AKT2

Gene Symbol AKT2
Entrez Gene 208
Alt Symbol HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
Species Human
Gene Type protein-coding
Description v-akt murine thymoma viral oncogene homolog 2
Other Description PKB beta|RAC-PK-beta|RAC-beta serine/threonine-protein kinase|murine thymoma viral (v-akt) homolog-2|protein kinase Akt-2|protein kinase B beta|rac protein kinase beta
Swissprots P31751 Q68GC0 Q0VAN1 Q0VAN0 Q05BV0 B2RBD8
Accessions AAT97984 EAW56941 EAW56942 EAW56943 P31751 AK054771 AK055779 BAG51573 AK122839 AK294453 BAG57690 AK304209 BAH14135 AK308006 AK308906 AK314619 BAG37185 BC022779 AAH22779 BC032709 AAH32709 BC040028 BC063421 AAH63421 BC120994 AAI20995 BC120995 AAI20996 BM675176 BQ219902 CN351354 M77198 AAA36585 M95936 AAA58364 XM_011526614 XP_011524916 XM_011526615 XP_011524917 XM_011526616 XP_011524918 XM_011526617 XP_011524919 XM_011526618 XP_011524920 XM_011526619 XP_011524921 XM_011526620 XP_011524922 XM_011526621 XP_011524923 XM_011526622 XP_011524924 NM_001243027 NP_001229956 NM_001243028 NP_001229957 NM_001626 NP_001617
Function AKT2 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glyco
Subcellular Location Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Note=Localizes within both nucleus and cytoplasm of proliferative primary myoblasts and mostly within the nucleus of differentiated primary myoblasts. By virtue of the N-terminal PH domain, is recruited to sites of the plasma membrane containing increased PI(3,4,5)P3 or PI(3,4)P2, cell membrane targeting is also facilitared by interaction with CLIP3.
Tissue Specificity Expressed in all cell types so far analyzed.
Top Pathways Apoptosis, Tight junction, VEGF signaling pathway, Focal adhesion, Toll-like receptor signaling pathway

PathScan ® Phospho-Akt2 (Ser474) Sandwich ELISA Kit - 7048 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of A-673 cells with insulin stimulates phosphorylation of Akt2 at Ser474, detected by PathScan ® Phospho-Akt2 (Ser474) Sandwich ELISA Kit #7048, but does not affect levels of total Akt2 protein detected by PathScan ® Total Akt2 Sandwich ELISA Kit #7046. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Akt2 (5B5) Rabbit mAb #2964 (left panel) and Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (right panel) are shown in the bottom figure.

PathScan ® Phospho-Akt2 (Ser474) Sandwich ELISA Kit (Mouse Preferred) - 7932 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of NIH/3T3 cells with PDGF #9909 stimulates phosphorylation of Akt2 at Ser474, detected by PathScan ® Phospho-Akt2 (Ser474) Sandwich ELISA Kit (Mouse Preferred) #7932, but does not affect levels of total Akt2 protein detected by PathScan ® Total Akt2 Sandwich ELISA kit (Mouse Preferred) #7930. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Akt2 (5B5) Rabbit mAb #2964 (left panel) and Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 (right panel) are shown in the bottom figure.

PathScan ® Total Akt2 Sandwich ELISA Kit (Mouse Preferred) - 7930 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of NIH/3T3 cells with PDGF #9909 stimulates phosphorylation of Akt2 at Ser474, detected by PathScan ® Phospho-Akt2 (Ser474) Sandwich ELISA Kit (Mouse Preferred) #7932, but does not affect levels of total Akt2 protein detected by PathScan ® Total Akt2 Sandwich ELISA kit (Mouse Preferred) #7930. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Akt2 (5B5) Rabbit mAb #2964 (left panel) and Phospho-Akt (Ser473) (193H12) Rabbit mAb #4058 (right panel) are shown in the bottom figure.

PathScan ® Total Akt2 Sandwich ELISA Kit - 7046 from Cell Signaling Technology

Category ELISA Kit
Prices $489.00
Sizes 1 kit (96 assays)
Figure 1: Treatment of A-673 cells with insulin stimulates phosphorylation of Akt2 at Ser474, detected by PathScan ® Phospho-Akt2 (Ser474) Sandwich ELISA Kit #7048, but does not affect levels of total Akt2 protein detected by PathScan ® Total Akt2 Sandwich ELISA Kit #7046. The absorbance readings at 450 nm are shown in the top figure, while the corresponding western blots using Akt2 (5B5) Rabbit mAb #2964 (left panel) and Phospho-Akt (Ser473) (D9E) XP ® Rabbit mAb #4060 (right panel) are shown in the bottom figure.

HTScan® Akt2 Kinase Assay Kit - 7504 from Cell Signaling Technology

Category Assay Kit
Figure 1. Akt2 kinase activity was measured in a radiometric assay using the following reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: R11-GSK3 (14-27), 5 µg/50 µl, recombinant Akt2: 100 ng/50 µl.

