Gene Symbol | Eed |
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Entrez Gene | 13626 |
Alt Symbol | ENSMUSG00000039373, l(7)5Rn, l7Rn5, lusk |
Species | Mouse |
Gene Type | protein-coding |
Description | embryonic ectoderm development |
Other Description | lethal, Chr 7, Rinchik 5|polycomb protein EED |
Swissprots | P97462 Q921E6 |
Accessions | EDL06770 EDL06771 Q921E6 AK016228 BAB30159 AK053947 AK077664 BAC36938 AK087924 AK131976 BAE20916 AK133890 BAE21915 AK213110 BC012966 AAH12966 BQ899891 CJ200028 U78103 AAB38319 U97675 AAC53302 XM_006507341 XP_006507404 XR_869658 XR_869659 NM_021876 NP_068676 |
Function | Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptio |
Subcellular Location | Nucleus. Chromosome. Note=Localizes to the inactive X chromosome in cells of the early embryo and in stem cells of the extraembryonic trophectoderm lineage. Recruitment to the inactive X-chromosome requires XIST. |
Tissue Specificity | Expressed in brain, heart, kidney, liver, lung, muscle, ovary, spleen and testis. Expressed throughout the brain. {ECO:0000269|PubMed:17259173, ECO:0000269|PubMed:8805699, ECO:0000269|PubMed:9234727}. |
EED CRISPR/Cas9 KO Plasmid (m) - sc-420114 from Santa Cruz Biotechnology
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EED HDR Plasmid (m) - sc-420114-HDR from Santa Cruz Biotechnology
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EED Double Nickase Plasmid (m) - sc-420114-NIC from Santa Cruz Biotechnology
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EED Double Nickase Plasmid (m2) - sc-420114-NIC-2 from Santa Cruz Biotechnology
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EED CRISPR Activation Plasmid (m) - sc-420114-ACT from Santa Cruz Biotechnology
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EED CRISPR Activation Plasmid (m2) - sc-420114-ACT-2 from Santa Cruz Biotechnology
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EED Lentiviral Activation Particles (m) - sc-420114-LAC from Santa Cruz Biotechnology
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EED Lentiviral Activation Particles (m2) - sc-420114-LAC-2 from Santa Cruz Biotechnology
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EED siRNA (m) - sc-37824 from Santa Cruz Biotechnology
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EEDt shRNA Plasmid (m) - sc-37824-SH from Santa Cruz Biotechnology
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EEDt shRNA (m) Lentiviral Particles - sc-37824-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU178331 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN30440 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND30440 from Sigma-Aldrich
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EED siRNA (Mouse) - CRM1138 from Cohesion Biosciences
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EED - MBS8230807 from MyBioSource
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shRNA set against Mouse EED (NM_021876.3) - SHH283249 from Creative biogene
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Mouse EED siRNA - orb280614 from Biorbyt
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EED shRNA Plasmid (Mouse) - CHM1138 from Cohesion Biosciences
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