USP9X

Gene Symbol USP9X
Entrez Gene 8239
Alt Symbol DFFRX, FAF, FAM, MRX99
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 9, X-linked
Other Description Drosophila fat facets related, X-linked|deubiquitinating enzyme FAF-X|fat facets in mammals|fat facets protein related, X-linked|fat facets protein-related, X-linked|hFAM|probable ubiquitin carboxyl-terminal hydrolase FAF-X|ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila)|ubiquitin thioesterase FAF-X|ubiquitin thiolesterase FAF-X|ubiquitin-specific processing protease FAF-X|ubiquitin-specific protease 9, X chromosome|ubiquitin-specific-processing protease FAF-X
Swissprots Q8WWT3 Q8WX12 O75550 Q93008
Accessions EAW59406 EAW59407 EAW59408 EAW59409 EAW59410 EAW59411 EAW59412 Q93008 AA261797 AB209666 BAD92903 AF070645 AAC25395 AJ012078 AK294828 BAG57940 AW183065 BC014792 BC046205 AAH46205 BC054861 BC063645 AAH63645 BC172429 AAI72429 BE925861 BX956755 CD619095 DA854185 HG512500 X98296 CAA66942 XM_005272675 XP_005272732 XM_005272676 XP_005272733 NM_001039590 NP_001034679 NM_001039591 NP_001034680 NM_004652 NM_021906
Function Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Essential component of TGF-beta/BMP signaling cascade. Regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. Specifically hydrolyzes both 'Lys-29'- and 'Lys-33'-linked polyubiquitins chains. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33. Involved in axonal growth and neuronal cell migration. {ECO:0000269|PubMed:16322459, ECO:0000269|PubMed:18254724, ECO:0000269|PubMed:19135894, ECO:0000269|PubMed:24607389}.
Subcellular Location Cytoplasm. Cell projection, growth cone.
Tissue Specificity Widely expressed in embryonic and adult tissues.

Human USP9x peptide - ab20617 from Abcam

Category Peptide
All lanes : Anti-USP9x antibody ( ab19879 ) at 1 µg/ml Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate ( ab3950 ) Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate ( ab3950 ) with Human USP9x peptide (ab20617) at 1 µg/ml Lysates/proteins at 20 µg per lane. Secondary Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 54, 100,105 , 290 kDa Observed band size : 100,105,290 kDa ( why is the actual band size different from the predicted? ) Additional bands at : ~50-65 kDa. We are unsure as to the identity of these extra bands.

Human USP9x peptide - ab20618 from Abcam

Category Peptide

Human USP9x protein fragment - ab152844 from Abcam

Category Protein
Source Wheat germ
Species Human
12.5% SDS-PAGE analysis of ab152844 stained with Coomassie Blue.

Recombinant Human His6-USP9x Isoform 2, CF - ab188692 from Abcam

Source Baculovirus infected Sf21 cells
Species Human

USP9X / FAM Antibody Blocking Peptide - LS-E21707 from LifeSpan Bioscience

Category Peptide
Prices $145.00
Sizes 100 µg

USP9X / FAM Antibody Blocking Peptide - LS-E26770 from LifeSpan Bioscience

Category Peptide
Prices $145.00
Sizes 100 µg

Recombinant Human USP9x Protein - H00008239-Q01 from Novus Biologicals

Category Protein
Prices $369.00, $529.00
Sizes 10 µg, 25 µg
Source Wheat Germ
Species Human

Recombinant Human His6-USP9x Isoform 2, CF - E-552-025 from R&D Systems

Category Protein
Source Spodoptera frugiperda, Sf 21 (baculovirus)-derived, Contains an N-terminal Met and 6-His tag

USP9X - H00008239-Q01-10 from Acris Antibodies

Category Protein
Prices $380.00
Sizes 10 µg
Source in vitro transl.
Species Human

USP9X - H00008239-Q01-25 from Acris Antibodies

Category Protein
Prices $500.00
Sizes 25 µg
Source in vitro transl.
Species Human

Recombinant Human USP9X, GST-tagged - USP9X-184H from Creative Biomart

Category Protein
Source E. coli
Species Human

Recombinant Human USP9X, Met&His-tagged - USP9X-658H from Creative Biomart

Category Protein
Source Baculovirus Sf 21 Insect cells
Species Human

Recombinant Human Probable ubiquitin carboxyl-terminal hydrolase FAF-X(USP9X) ,partial - CSB-YP856610HU CSB-EP856610HU CSB-BP856610HU CSB-MP856610HU from Cusabio

Category Protein
Source Yeast or E.coli or Baculovirus or Mammalian cell
Species Human

Human USP9x full length protein - ab205998 from Abcam

Category Protein
Source E. coli
Species Human
SDS-PAGE analysis of ab205998 (5µg).

Recombinant Human His6-USP9x Isoform 2 Protein, CF - E-552 from R&D Systems

Category Protein
Prices $295.00
Sizes 25 µg
Source Spodoptera frugiperda , Sf 21 (baculovirus)-derived Contains an N-terminal Met and 6-His tag
Species Human

Probable ubiquitin carboxyl-terminal hydrolase FAF-X (USP9X), partial, Recombinant Protein - MBS1321678 from MyBioSource

Category Protein
Source E Coli or Yeast or Baculovirus or Mammalian Cell
Species Human

USP9X Peptide, Human - M34GP17202H from Creative Peptides

Category Peptide
Source Synthetic.
Species Human

Recombinant Human His6-USP9x Isoform 2 Protein, CF - E-552 from Novus Biologicals

Category Protein
Prices $295.00
Sizes 25 µg
Species Human
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