Gene Symbol | UHRF1 |
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Entrez Gene | 29128 |
Alt Symbol | ICBP90, Np95, RNF106, hNP95, hUHRF1, huNp95 |
Species | Human |
Gene Type | protein-coding |
Description | ubiquitin-like with PHD and ring finger domains 1 |
Other Description | E3 ubiquitin-protein ligase UHRF1|RING finger protein 106|inverted CCAAT box-binding protein of 90 kDa|nuclear phosphoprotein 95|nuclear protein 95|nuclear zinc finger protein Np95|transcription factor ICBP90|ubiquitin-like PHD and RING finger domain-containing protein 1 |
Swissprots | Q9P1U7 Q2HIX7 Q9P115 Q8J022 B2RBA9 A0JBR2 Q9H6S6 Q96T88 A8K024 |
Accessions | AAF64067 AAV40831 BAC20576 CCQ43417 EAW69187 EAW69188 EAW69189 Q96T88 AA026356 AB177623 BAF36719 AB177624 BAF36720 AF129507 AAF28469 AF274048 AAK55744 AK025578 BAB15177 AK074377 AK289389 BAF82078 AK303361 BAG64421 AK307239 AK314579 BAG37156 BC039136 BC046179 BC113875 AAI13876 CN335888 DA451120 EF560733 ABQ59043 EU446794 ABZ92323 HY074450 XM_011527942 XP_011526244 NM_001048201 NP_001041666 NM_001290050 NP_001276979 NM_001290051 NP_001276980 NM_001290052 NP_001276981 NM_013282 NP_037414 |
Function | Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the |
Subcellular Location | Nucleus {ECO:0000255|PROSITE- ProRule:PRU00358, ECO:0000269|PubMed:10646863, ECO:0000269|PubMed:17673620, ECO:0000269|PubMed:17967883, ECO:0000269|PubMed:19056828, ECO:0000269|PubMed:21777816}. Note=Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions. |
Tissue Specificity | Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer. {ECO:0000269|PubMed:10646863, ECO:0000269|PubMed:12838312, ECO:0000269|PubMed:15361834}. |
UHRF1 CRISPR/Cas9 KO Plasmid (h) - sc-403851 from Santa Cruz Biotechnology
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UHRF1 HDR Plasmid (h) - sc-403851-HDR from Santa Cruz Biotechnology
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UHRF1 Double Nickase Plasmid (h) - sc-403851-NIC from Santa Cruz Biotechnology
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UHRF1 Double Nickase Plasmid (h2) - sc-403851-NIC-2 from Santa Cruz Biotechnology
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UHRF1 CRISPR Activation Plasmid (h) - sc-403851-ACT from Santa Cruz Biotechnology
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UHRF1 CRISPR Activation Plasmid (h2) - sc-403851-ACT-2 from Santa Cruz Biotechnology
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UHRF1 Lentiviral Activation Particles (h) - sc-403851-LAC from Santa Cruz Biotechnology
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UHRF1 Lentiviral Activation Particles (h2) - sc-403851-LAC-2 from Santa Cruz Biotechnology
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UHRF1 siRNA (h) - sc-76805 from Santa Cruz Biotechnology
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UHRF1 shRNA Plasmid (h) - sc-76805-SH from Santa Cruz Biotechnology
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UHRF1 shRNA (h) Lentiviral Particles - sc-76805-V from Santa Cruz Biotechnology
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UHRF1 RNAi - H00029128-R01 from Novus Biologicals
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MISSION® esiRNA - EHU040501 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22478 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22478 from Sigma-Aldrich
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UHRF1 siRNA (Human) - CRH9216 from Cohesion Biosciences
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UHRF1 - MBS8223752 from MyBioSource
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shRNA set against Human UHRF1(NM_013282.3) - SHL113984 from Creative biogene
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shRNA set against Human UHRF1(NM_001048201.1) - SHL114020 from Creative biogene
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Human UHRF1 siRNA - orb269718 from Biorbyt
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UHRF1 shRNA Plasmid (Human) - CHH9216 from Cohesion Biosciences
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UHRF1 miRNA 3'UTR clone - MiUTR1H-10999 from Creative biogene
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UHRF1 miRNA 3'UTR clone - MiUTR1H-11000 from Creative biogene
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