PNP

Gene Symbol PNP
Entrez Gene 4860
Alt Symbol NP, PRO1837, PUNP
Species Human
Gene Type protein-coding
Description purine nucleoside phosphorylase
Other Description HEL-S-156an|epididymis secretory sperm binding protein Li 156an|inosine phosphorylase|inosine-guanosine phosphorylase|purine-nucleoside:orthophosphate ribosyltransferase
Swissprots Q15160 B2R8S5 P00491 D3DS00 Q5PZ03
Accessions AAA36460 AAV68044 EAW66458 EAW66459 P00491 AK098544 BAC05327 AK126154 AK307340 AK307364 AK313490 BAG36272 BC104206 AAI04207 BC104207 AAI04208 BC106074 AAI06075 BM193354 BU622706 CR407607 CAG28535 EU794649 ACJ13703 X00737 CAA25320 NM_000270 NP_000261
Function The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta- (deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. {ECO:0000269|PubMed:2104852}.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000250}. Cytoplasm {ECO:0000269|PubMed:22509282}.
Tissue Specificity Expressed in red blood cells; overexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology. {ECO:0000269|PubMed:22509282}.
Top Pathways Purine metabolism, Nicotinate and nicotinamide metabolism, Pyrimidine metabolism

PNP (F-10) - sc-365551 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNP (F-10): sc-365551. Western blot analysis of PNP expression in non-transfected: sc-117752 (A) and human PNP transfected: sc-111683 (B) 293T whole cell lysates.

PNP (H-7) - sc-365081 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNP (H-7): sc-365081. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

PNP (H-56) - sc-135163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PNP (H-56): sc-135163. Western blot analysis of PNP expression in non-transfected: sc-117752 (A) and human PNP transfected: sc-111683 (B) 293T whole cell lysates.

PNP (D-11) - sc-271890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNP (D-11): sc-271890. Western blot analysis of PNP expression in non-transfected: sc-117752 (A) and human PNP transfected: sc-111683 (B) 293T whole cell lysates.

PNP (K-12) - sc-47335 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNP (K-12): sc-47335. Western blot analysis of PNP expression in non-transfected: sc-117752 (A) and human PNP transfected: sc-111683 (B) 293T whole cell lysates.

PNP (E-12) - sc-47334 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNP (E-12): sc-47334. Western blot analysis of PNP expression in mouse spleen tissue extract.

PNP (C-14) - sc-47333 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNP (C-14): sc-47333. Western blot analysis of PNP expression in non-transfected: sc-117752 (A) and human PNP transfected: sc-111683 (B) 293T whole cell lysates.

PNP (N-12) - sc-47336 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PNP (D-8) - sc-365132 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNP (D-8): sc-365132. Western blot analysis of PNP expression in non-transfected: sc-117752 (A) and human PNP transfected: sc-111683 (B) 293T whole cell lysates.

Anti-Nucleoside phosphorylase antibody - ab87789 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-Nucleoside phosphorylase antibody (ab87789) at 1 µg/ml (in 5% skim milk / PBS buffer) + THP-1 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Nucleoside phosphorylase antibody - ab174763 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-Nucleoside phosphorylase antibody (ab174763) at 0.1 µg/ml + Human liver lysate at 35 µgdeveloped using the ECL technique

Anti-Nucleoside phosphorylase antibody - ab154257 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Nucleoside phosphorylase with ab154257 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

Anti-Nucleoside phosphorylase antibody [11] - ab80225 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 11
Anti-Nucleoside phosphorylase antibody [11] (ab80225) at 1/1000 dilution + recombinant NP protein

Anti-Nucleoside phosphorylase antibody [EPR5714] - ab109559 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5714
All lanes : Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : JAR cell lysateLane 3 : 293T cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.

Anti-Nucleoside phosphorylase antibody [EPR5715] - ab109447 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5715
All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilutionLane 1 : Jurkat lysateLane 2 : JAR lysateLane 3 : K562 lysateLane 4 : Human placenta lysateLane 5 : 293T lysateLysates/proteins at 10 µg per lane.

Anti-Nucleoside phosphorylase antibody - C-terminal - ab186046 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Nucleoside phosphorylase by Western Blot of Immunprecipitate.Lane 1: ab186046 at 1 µg/ml labeling Nucleoside phosphorylase in 293T whole cell lysate immunoprecipitated using ab186046 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).Lane 2: Control IgG.Detection: Chemiluminescence with exposure time of 30 seconds. 

Anti-NP (4-Hydroxy-3-Nitro-Phenylacetyl) Antibody - LS-C131096 from LifeSpan Bioscience

Prices $590.00
Sizes 2 ml
Host Human
Clonality Monoclonal

Anti-NP (4-Hydroxy-3-Nitro-Phenylacetyl) Antibody - LS-C58426 from LifeSpan Bioscience

Prices $445.00
Sizes 500 µg
Host Human
Clonality Polyclonal

Anti-NP (4-Hydroxy-3-Nitro-Phenylacetyl) Antibody (clone JW393A) - LS-C58459 from LifeSpan Bioscience

Prices $445.00
Sizes 500 µg
Host Human
Clonality Monoclonal
Clone JW393A

Anti-NP (4-Hydroxy-3-Nitro-Phenylacetyl) Antibody (Internal) - LS-C145152 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PNP / Nucleoside Phosphorylase Antibody (clone 6E5) - LS-C197795 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6E5
Western Blot analysis of NP expression in transfected 293T cell line by NP monoclonal antibody (M01), clone 6E5.Lane 1: NP transfected lysate (Predicted MW: 32.1 KDa).Lane 2: Non-transfected lysate.

Anti-PNP / Nucleoside Phosphorylase Antibody (aa239-289) - LS-C290881 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PNP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-PNP antibody used for IP at 6 ug/mg lysate. PNP was inefficiently immunoprecipitated by rabbit anti-PNP antibody BL15626. For blotting immunoprecipitated PNP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PNP / Nucleoside Phosphorylase Antibody (aa145-178) - LS-C344355 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysates from Daudi, HT-1080, K562, MCF-7, MDA-MB-231 cell line (from left to right) with PNP Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Anti-PNP / Nucleoside Phosphorylase Antibody (aa68-289) - LS-C137543 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-PNP / Nucleoside Phosphorylase Antibody (aa142-153) IHC-plus™ - LS-B10515 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-Nucleoside Phosphorylase / PNP antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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