Akt (pS473) + total Akt ELISA Kit - ab126433 from Abcam

Category ELISA Kit
Prices $499.00
Sizes 1 x 96 tests
MCF7 cells were stimulated for five minutes with 1 ug x mL-1 of insulin (ab123768). Cell lysates were assessed for total Akt and Akt(pS473), shown as OD (450 nm) after background signal was subtracted (duplicates +/- SD).
Jurkat cells were harvested by centrifugation, resuspended in HBSS, and seeded at 10K/well (20 µL/well) in a rinsed PhosphoTracer assay plate. The cells were treated with various concentrations of wortmannin (10 µL/well) for 1 h at 37°C, then treated with 5% FCS (10 µL/well) for 15 min. The cells were lysed with 10 µL/well Lysis Mix Concentrate, with shaking for 10 min. Antibody Mix specific for phospho AKT (50 µL/well) was added to the lysate, and the plates were incubated for 1 hr at room temp, with shaking. Plates were washed and Substrate Mix was added. The plates were covered in foil and incubated for 10 min with shaking. Signal in the wells was determined using a plate reader.

AKT 1/2/3 (pS473) + AKT1 (Total) SimpleStep ELISA™ Kit - ab176657 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml

AKT 1/2/3 (pT308) + AKT1 (Total) SimpleStep ELISA™ Kit - ab176658 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml

AKT 1/2/3 (pT308) PhosphoTracer ELISA Kit - ab119615 from Abcam

Category ELISA Kit
Sensitivity 50 pg/ml
Range 100 pg/ml - 10000 pg/ml
Detection of AKT 1/2/3 (pT308) in MCF7 cells treated with various concentration of wortmannin, using ab119615.

AKT 1/2/3 (pT308) SimpleStep ELISA™ Kit - ab176636 from Abcam

Category ELISA Kit
Prices $484.00
Sizes 1 x 96 tests
Sensitivity 30 pg/ml
Range 1 ng/ml - 100 ng/ml

AKT total + Phospho S473 In-Cell ELISA Kit (IR) - ab126579 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Figure 1. Normalized IR signal in PDGF induced NIH3T3 cells. NIH3T3 cells were seeded at 40,000 cells per well and allowed to adhere for 2 hours prior to serum starvation and induction of phosphorylation with a dose-response of PDGF recombinant protein. At maximum doses, PDGF induced a 5 fold increase in the levels of Akt-1 phosphorylation.

AKT total + Phospho S473 In-Cell ELISA kit (Colorimetric) - ab126578 from Abcam

Category ELISA Kit
Prices $448.00
Sizes 1 x 96 tests
Figure 1. Normalized IR signal in PDGF induced NIH3T3 cells. NIH3T3 cells were seeded at 40,000 cells per well and allowed to adhere for 2 hours prior to serum starvation and induction of phosphorylation with a dose-response of PDGF recombinant protein. At maximum doses, PDGF induced a 5 fold increase in the levels of Akt-1 phosphorylation.

Human AKT2 ELISA Kit (Sandwich ELISA) - LS-F4927 from LifeSpan Bioscience

Category ELISA Kit
Prices $690.00
Sizes 1 plate
Sensitivity 0.313 ng/ml
Range 0.313-20 ng/ml

Mouse/Human/Rat AKT2 ELISA Kit (Cell-Based ELISA) - LS-F1789 from LifeSpan Bioscience

Category ELISA Kit
Prices $450.00
Sizes 1 plate
Range 0.313-20 ng/ml

Mouse/Human/Rat Phospho-AKT2 (Ser474) ELISA Kit (Cell-Based Phosphorylation ELISA) - LS-F1448 from LifeSpan Bioscience

Category ELISA Kit
Prices $550.00
Sizes 1 plate
Range 0.313-20 ng/ml

Rat AKT2 ELISA Kit (Sandwich ELISA) - LS-F8422 from LifeSpan Bioscience

Category ELISA Kit
Prices $740.00
Sizes 1 plate
Sensitivity 0.114 ng/ml
Range 0.313-20 ng/ml

Rabbit anti-AKT2 Antibody, Affinity Purified - PLA0245 from Sigma-Aldrich

Prices $323.50
Sizes 100 µl
<B>Immunofluorescence</B>: This antibody is qualified for the Proximity Ligation Assay (PLA). The above image shows representative results for PLA using three color fluorescence, including DAPI stained nuclei (blue), phalloidin stained cytoplasmic F-actin (green), and PLA positive signal (red).

Human Total Akt2 DuoSet IC ELISA, 2 Plate - DYC2315-2 from R&D Systems

Category ELISA Kit
Prices $329.00
Sizes 1 kit (for 2 Plates)
Range 469.00 - 30,000 pg/mL

Protein Kinase B Beta (PKBb), Human, ELISA Kit - SEB719Hu from ARP American Research Products

Category ELISA Kit
Prices $685.00
Sizes 96 t
Sensitivity 0.132ng/mL
Range 0.312-20ng/mL

v-akt murine thymoma viral oncogene homolog 2, ELISA Kit - MBS9334811 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Akt2 Colorimetric Cell-Based ELISA Kit - CB5031 from Assay Biotechnology

Category ELISA Kit
Prices $380.00, $760.00, $1,140.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

Akt2 (Phospho-Ser474) Colorimetric Cell-Based ELISA Kit - CBP1491 from Assay Biotechnology

Category ELISA Kit
Prices $480.00, $960.00, $1,440.00
Sizes 1 kit, 2 kits, 3 kits
Range > 5000 Cells

ELISA Kit for Protein Kinase B Beta (PKBb) - SEB719Hu from Uscn Life Science Inc.

Category ELISA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.132ng/mL.
Range 0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL

ELISA Kit for Protein Kinase B Beta (PKBb) - SEB719Ra from Uscn Life Science Inc.

Category ELISA Kit
Sensitivity The minimum detectable dose of this kit is typically less than 0.114ng/mL.
Range 0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL
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