| Gene Symbol | GNAT3 |
|---|---|
| Entrez Gene | 346562 |
| Alt Symbol | GDCA |
| Species | Human |
| Gene Type | protein-coding |
| Description | guanine nucleotide binding protein, alpha transducing 3 |
| Other Description | guanine nucleotide-binding protein G(t) subunit alpha-3|gustducin alpha-3 chain |
| Swissprots | B9EJG5 A4D1B3 A4D1B2 A8MTJ3 |
| Accessions | A8MTJ3 EAL24192 EAL24193 EAW77007 BC147016 AAI47017 BC147017 AAI47018 XM_011516123 XP_011514425 XM_011516124 XP_011514426 XM_011516125 XP_011514427 NM_001102386 NP_001095856 |
| Function | Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intrace |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:8015379}. Note=Dual ditribution pattern; plasmalemmal pattern with apical region localization and cytosolic pattern with localization throughout the cytoplasm. |
| Tissue Specificity | Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and foliate papillae of the tongue at protein level. Expressed in enteroendocrine L cells of the gut. Detected also in spermatozoa. {ECO:0000269|PubMed:16728727, ECO:0000269|PubMed:17021831, ECO:0000269|PubMed:17724332, ECO:0000269|PubMed:8015379}. |
| Top Pathways | Taste transduction, Phototransduction, Carbohydrate digestion and absorption |
G α gust CRISPR/Cas9 KO Plasmid (h) - sc-415616 from Santa Cruz Biotechnology
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G α gust HDR Plasmid (h) - sc-415616-HDR from Santa Cruz Biotechnology
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G α gust Double Nickase Plasmid (h) - sc-415616-NIC from Santa Cruz Biotechnology
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G α gust Double Nickase Plasmid (h2) - sc-415616-NIC-2 from Santa Cruz Biotechnology
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G α gust CRISPR Activation Plasmid (h) - sc-415616-ACT from Santa Cruz Biotechnology
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G α gust CRISPR Activation Plasmid (h2) - sc-415616-ACT-2 from Santa Cruz Biotechnology
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G α gust Lentiviral Activation Particles (h) - sc-415616-LAC from Santa Cruz Biotechnology
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G α gust Lentiviral Activation Particles (h2) - sc-415616-LAC-2 from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU224361 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN9414 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND9414 from Sigma-Aldrich
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GNAT3 siRNA (Human) - CRJ7485 from Cohesion Biosciences
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GNAT3 - MBS8222806 from MyBioSource
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shRNA set against Human GNAT3 (NM_001102386.1) - SHH304722 from Creative biogene
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Human GNAT3 siRNA - orb277986 from Biorbyt
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GNAT3 shRNA Plasmid (Human) - CHJ7485 from Cohesion Biosciences
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