NEDD4L

Gene Symbol NEDD4L
Entrez Gene 23327
Alt Symbol NEDD4-2, NEDD4.2, RSP5, hNEDD4-2
Species Human
Gene Type protein-coding
Description neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase NEDD4-like|ubiquitin-protein ligase Rsp5
Swissprots Q7Z5F1 Q9BW58 Q96PU5 Q9NT88 Q7Z5N3 Q3LSM7 Q8WUU9 Q9H2W4 O43165 Q7Z5F2 Q8N5A7
Accessions CAS01775 EAW63064 EAW63065 EAW63066 EAW63067 EAW63068 EAW63069 EAW63070 EAW63071 EAW63072 EAW63073 EAW63074 EAW63075 Q96PU5 AB007899 BAA23711 AB071179 BAB69424 AF070601 AF210730 AAG43524 AF385931 AAM46208 AK091189 AK130420 AK294963 BAH11935 AK300444 BAH13286 AL137469 CAB70754 AY112983 AAM76728 AY112984 AAM76729 AY112985 AAM76730 AY243313 AAP97872 AY243314 AY243315 AY243316 AAP97873 AY243317 AY243318 AY243319 AY243321 AY243322 AY256662 AY312514 AAP75706 AY751751 AAU95503 BC000621 AAH00621 BC019345 AAH19345 BC032597 AAH32597 BP260852 BQ215431 DA248109 DA759940 DA819220 DB317092 DB473368 DC363885 DC381911 DQ181796 ABA10330 DQ890913 ABM81839 DQ893350 ABM84276 XM_005266658 XP_005266715 XM_005266660 XP_005266717 XM_005266663 XP_005266720 XM_006722421 XP_006722484 XM_006722424 XP_006722487 XM_006722425 XP_006722488 XM_006722426 XP_006722489 XM_006722428 XP_006722491 XM_006722430 XP_006722493 XM_011525887 XP_011524189 NM_001144964 NP_001138436 NM_001144965 NP_001138437 NM_001144966 NP_001138438 NM_001144967 NP_001138439 NM_001144968 NP_001138440 NM_001144969 NP_001138441 NM_001144970 NP_001138442 NM_001144971 NP_001138443 NM_001243960 NP_001230889 NM_015277 NP_056092
Function E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. {ECO:0000269|PubMed:12911626, ECO:0000269|PubMed:15040001, ECO:0000269|PubMed:15217910, ECO:0000269|PubMed:15489223, ECO:0000269|PubMed:15496141, ECO:0000269|PubMed:15576372, ECO:0000269|PubMed:19144635}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:15496141, ECO:0000269|PubMed:18819914}. Note=May be recruited to exosomes by NDFIP1.
Tissue Specificity Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. {ECO:0000269|PubMed:14615060, ECO:0000269|PubMed:15496141, ECO:0000269|PubMed:19664597}.
Top Pathways Ubiquitin mediated proteolysis, Endocytosis, Aldosterone-regulated sodium reabsorption

NEDD4L Antibody - 4013 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using NEDD4L Antibody.

Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb - 12146 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D16D6
Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or CIP-treated (+), using Phospho-NEDD4L (Ser342) (D16D6) Rabbit mAb (upper) or NEDD4L Antibody #4013 (lower).

Phospho-NEDD4L (Ser448) Antibody - 8063 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SK-MEL-28 cells, untreated (-) or treated (+) with H 2 O 2 (4 mM, 15 min), using Phospho-NEDD4L (Ser448) Antibody (upper) or NEDD4L Antibody #4013 (lower).

Phospho-NEDD4L (Ser342) Antibody - 4080 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

NEDD4-L (C-12) - sc-390628 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NEDD4-L (C-12): sc-390628. Western blot analysis of NEDD4-L expression in non-transfected: sc-117752 (A) and human NEDD4-L transfected: sc-173451 (B) 293T whole cell lysates.

NEDD4-L (H-138) - sc-366454 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NEDD4-L (H-138): sc-366454. Western blot analysis of NEDD4-L expression in non-transfected: sc-117752 (A) and human NEDD4-L transfected: sc-173451 (B) 293T whole cell lysates.

NEDD4-L (I-17) - sc-85081 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
NEDD4-L (I-17): sc-85081. Western blot analysis of NEDD4-L expression in Jurkat (A) and Caki-1 (B) whole cell lysates.

Anti-NEDD4-2 (phospho S448) antibody [EPR8269(2)] - ab168349 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8269(2)
Detection of NEDD4-2 by Western Blot of Immunprecipitate.293T cell lysate immunoprecipitated using ab168349 at 1/10 dilution. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Anti-NEDD4-2 antibody - ab46521 from Abcam

Prices $398.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NEDD4-2 antibody (ab46521)Lane 1 : Extract from non-transfected HEK293 cellsLane 2 : Extract from HEK293 cells transfected with NEDD4-2

Anti-NEDD4-2 antibody - ab58093 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab58093 NEDD4-2 antibody at 1ug/ml.

Anti-NEDD4-2 antibody - ab124643 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab124643, at 1/250 dilution, staining NEDD4-2 in formalin-fixed, paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry with DAB staining.

Anti-NEDD4-2 antibody - ab172436 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-NEDD4-2 antibody (ab172436) at 1 µg/mlLane 1 : NEDD4L transfected 293T lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.

Anti-NEDD4-2 antibody - ab124125 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NEDD4-2 antibody (ab124125) at 1/100 dilution + mouse brain tissue lysates at 35 µg

Anti-NEDD4-2 antibody [EPR8461] - ab131167 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8461
All lanes : Anti-NEDD4-2 antibody [EPR8461] (ab131167) at 1/10000 dilutionLane 1 : K562 cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit IgG at 1/2000 dilution

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604, APC) - LS-C264178 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604, AP) - LS-C264179 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604, Biotin) - LS-C264180 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604, FITC) - LS-C264181 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604, Azide-free, HRP) - LS-C264182 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604, PE) - LS-C264183 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (C-Terminus, clone EPR8461) - LS-C154494 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR8461
Western blot analysis on (A) K562, (B) 293T, (C) Jurkat, and (D) HeLa cell lysates using anti-NEDD4L antibody.

Anti-NEDD4L / NEDD4-2 Antibody (aa431-480) - LS-C110766 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NEDD4L antibody LS-C110766 Western blot of PANC1 cell lysate.

Anti-NEDD4L / NEDD4-2 Antibody (aa575-604) - LS-C323281 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa425-475) - LS-C289248 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEDD4L / NEDD4-2 Antibody (aa576-604) - LS-C164819 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NEDD4L Antibody western blot of mouse brain tissue lysates (35 ug/lane). The NEDD4L antibody detected the NEDD4L protein (arrow).
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