| Gene Symbol |
TOP1
|
| Entrez Gene |
7150
|
| Alt Symbol |
TOPI
|
| Species |
Human
|
| Gene Type |
protein-coding
|
| Description |
topoisomerase (DNA) I
|
| Other Description |
DNA topoisomerase 1|type I DNA topoisomerase
|
| Swissprots |
E1P5W3 O43256 A8KA78 Q9UJN0 Q12856 Q12855 Q15610 P11387 Q5TFY3
|
| Accessions |
AAA61206 CAA36834 EAW75994 EAW75995 EAW75996 EAW75997 P11387 AK225095 AK292943 BAF85632 AK310508 AK310516 BC000943 AAH00943 BC004475 AAH04475 BC039009 BC056681 BC067349 AAH67349 BC136297 AAI36298 J03250 AAA61207 M27913 AAA61208 U07804 AAB60379 U07806 AAB60380 X16479 CAA34500 XM_011529032 XP_011527334 XM_011529033 XP_011527335 NM_003286 NP_003277
|
| Function |
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on
|
| Subcellular Location |
Nucleus, nucleolus {ECO:0000269|PubMed:12149243}. Nucleus, nucleoplasm {ECO:0000269|PubMed:12149243}. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
|
| Tissue Specificity |
Endothelial cells. {ECO:0000269|PubMed:19168442}.
|
![Western Blot: Topoisomerase I Overexpression Lysate (Adult Normal) [NBL1-17194] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Topoisomerase I.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/7419_Topoisomerase%2520I%2520Overexpression%2520Lysate%2520(Adult%2520Normal)-Western%2520Blot-NBL1-17194-img0002.jpg)