MSH6

Gene Symbol MSH6
Entrez Gene 2956
Alt Symbol GTBP, GTMBP, HNPCC5, HSAP, p160
Species Human
Gene Type protein-coding
Description mutS homolog 6
Other Description DNA mismatch repair protein Msh6|G/T mismatch-binding protein|mutS-alpha 160 kDa subunit|sperm-associated protein
Swissprots Q8TCX4 O43706 P52701 B4E3I4 F5H2F9 O43917 B4DF41 Q9BTB5
Accessions AAB47425 AAK21215 AAL87401 BAA23673 BAA23674 EAX00208 EAX00209 P52701 AK130683 AK293921 BAG57302 AK304735 BAG65496 AK308392 BC004246 AAH04246 BC071594 BC104665 AAI04666 D89646 BAA23675 DA857066 DQ892898 ABM83824 U28946 AAC50461 U54777 AAB39212 Y16676 CAC79990 XM_005264271 XP_005264328 XM_011532798 XP_011531100 XM_011532799 XP_011531101 XM_011532800 XP_011531102 NM_000179 NP_000170 NM_001281492 NP_001268421 NM_001281493 NP_001268422 NM_001281494 NP_001268423
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch re
Subcellular Location Nucleus {ECO:0000269|PubMed:23622243}. Chromosome {ECO:0000269|PubMed:23622243}. Note=Associates with H3K36me3 via its PWWP domain.
Top Pathways Mismatch repair, Colorectal cancer

MSH6 (D60G2) XP ® Rabbit mAb - 5424 from Cell Signaling Technology

Prices $277.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D60G2
Western blot analysis of extracts from various cell types using MSH6 (D60G2) XP ® Rabbit mAb.

MSH6 (P150) Antibody - 3995 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using MSH6 (P150) Antibody.

MSH6 (L990) Antibody - 3996 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using MSH6 (L990) Antibody.

MSH6 (3E1) Mouse mAb - 12988 from Cell Signaling Technology

Prices $250.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 3E1
Western blot analysis of extracts from various cell lines using MSH6 (3E1) Mouse mAb.

GTBP (F-1) - sc-271979 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GTBP (F-1): sc-271979. Western blot analysis of GTBP expression in HeLa nuclear extract.

GTBP (E-8) - sc-137015 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GTBP (E-8): sc-137015. Western blot analysis of GTBP expression in 293T whole cell lysate.

GTBP (H-140) - sc-25835 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GTBP (H-140): sc-25835. Immunofluorescence staining of normal mouse lymph node frozen section showing cytoplasmic staining.

GTBP (F-16) - sc-26894 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GTBP (F-16): sc-26894. Western blot analysis of GTBP expression in HeLa nuclear extract.

GTBP (N-20) - sc-1243 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GTBP (N-20): sc-1243. Western blot analysis of GTBP expression in HeLa (A) and A-431 (B) nuclear extracts.

GTBP (H-141) - sc-10798 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
GTBP (H-141): sc-10798. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining.

GTBP (Q-20) - sc-1242 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GTBP (Q-20): sc-1242. Western blot analysis of GTBP expression in HeLa (A) and A-431 (B) nuclear extracts.

Anti-MSH6 antibody - ab137457 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MSH6 antibody (ab137457) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

Anti-MSH6 antibody - ab175284 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling MSH6 with ab175284 at 1/100. Magnification: 200x.

Anti-MSH6 antibody - ab180832 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab180832. Blue DAPI for nuclear staining.

Anti-MSH6 antibody - ab10340 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node tissue labelling MSH6 with ab10340 at 1/500 (2µg/ml). Detection: DAB.

Anti-MSH6 antibody - ab69620 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MSH6 antibody (ab69620) at 1/500 dilutionLane 1 : Extracts from HUVEC cellsLane 2 : Extracts from HUVEC cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-MSH6 antibody - ab84426 from Abcam

Prices $376.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
ab84426, at 1/250 dilution, staining MSH6 in formalin-fixed, paraffin-embedded human seminoma by Immunohistochemistry using DAB detection and a Donkey anti-Goat IgG HRP conjugated secondary antibody.

Anti-MSH6 antibody [3A10H7] - ab66335 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3A10H7
Anti-MSH6 antibody [3A10H7] (ab66335) at 1/500 dilution + Immunogen (recombinant fragment)

Anti-MSH6 antibody [3E1] - ab124485 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3E1
Anti-MSH6 antibody [3E1] (ab124485) at 1/500 dilution + Human MSH6 recombinant protein

Anti-MSH6 antibody [44] - ab14204 from Abcam

Prices $387.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone 44
ab14204 staining human colon carcinoma by IHC-P.

Anti-MSH6 antibody [44], prediluted - ab59755 from Abcam

Prices $370.00
Sizes 6 ml
Host Mouse
Clonality Monoclonal
Clone 44

Anti-MSH6 antibody [EPR3945] - ab92471 from Abcam

Prices $401.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3945
Immunohistochemical staining of paraffin embedded rat liver with purified ab92471 at a dilution of 1/500. A pre-diluted HRP polymer for rabbit/mouse IgG was used as the secondary antibody and the sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-MSH6 antibody [EPR3945] (Alexa Fluor® 647) - ab198334 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3945
ab198334 staining MSH6 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198334 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

Anti-MSH6 antibody [SP93] - ab99889 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone SP93
Staining of MSH6 in a formalin fixed, paraffin embedded Human rectal carcinoma tissue using ab99889 at a dilution of 1/100.

Anti-MSH6 antibody [SP93], prediluted - ab99890 from Abcam

Prices $376.00
Sizes 7 ml
Host Rabbit
Clonality Monoclonal
Clone SP93
Staining of Human MSH6 in a Formalin/PFA-fixed paraffin-embedded section of Human Rectal Carcinoma using prediluted ab99890.
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