MED22

Gene Symbol	MED22
Entrez Gene	6837
Alt Symbol	MED24, SURF5, surf-5
Species	Human
Gene Type	protein-coding
Description	mediator complex subunit 22
Other Description	mediator of RNA polymerase II transcription subunit 22\|surfeit locus protein 5
Swissprots	O76072 B3KW83 B3KWX4 Q15528 Q5T8U0
Accessions	CAA12052 CAA12053 CAA76691 CAI12827 CAI12829 CAI12830 CAI12831 EAW88056 EAW88057 EAW88058 EAW88059 EAW88060 Q15528 AJ224358 CAA11915 AJ224359 CAA11916 AJ224360 CAA11917 AK124518 BAG54045 AK126069 BAG54286 BC024225 AAH24225 BC040111 AAH40111 BI756582 GD160955 HQ448554 ADQ33033 HY154869 X85178 CAA59462 NM_133640 NP_598395 NM_181491 NP_852468 NM_006752
Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Subcellular Location	Nucleus {ECO:0000305}.

Med22 (D-10) - sc-393738 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

Med22 (D-10): sc-393738. Western blot analysis of Med22 expression in HL-60 (A) and HeLa (B) whole cell lysates.

Med22 (D-13) - sc-107737 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Med22 (H-132) - sc-368557 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Med22 (H-132): sc-368557. Western blot analysis of Med22 expression in non-transfected: sc-117752 (A) and human Med22 transfected: sc-371730 (B) 293T whole cell lysates.

Med22 (T-15) - sc-107739 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-SURF5 antibody - ab113002 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-SURF5 antibody (ab113002) at 1/100 dilution + HL60 cell line lysate at 35 µg

Anti-SURF5 antibody - ab172205 from Abcam

Prices	$370.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

All lanes : Anti-SURF5 antibody (ab172205) at 1/500 dilutionLane 1 : SURF5 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-MED22 Antibody (clone 3A9) - LS-C198290 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3A9

Anti-MED22 Antibody (aa1-30, AP) - LS-C215766 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa1-30, Biotin) - LS-C215768 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa1-30, APC) - LS-C215767 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa1-30, FITC) - LS-C215769 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa1-30, Azide-free, HRP) - LS-C215770 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa1-30, PE) - LS-C215771 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa1-30) - LS-C164118 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

MED22 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The MED22 antibody detected the MED22 protein (arrow).

Anti-MED22 Antibody (aa93-142) - LS-C152176 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody (aa7-36) - LS-C322939 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-MED22 Antibody - LS-C340990 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

MED24 Antibody - NBP1-85444 from Novus Biologicals

Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: MED24 Antibody [NBP1-85444] - Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoskeleton (microtubules).

SURF5 Antibody - NBP1-87818 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: SURF5 Antibody [NBP1-87818] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

SURF5 Antibody - H00006837-B01 from Novus Biologicals

Prices	$349.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Western Blot: SURF5 Antibody [H00006837-B01] - analysis of SURF5 expression in transfected 293T cell line by SURF5 MaxPab polyclonal antibody. Lane 1: SURF5 transfected lysate (15.4 KDa). Lane 2: Non-transfected lysate.

SURF5 Antibody (3A9) - H00006837-M01 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3A9

Monoclonal Anti-SURF5, (N-terminal) antibody produced in mouse - SAB1402934 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	3A9

<B>Immunoblotting</B><BR/>Monoclonal Anti-SURF5: <B>Cat. No. SAB1402934</B>: Antibody concentration: 1 μg/mL. Specific band of ~37.1 kDa using immunogen protein lysate.

ANTI-MED22(N-TERMINAL) antibody produced in rabbit - SAB1306789 from Sigma-Aldrich

Prices	$326.00
Sizes	40 tst
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-MED22 (N-term): <B>Cat. No. SAB1306789</B>: MED22 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35 μg/lane).

Anti-MED22 antibody produced in rabbit - HPA020391 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-MED22: <B>Cat. No. HPA020391</B>: Immunohistochemical staining of human kidney shows strong membranous positivity in renal glomeruli.

MED22 Antibody - middle region (ARP66395_P050) - ARP66395_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

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