OGT

Gene Symbol OGT
Entrez Gene 8473
Alt Symbol HRNT1, O-GLCNAC
Species Human
Gene Type protein-coding
Description O-linked N-acetylglucosamine (GlcNAc) transferase
Other Description O-GlcNAc transferase p110 subunit|O-GlcNAc transferase subunit p110|O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)|O-linked N-acetylglucosamine transferase 110 kDa subunit|UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit|UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase|uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
Swissprots Q8WWM8 O15294 Q96CC1 Q7Z3K0 Q9UG57
Accessions CAC86127 CAC86128 CAC86129 EAX05285 EAX05286 EAX05287 O15294 AF070560 AF147394 AF223393 AAF31458 AK300267 BAG62028 AK313530 AL049950 AL050366 CAB62528 AL833085 CAD89970 BC014434 AAH14434 BC015144 BC038180 AAH38180 BX537844 CAD97853 CB857907 DA460761 DQ893623 ABM84549 DQ896848 ABM87847 U77413 AAB63466 XM_005262308 XP_005262365 NM_181672 NP_858058 NM_181673 NP_858059 NM_003605
Function Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta- linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin s
Subcellular Location Isoform 4: Cytoplasm. Nucleus.
Tissue Specificity Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. {ECO:0000269|PubMed:9083068}.
Top Pathways Other types of O-glycan biosynthesis

O-GlcNAc (CTD110.6) Mouse mAb (HRP Conjugate) - 12938 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone CTD110.6
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with PUGNAc (+), an inhibitor of N-acetyl-β-D-glucosaminidase, using O-GlcNAc (CTD110.6) Mouse mAb (HRP Conjugate).

O-GlcNAc (CTD110.6) Mouse mAb - 9875 from Cell Signaling Technology

Prices $261.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone CTD110.6
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with PUGNAc (+), an inhibitor of N-acetyl-β-D-glucosaminidase, using O-GlcNAc (CTD110.6) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

OGT Antibody - 5368 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using OGT Antibody.

O-GlcNAc (CTD110.6) - sc-59623 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
O-GlcNAc (CTD110.6): sc-59623. Western blot analysis of O-GlcNAc expression in HeLa nuclear extract (A) and A549 whole cell lysate (B) and mouse brain tissue extract (C).

O-GlcNAc (10D8 ) - sc-81483 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal

O-GlcNAc transferase (C-10) - sc-376253 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
O-GlcNAc transferase (C-10): sc-376253. Western blot analysis of O-GlcNAc transferase expression in SK-N-MC nuclear extract.

O-GlcNAc transferase (A-6) - sc-74547 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
O-GlcNAc transferase (A-6): sc-74547. Western blot analysis of O-GlcNAc transferase expression in SK-N-MC nuclear extract (A) and SK-N-MC (B), A549 (C), c4 (D) and rat PBL (E) whole cell lysates.

O-GlcNAc transferase (F-12) - sc-74546 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
O-GlcNAc transferase (F-12): sc-74546. Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. Kindly provided by Yang Xiang, Ph.D., Division of Newborn Medicine, Boston Childrens Hospital, Cell Biology Department, Harvard Medical School.

O-GlcNAc transferase (D-20) - sc-22625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

O-GlcNAc transferase (H-300) - sc-32921 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
O-GlcNAc transferase (H-300): sc-32921. Western blot analysis of O-GlcNAc transferase expression in rat PBL (A), A549 (B) and HeLa (C) whole cell lysates and HeLa nuclear extract (D) and mouse brain tissue extract (E).

O-GlcNAc transferase (V-18) - sc-22628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
O-GlcNAc transferase (V-18): sc-22628. Western blot analysis of O-GlcNAc transferase expression in SK-N-SH whole cell lysate.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody - ab185813 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling OGT / O-Linked N-Acetyl glucosamine Transferase with ab185813 at 1/100. Magnification: 400x.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody - ab50271 from Abcam

Prices $372.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody - ab50270 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Cytoplasmic, and nuclear localization of O-Linked N-Acetylglucosamine in human lung carcinoma (A549) cells stained by ab50270. A. Working dilution of 1:100 B. Working dilution of 1:50

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody - ab50273 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12712] - ab181361 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12712
All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12712] (ab181361) at 1/1000 dilutionLane 1 : A549 cell lysatesLane 2 : U87-MG cell lysatesLane 3 : HeLa cell lysatesLane 4 : 293T cell lysatesLysates/proteins at 10 µg per lane.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] - ab177941 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12713
All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/1000 dilutionLane 1 : A549 cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLane 4 : U87-MG cell lysateLysates/proteins at 10 µg per lane.

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (Alexa Fluor® 488) - ab198530 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12713
ab198530 staining OGT/O-linked N-Acetylglucosamine Transferase in A549 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198530 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (Alexa Fluor® 647) - ab198532 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12713
ab198532 staining OGT/O-Linked N-Acetylglucosamine Transferase in A549 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198532 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody - N-terminal (ab200124) at 1/1000 dilutionLane 1 : Total cell extracts from mouse brainLane 2 : Total cell extracts from mouse thymus

Anti-OGT / O-GLCNAC Antibody (aa223-502) - LS-C186114 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded Cal27 Xenograft using O-GlcNAc transferase antibody at 1:100 dilution.

Anti-OGT / O-GLCNAC Antibody - LS-C341397 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-OGT / O-GLCNAC Antibody (N-Terminus) - LS-C81349 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal heart lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.

Anti-OGT / O-GLCNAC Antibody (N-Terminus) - LS-C345233 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of OGT Antibody with human MDA-MB-435s Whole Cell lysate.

Anti-OGT / O-GLCNAC Antibody (aa1017-1046) - LS-C163114 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human brain tissue reacted with OGT Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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