Gene Symbol | MED15 |
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Entrez Gene | 51586 |
Alt Symbol | ARC105, CAG7A, CTG7A, PCQAP, TIG-1, TIG1, TNRC7 |
Species | Human |
Gene Type | protein-coding |
Description | mediator complex subunit 15 |
Other Description | CTG repeat protein 7a|PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein|PC2 glutamine/Q-rich-associated protein|PC2-glutamine-rich-associated protein|TPA inducible gene-1|TPA inducible protein|TPA-inducible gene 1 protein|activator-recruited cofactor 105 kDa component|activator-recruited cofactor, 105-kD|mediator of RNA polymerase II transcription subunit 15|positive cofactor 2 glutamine/Q-rich-associated protein|positive cofactor 2, glutamine/Q-rich-associated protein|trinucleotide repeat containing 7|trinucleotide repeat-containing gene 7 protein |
Swissprots | Q96IH7 O15413 Q96RN5 Q6IC31 D3DX31 Q9P1T3 D3DX32 Q8NF16 Q96CT0 |
Accessions | EAX02948 EAX02949 EAX02950 EAX02951 EAX02952 EAX02953 EAX02954 EAX02955 EAX02956 Q96RN5 AF056191 AAC12944 AF328769 AAK58423 AK000003 BAA92228 AK074230 AK074268 BAB85034 AK090465 BAC03446 AK124276 BAG54024 AK124925 BAG54113 AK126208 AK294540 BAG57747 AK294734 BAH11863 AK297284 BAG59755 AK297789 BAG60130 AK315540 AL832151 CAH10580 AL832923 CAH10623 AW245653 BC003078 AAH03078 BC005027 BC007529 AAH07529 BC013985 AAH13985 BC017110 BX478424 CB156495 CB988805 CR456537 CAG30423 CU013137 CAK54568 CU013425 CAK54867 DA182752 KF274544 KF274593 U80745 AAB91443 XM_006724263 XP_006724326 XM_006724264 XP_006724327 XM_011530214 XP_011528516 XM_011530215 XP_011528517 XM_011530216 XP_011528518 XM_011530217 XP_011528519 XM_011530218 XP_011528520 XM_011530219 XP_011528521 XM_011530220 XP_011528522 XM_011530221 XP_011528523 NM_001003891 NP_001003891 NM_001293234 NP_001280163 NM_001293235 NP_001280164 NM_001293236 NP_001280165 NM_001293237 NP_001280166 NM_015889 NP_056973 |
Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. {ECO:0000269|PubMed:12167862, ECO:0000269|PubMed:16630888, ECO:0000269|PubMed:16799563}. |
Subcellular Location | Cytoplasm. Nucleus. |
Tissue Specificity | Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney. {ECO:0000269|PubMed:11024300}. |
Med15 CRISPR/Cas9 KO Plasmid (h) - sc-405129 from Santa Cruz Biotechnology
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Med15 HDR Plasmid (h) - sc-405129-HDR from Santa Cruz Biotechnology
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Med15 Double Nickase Plasmid (h) - sc-405129-NIC from Santa Cruz Biotechnology
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Med15 Double Nickase Plasmid (h2) - sc-405129-NIC-2 from Santa Cruz Biotechnology
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Med15 CRISPR Activation Plasmid (h) - sc-405129-ACT from Santa Cruz Biotechnology
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Med15 CRISPR Activation Plasmid (h2) - sc-405129-ACT-2 from Santa Cruz Biotechnology
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Med15 Lentiviral Activation Particles (h) - sc-405129-LAC from Santa Cruz Biotechnology
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Med15 Lentiviral Activation Particles (h2) - sc-405129-LAC-2 from Santa Cruz Biotechnology
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Med15 siRNA (h) - sc-75767 from Santa Cruz Biotechnology
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Med15 shRNA Plasmid (h) - sc-75767-SH from Santa Cruz Biotechnology
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Med15 shRNA (h) Lentiviral Particles - sc-75767-V from Santa Cruz Biotechnology
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MED15 RNAi - H00051586-R02 from Novus Biologicals
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MED15 RNAi - H00051586-R01 from Novus Biologicals
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MISSION® esiRNA - EHU059031 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN13164 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND13164 from Sigma-Aldrich
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MED15 siRNA (Human) - CRH9845 from Cohesion Biosciences
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shRNA set against Human MED15 (NM_015889.3) - SHH339919 from Creative biogene
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shRNA set against Human PCQAP(NM_015889.3) - SHH218941 from Creative biogene
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shRNA set against Human PCQAP(NM_001003891.1) - SHH218959 from Creative biogene
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Human MED15 siRNA - orb270347 from Biorbyt
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MED15 shRNA Plasmid (Human) - CHH9845 from Cohesion Biosciences
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MED15 miRNA 3'UTR clone - MiUTR1H-07679 from Creative biogene
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MED15 miRNA 3'UTR clone - MiUTR1H-07680 from Creative biogene
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