ARHGEF12

Gene Symbol ARHGEF12
Entrez Gene 23365
Alt Symbol LARG, PRO2792
Species Human
Gene Type protein-coding
Description Rho guanine nucleotide exchange factor (GEF) 12
Other Description leukemia-associated RhoGEF|leukemia-associated rho guanine nucleotide exchange factor|rho guanine nucleotide exchange factor 12
Swissprots Q9NZN5 O15086 Q6P526
Accessions EAW67508 EAW67509 EAW67510 Q9NZN5 AB002380 BAA20836 AF119898 AAF69652 AF180681 AAF36817 AK294803 BAG57923 AK304316 BAG65168 AL137456 BC031784 BC063117 AAH63117 BC152900 AAI52901 BX647785 BX648515 DC350360 XM_005271478 XP_005271535 XM_006718805 XP_006718868 XM_011542720 XP_011541022 NM_001198665 NP_001185594 NM_001301084 NP_001288013 NM_015313 NP_056128
Function May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13. {ECO:0000269|PubMed:11094164}.
Subcellular Location Cytoplasm {ECO:0000305}. Membrane {ECO:0000305}. Note=Translocated to the membrane upon stimulation. {ECO:0000305}.
Tissue Specificity Ubiquitously expressed. Isoform 2 is found in jejunum and testis.
Top Pathways Axon guidance, Vascular smooth muscle contraction, Tuberculosis, Proteoglycans in cancer, Regulation of actin cytoskeleton

LARG (H-3) - sc-166318 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
LARG (H-3): sc-166318. Western blot analysis of LARG expression in HL-60 whole cell lysate.

LARG (P-16) - sc-15440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

LARG (H-70) - sc-25638 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
LARG (H-70): sc-25638. Western blot analysis of LARG expression in HeLa whole cell lysate.

LARG (N-14) - sc-15439 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
LARG Antibody (N-14): sc-15439. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.

Anti-LARG antibody - ab198915 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling LARG with ab198915 at a 1/20 dilution.

Anti-LARG antibody - ab86095 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LARG antibody (ab86095) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Anti-LARG antibody - ab136072 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-LARG antibody (ab136072) at 1/500 dilution + HUVEC cell lysate at 30 µg

Anti-ARHGEF12 Antibody (aa1494-1544) - LS-C288719 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF12 Antibody (aa1350-1400) - LS-C288718 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human LARG by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-LARG antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-ARHGEF12 Antibody (aa435-484) IHC-plus™ - LS-B11451 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ARHGEF12 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11451 concentration 20 ug/ml.

Anti-ARHGEF12 Antibody (aa449-498) - LS-C120330 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from RAW264.7 cells, using ARHGEF12 Antibody. The lane on the right is treated with the synthesized peptide.

LARG Antibody - AF4737 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human LARG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LARG at approximately 200 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

LARG Antibody - NBP1-89619 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: LARG Antibody [NBP1-89619] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.

LARG Polyclonal Antibody - bs-4213R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ARHGEF12 antibody produced in rabbit - SAB4500976 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ARHGEF12: <B>Cat. No. SAB4500976</B>: Immunoblotting analysis of extracts from RAW264.7 cells, using Anti-ARHGEF12 antibody. The lane on the left was treated with the Anti-ARHGEF12 antibody. The lane on the right (negative control) was treated with both Anti-ARHGEF12 antibody and the synthesized immunogen peptide.

ARHGEF12 antibody - GTX87286 from GeneTex

Prices $319.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Anti-ARHGEF12 antibody used in Western blot. GTX87286

Human LARG Antibody - AF4737 from R&D Systems

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Detection of Human LARG by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human LARG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017 ). A specific band was detected for LARG at approximately 200 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2 .

Human LARG Affinity Purified Polyclonal Ab, Goat IgG - AF4737-SP from R&D Systems

Host Goat
Clonality Polyclonal
Detection of Human LARG by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human LARG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for LARG at approximately 200 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

ARHGEF12 Antibody (OAAF04189) - OAAF04189 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

ARHGEF12 Antibody (OAAF06112) - OAAF06112 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

LARG Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-4213R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LARG Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-4213R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LARG Polyclonal Antibody, ALEXA FLUOR® 555 Conjugated - bs-4213R-A555 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LARG Polyclonal Antibody, ALEXA FLUOR® 647 Conjugated - bs-4213R-A647 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

LARG Polyclonal Antibody, Cy3 Conjugated - bs-4213R-Cy3 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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