NOTCH4

Gene Symbol NOTCH4
Entrez Gene 4855
Alt Symbol INT3
Species Human
Gene Type protein-coding
Description notch 4
Other Description Notch homolog 4|neurogenic locus notch homolog protein 4
Swissprots Q9H3S8 B0V183 Q99458 Q5SSY7 B0V1X5 Q99466 Q9UIJ0 O00306 Q9UII9 Q99940
Accessions AAC63097 BAA13116 BAA88951 BAA88952 BAB20317 Q99466 AK131314 AK290785 BAF83474 AK293692 BAG57129 AK294161 BAG57484 AL705928 BC033869 BC063815 AAH63815 BC140782 AAI40783 BC144540 BC144541 BQ023880 D63395 BAA09708 U95299 AAC32288 NM_004557 NP_004548
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system (By similarity). {ECO:0000250}.
Subcellular Location Cell membrane; Single-pass type I membrane protein.
Tissue Specificity Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes.
Top Pathways Notch signaling pathway, MicroRNAs in cancer, Dorso-ventral axis formation

Notch4 (L5C5) Mouse mAb - 2423 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L5C5
Western blot analysis of the recombinant human ankyrin repeat domains of Notch1, 2, 3 and 4 (HANK1-4, lanes 1-4, respectively), using 5 ng of protein per lane and Notch4 (L5C5) Mouse mAb.

Notch 4 (A-12) - sc-393893 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Notch 4 (A-12): sc-393893. Western blot analysis of Notch 4 expression in JAR (A) and Jurkat (B) whole cell lysates.

Notch 4 (C-19) - sc-8644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 4 (C-19): sc-8644. Western blot analysis of human recombinant Notch 4 fusion protein.

Notch 4 (E-12) - sc-32613 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 4 (E-12): sc-32613. Western blot analysis of Notch 4 expression in 293T whole cell lysate.

Notch 4 (F-17) - sc-32612 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 4 (F-17): sc-32612. Western blot analysis of Notch 4 expression in Jurkat whole cell lysate.

Notch 4 (H-225) - sc-5594 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Notch 4 (H-225): sc-5594. Western blot analysis of human recombinant Notch 4 fusion protein.

Notch 4 (N-17) - sc-8643 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Notch 4 (N-17): sc-8643. Immunofluorescence staining of methanol-fixed JAR cells showing membrane localization.

Anti-NOTCH4 antibody - ab134831 from Abcam

Prices $379.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Overlay histogram showing A549 cells stained with ab134831 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134831, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-NOTCH4 antibody - ab121004 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal

Anti-NOTCH4 antibody - ab33163 from Abcam

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NOTCH4 antibody (ab33163) at 1 µg/ml + Kidney (Human) Tissue Lysate - adult normal tissue (ab30203) at 20 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NOTCH4 antibody - ab139316 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NOTCH4 antibody (ab139316) at 1/500 dilution + Jurkat total cell extract

Anti-NOTCH4 antibody - ab91621 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NOTCH4 antibody (ab91621) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)Lysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-NOTCH4 antibody [EPNCIR101B] - ab166605 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR101B
Anti-NOTCH4 antibody [EPNCIR101B] (ab166605) at 1/100 dilution + Mouse lung lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-NOTCH4 antibody [EPR1246(2)] - ab170951 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1246(2)
All lanes : Anti-NOTCH4 antibody [EPR1246(2)] (ab170951) at 1/10000 dilutionLane 1 : HACAT cell lysateLane 2 : HUVEC cell lysateLane 3 : Human fetal heart lysateLane 4 : Saos-2 cell lysate

Anti-NOTCH4 antibody - BSA and Azide free - ab170989 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-NOTCH4 antibody - BSA and Azide free - ab170992 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-NOTCH4 antibody - C-terminal - ab199295 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NOTCH4 antibody - C-terminal (ab199295) at 1/500 dilutionLane 1 : Human liver cancer lysateLane 2 : Human kidney lysateLysates/proteins at 40 µg per lane.

Anti-NOTCH4 Antibody (aa371-420) IHC-plus™ - LS-B3498 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NOTCH4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3498 concentration 5 ug/ml.

Anti-NOTCH4 Antibody (aa1778-2003) - LS-C37776 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-NOTCH4 Antibody (aa1778-2003) - LS-C37775 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Rat
Clonality Monoclonal

Anti-NOTCH4 Antibody (aa1984-2002) - LS-C83740 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NOTCH4 Antibody (aa5708-6217) - LS-C91117 from LifeSpan Bioscience

Prices $455.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-NOTCH4 Antibody (clone HMN4-14, PE) - LS-C106197 from LifeSpan Bioscience

Prices $245.00, $355.00
Sizes 25 µg, 100 µg
Host Hamster
Clonality Monoclonal
Clone HMN4-14
Staining of CD8+CD11c+-gated C57Bl/6 splenocytes with 0.25 ug of PE Armenian Hamster IgG isotype control (blue histogram) or 0.5 ug of PE anti-mouse Notch4 (HMN4-14) (purple histogram). Total viable cells were used for analysis.

Anti-NOTCH4 Antibody (aa1261-1273) - LS-C154837 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-NOTCH4 Antibody (clone 12G39) - LS-C182625 from LifeSpan Bioscience

Prices $445.00
Sizes 250 µg
Host Hamster
Clonality Monoclonal
Clone 12G39
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