Gene Symbol | ZRANB3 |
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Entrez Gene | 84083 |
Alt Symbol | 4933425L19Rik, AH2 |
Species | Human |
Gene Type | protein-coding |
Description | zinc finger, RAN-binding domain containing 3 |
Other Description | DNA annealing helicase and endonuclease ZRANB3|annealing helicase 2|zinc finger Ran-binding domain-containing protein 3 |
Swissprots | D3DP76 B5MDI3 Q53SM1 B3KYA1 Q9H0E8 B4E375 Q5FWF4 E9PBP0 Q8N1P4 Q6P2C4 |
Accessions | AAX93066 AAY14765 EAX11629 EAX11630 EAX11631 EAX11632 EAX11633 EAX11634 EAX11635 EAX11636 Q5FWF4 AI670931 AK095362 BAC04536 AK131303 BAG54763 AK304601 BAG65387 AL136824 CAB66758 AL833322 AM392694 CAL37572 AM392702 CAL37580 BC089429 AAH89429 BX647838 XM_005263809 XP_005263866 XM_005263811 XP_005263868 XM_005263812 XP_005263869 XM_006712788 XP_006712851 XM_011511958 XP_011510260 XM_011511959 XP_011510261 XM_011511960 XP_011510262 XM_011511961 XP_011510263 XM_011511962 XP_011510264 XM_011511963 XP_011510265 XM_011511964 XP_011510266 XM_011511965 XP_011510267 XM_011511966 XP_011510268 XM_011511967 XP_011510269 NM_001286568 NP_001273497 NM_001286569 NP_001273498 NM_032143 NP_115519 |
Function | DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events. Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase. In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks. {ECO:0000269|PubMed:21078962, ECO:0000269|PubMed:22704558, ECO:0000269|PubMed:22705370, ECO:0000269|PubMed:22759634}. |
Subcellular Location | Nucleus. Chromosome. Note=Following DNA damage, recruited to sites of DNA damage and stalled replication forks by polyubiquitinated PCNA. |
ZRANB3 CRISPR/Cas9 KO Plasmid (h) - sc-405985 from Santa Cruz Biotechnology
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ZRANB3 HDR Plasmid (h) - sc-405985-HDR from Santa Cruz Biotechnology
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ZRANB3 Double Nickase Plasmid (h) - sc-405985-NIC from Santa Cruz Biotechnology
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ZRANB3 Double Nickase Plasmid (h2) - sc-405985-NIC-2 from Santa Cruz Biotechnology
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ZRANB3 CRISPR Activation Plasmid (h) - sc-405985-ACT from Santa Cruz Biotechnology
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ZRANB3 CRISPR Activation Plasmid (h2) - sc-405985-ACT-2 from Santa Cruz Biotechnology
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ZRANB3 Lentiviral Activation Particles (h) - sc-405985-LAC from Santa Cruz Biotechnology
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ZRANB3 Lentiviral Activation Particles (h2) - sc-405985-LAC-2 from Santa Cruz Biotechnology
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ZRANB3 siRNA (h) - sc-94423 from Santa Cruz Biotechnology
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ZRANB3 shRNA Plasmid (h) - sc-94423-SH from Santa Cruz Biotechnology
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ZRANB3 shRNA (h) Lentiviral Particles - sc-94423-V from Santa Cruz Biotechnology
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ZRANB3 RNAi - H00084083-R01 from Novus Biologicals
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MISSION® esiRNA - EHU082921 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN23796 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND23796 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR02662 from Sigma-Aldrich
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ZRANB3 siRNA (Human) - CRJ3295 from Cohesion Biosciences
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ZRANB3 - MBS8200202 from MyBioSource
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shRNA set against Human ZRANB3 (NM_032143.2) - SHH455832 from Creative biogene
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shRNA set against Human ZRANB3(NM_032143.2) - SHL163080 from Creative biogene
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Human ZRANB3 siRNA - orb273796 from Biorbyt
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ZRANB3 shRNA Plasmid (Human) - CHJ3295 from Cohesion Biosciences
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ZRANB3 miRNA 3'UTR clone - MiUTR1H-11756 from Creative biogene
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