Gene Symbol | USP30 |
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Entrez Gene | 84749 |
Alt Symbol | - |
Species | Human |
Gene Type | protein-coding |
Description | ubiquitin specific peptidase 30 |
Other Description | deubiquitinating enzyme 30|ub-specific protease 30|ubiquitin carboxyl-terminal hydrolase 30|ubiquitin specific protease 30|ubiquitin thioesterase 30|ubiquitin thiolesterase 30|ubiquitin-specific protease 30|ubiquitin-specific-processing protease 30 |
Swissprots | Q8WTU7 Q9BSS3 Q70CQ3 Q96JX4 |
Accessions | CAE89969 EAW97842 EAW97843 EAW97844 Q70CQ3 AA856774 AI867665 AJ586136 CAE51936 AK027820 BAB55392 AK097830 BAG53537 AL834278 BC004868 AAH04868 BC022094 AAH22094 BG149730 BX095894 CN367077 DQ893401 ABM84327 DQ896783 ABM87782 HY127211 XM_005253962 XP_005254019 XM_005253963 XP_005254020 XM_005253965 XP_005254022 XM_006719653 XP_006719716 XM_011538894 XP_011537196 NM_001301175 NP_001288104 NM_032663 NP_116052 |
Function | Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy (PubMed:18287522, PubMed:24896179, PubMed:25527291, PubMed:25621951). Preferentially cleaves 'Lys-6'- and 'Lys-11'- linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling (PubMed:25621951). Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (PubMed:25527291). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity). {ECO:0000250|UniProtKB:Q3UN04, ECO:0000269|PubMed:18287522, ECO:0000269|PubMed:24896179, ECO:0000269|PubMed:25527291, ECO:0000269|PubMed:25621951}. |
Subcellular Location | Mitochondrion outer membrane {ECO:0000269|PubMed:18287522, ECO:0000269|PubMed:24896179}. |
Tissue Specificity | Expressed in skeletal muscle, pancreas, liver and kidney. {ECO:0000269|PubMed:14715245}. |
USP30 CRISPR/Cas9 KO Plasmid (h) - sc-407851 from Santa Cruz Biotechnology
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USP30 HDR Plasmid (h) - sc-407851-HDR from Santa Cruz Biotechnology
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USP30 Double Nickase Plasmid (h) - sc-407851-NIC from Santa Cruz Biotechnology
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USP30 Double Nickase Plasmid (h2) - sc-407851-NIC-2 from Santa Cruz Biotechnology
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USP30 CRISPR Activation Plasmid (h) - sc-407851-ACT from Santa Cruz Biotechnology
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USP30 CRISPR Activation Plasmid (h2) - sc-407851-ACT-2 from Santa Cruz Biotechnology
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USP30 Lentiviral Activation Particles (h) - sc-407851-LAC from Santa Cruz Biotechnology
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USP30 Lentiviral Activation Particles (h2) - sc-407851-LAC-2 from Santa Cruz Biotechnology
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USP30 siRNA (h) - sc-96007 from Santa Cruz Biotechnology
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USP30 shRNA Plasmid (h) - sc-96007-SH from Santa Cruz Biotechnology
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USP30 shRNA (h) Lentiviral Particles - sc-96007-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU155291 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22579 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22579 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR03813 from Sigma-Aldrich
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USP30 siRNA (Human) - CRJ3609 from Cohesion Biosciences
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USP30 - MBS8200582 from MyBioSource
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shRNA set against Human USP30 (NM_032663.3) - SHH441468 from Creative biogene
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shRNA set against Human USP30(NM_032663.3) - SHL117390 from Creative biogene
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Human USP30 siRNA - orb274110 from Biorbyt
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USP30 shRNA Plasmid (Human) - CHJ3609 from Cohesion Biosciences
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USP30 miRNA 3'UTR clone - MiUTR1H-11058 from Creative biogene
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