| Gene Symbol | CHCHD4 |
|---|---|
| Entrez Gene | 131474 |
| Alt Symbol | MIA40, TIMM40 |
| Species | Human |
| Gene Type | protein-coding |
| Description | coiled-coil-helix-coiled-coil-helix domain containing 4 |
| Other Description | coiled-coil-helix-coiled-coil-helix domain-containing protein 4|mitochondrial intermembrane space import and assembly 40 homolog|mitochondrial intermembrane space import and assembly protein 40|translocase of inner mitochondrial membrane 40 homolog |
| Swissprots | Q96MY6 Q96AI2 Q8N4Q1 A8K3Z9 |
| Accessions | EAW64180 EAW64181 EAW64182 EAW64183 Q8N4Q1 AK056271 BAB71132 AK290764 BAF83453 BC017082 AAH17082 BC033775 AAH33775 DB452249 DB501565 NM_001098502 NP_001091972 NM_144636 NP_653237 |
| Function | Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system. {ECO:0000269|PubMed:16185709, ECO:0000269|PubMed:19182799, ECO:0000269|PubMed:21059946, ECO:0000269|PubMed:23186364}. |
| Subcellular Location | Mitochondrion intermembrane space {ECO:0000269|PubMed:16185709, ECO:0000269|PubMed:23186364}. |
| Tissue Specificity | Expressed in all tissues tested, suggesting an ubiquitous expression. {ECO:0000269|PubMed:16185709}. |
CHCHD4 CRISPR/Cas9 KO Plasmid (h) - sc-417290 from Santa Cruz Biotechnology
|
||||||||
CHCHD4 HDR Plasmid (h) - sc-417290-HDR from Santa Cruz Biotechnology
|
||||||||
CHCHD4 Double Nickase Plasmid (h) - sc-417290-NIC from Santa Cruz Biotechnology
|
||||||||
CHCHD4 Double Nickase Plasmid (h2) - sc-417290-NIC-2 from Santa Cruz Biotechnology
|
||||||||
CHCHD4 CRISPR Activation Plasmid (h) - sc-417290-ACT from Santa Cruz Biotechnology
|
||||||||
CHCHD4 CRISPR Activation Plasmid (h2) - sc-417290-ACT-2 from Santa Cruz Biotechnology
|
||||||||
CHCHD4 Lentiviral Activation Particles (h) - sc-417290-LAC from Santa Cruz Biotechnology
|
||||||||
CHCHD4 Lentiviral Activation Particles (h2) - sc-417290-LAC-2 from Santa Cruz Biotechnology
|
||||||||
CHCHD4 siRNA (h) - sc-78156 from Santa Cruz Biotechnology
|
||||||||
CHCHD4 shRNA Plasmid (h) - sc-78156-SH from Santa Cruz Biotechnology
|
||||||||
CHCHD4 shRNA (h) Lentiviral Particles - sc-78156-V from Santa Cruz Biotechnology
|
||||||||
MISSION® esiRNA - EHU111221 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN6035 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6035 from Sigma-Aldrich
|
||||||||
MISSION® 3′UTR Lenti GoClone™ - HUTR07068 from Sigma-Aldrich
|
||||||||
CHCHD4 siRNA (Human) - CRJ5065 from Cohesion Biosciences
|
||||||||
CHCHD4 - MBS8217464 from MyBioSource
|
||||||||
shRNA set against Human CHCHD4 (NM_144636.2) - SHH262761 from Creative biogene
|
||||||||
shRNA set against Human CHCHD4(NM_144636.2) - SHG173845 from Creative biogene
|
||||||||
Human CHCHD4 siRNA - orb275566 from Biorbyt
|
||||||||
CHCHD4 shRNA Plasmid (Human) - CHJ5065 from Cohesion Biosciences
|
||||||||
CHCHD4 miRNA 3'UTR clone - MiUTR1H-02095 from Creative biogene
|
||||||||
CHCHD4 miRNA 3'UTR clone - MiUTR3H-11639 from Creative biogene
|