RFNG

Gene Symbol	RFNG
Entrez Gene	5986
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	RFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Other Description	O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase\|beta-1,3-N-acetylglucosaminyltransferase radical fringe\|radical fringe homolog
Swissprots	Q9Y644 O00588
Accessions	AAD34321 EAW89735 EAW89736 EAW89737 EAW89738 EAW89739 EAW89740 Q9Y644 AF038196 AK094016 BAC04269 AK293568 BAG57039 AK302068 BAG63458 BC014495 BC035781 BC050399 BC069034 BC080181 BC146805 AAI46806 BF435018 U94353 AAC51359 XM_011523587 XP_011521889 NM_002917 NP_002908
Function	Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. May be involved in limb formation and in neurogenesis (By similarity). {ECO:0000250}.
Subcellular Location	Golgi apparatus membrane {ECO:0000305}; Single-pass type II membrane protein {ECO:0000305}.
Top Pathways	Other types of O-glycan biosynthesis, Notch signaling pathway

Radical Fringe (C-14) - sc-324261 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Radical Fringe (S-16) - sc-324260 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Radical Fringe (18-K2) - sc-100754 from Santa Cruz Biotechnology

Prices	$295.00
Sizes	100 µg/ml
Host	Mouse
Clonality	Monoclonal

Radical Fringe (18-K2): sc-100754. Western blot analysis of Radical Fringe expression in Hep G2 whole cell lysate.

Anti-Radical Fringe antibody - ab42429 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Radical Fringe antibody (ab42429) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HEK293 Human embryonic kidney cell line Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.

Anti-Radical Fringe antibody - ab129552 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Radical Fringe antibody (ab129552) at 1/100 dilution + HepG2 cell lysate at 35 µg

Anti-Radical Fringe antibody - ab57919 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-RFNG Antibody (aa222-330) - LS-C126236 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-RFNG Antibody (aa282-310, AP) - LS-C268099 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa282-310, APC) - LS-C268100 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa282-310, Biotin) - LS-C268101 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa282-310, FITC) - LS-C268102 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa282-310, Azide-free, HRP) - LS-C268103 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa282-310, PE) - LS-C268104 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa282-310) - LS-C162553 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

RFNG Antibody western blot of HepG2 cell line lysates (35 ug/lane). The RFNG antibody detected the RFNG protein (arrow).

Anti-RFNG Antibody (aa68-312, Biotin) - LS-C297725 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa281-310) - LS-C324627 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa68-312, FITC) - LS-C304882 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-RFNG Antibody (aa82-191, Azide-free) - LS-C328579 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal

Anti-RFNG Antibody (clone 6C7) - LS-C198082 from LifeSpan Bioscience

Prices	$355.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	6C7

Immunofluorescence of monoclonal antibody to RFNG on HepG2 cell. [antibody concentration 10 ug/ml]

Anti-RFNG Antibody (aa68-312) - LS-C295709 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

RFNG Antibody - AF7198 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows recombinant human RFNG (10 ng/lane). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RFNG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7198) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RFNG at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

RFNG Antibody - NBP1-90946 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: RFNG Antibody [NBP1-90946] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

RFNG Antibody (6C7) - H00005986-M08 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	6C7

Western Blot: RFNG Antibody (6C7) [H00005986-M08] - Analysis of RFNG expression in HepG2 (Cat # L019V1).

RFNG Antibody - H00005986-A01 from Novus Biologicals

Prices	$269.00
Sizes	50 µl
Host	Mouse
Clonality	Polyclonal

Anti-RFNG antibody produced in rabbit - HPA011564 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-RFNG: <B>Cat. No. HPA011564</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human stomach tissue showing strong cytoplasmic positivity in glandular cells.

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