:100.

Anti-SMC3 / HCAP Antibody (C-Terminus) - LS-C287002 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SMC3 by Immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-SMC3 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Anti-SMC3 / HCAP Antibody (aa1051-1100) IHC-plus™ - LS-B5803 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SMC3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5803 concentration 5 ug/ml.

Anti-SMC3 / HCAP Antibody - LS-C91819 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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