MIEF1

Gene Symbol MIEF1
Entrez Gene 54471
Alt Symbol HSU79252, MID51, SMCR7L, dJ1104E15.3
Species Human
Gene Type protein-coding
Description mitochondrial elongation factor 1
Other Description SMCR7-like protein|Smith-Magenis syndrome chromosome region, candidate 7-like|mitochondrial dynamic protein MID51|mitochondrial dynamic protein of 51 kDa|mitochondrial dynamics protein MID51|mitochondrial dynamics protein of 51 kDa|smith-Magenis syndrome chromosomal region candidate gene 7 protein-like
Swissprots Q9NQG6 Q9BUI3 Q7L890
Accessions EAW60335 EAW60336 EAW60337 EAW60338 EAW60339 Q9NQG6 AF086364 AK000239 BAA91027 AK026539 AK290954 BAF83643 AL136768 CAB66702 AL365515 CAB97211 AL834205 CAD38892 AM393387 CAL38265 AM393673 CAL38549 AM393747 CAL38622 BC002587 AAH02587 BC002861 BC008327 AAH08327 BC009958 BC032530 BC047292 BC054037 BG419918 DA192893 XM_011530236 XP_011528538 XM_011530237 XP_011528539 XM_011530238 XP_011528540 NM_013298 NM_001304564 NP_001291493 NM_019008 NP_061881 NR_130789 NR_130790
Function Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L. {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:21701560, ECO:0000269|PubMed:23283981, ECO:0000269|PubMed:23530241, ECO:0000269|PubMed:23921378, ECO:0000269|PubMed:24515348}.
Subcellular Location Mitochondrion outer membrane {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:21701560, ECO:0000269|PubMed:24515348}; Single-pass membrane protein {ECO:0000269|PubMed:21508961, ECO:0000269|PubMed:21701560, ECO:0000269|PubMed:24515348}.
Tissue Specificity Expression is relatively high in heart, skeletal muscle, pancreas and kidney. {ECO:0000269|PubMed:21701560}.

SMCR7L (A-6) - sc-514135 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SMCR7L (A-6): sc-514135. Western blot analysis of SMCR7L expression in HeLa (A), MIA PaCa-2 (B) and HT-1080 (C) whole cell lysates.

SMCR7L (P-16) - sc-162237 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SMCR7L (K-18) - sc-86875 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

SMCR7L (G-24) - sc-134014 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal

Anti-SMCR7L antibody - ab199404 from Abcam

Prices $359.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-SMCR7L antibody (ab199404) at 1/500 dilution + Human fetal muscle lysate

Anti-SMCR7L antibody - ab89944 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SMCR7L antibody (ab89944) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-MIEF1 / SMCR7L Antibody (aa112-141, AP) - LS-C241002 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa112-141, APC) - LS-C241004 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa112-141, Biotin) - LS-C241005 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa112-141, FITC) - LS-C241006 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa112-141, PE) - LS-C241007 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa112-141, Azide-free, HRP) - LS-C248201 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa35-84) - LS-C111232 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMCR7L antibody LS-C111232 Western blot of HT1080 cell lysate.

Anti-MIEF1 / SMCR7L Antibody (aa112-141) - LS-C330161 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MIEF1 / SMCR7L Antibody (aa105-132) - LS-C161314 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SMCR7L Antibody western blot of mouse liver tissue lysates (15 ug/lane). The SMCR7L antibody detected SMCR7L protein (arrow).

SMCR7L Antibody - NBP1-59480 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SMCR7L Antibody [NBP1-59480] - Human Fetal Lung.

SMCR7L Antibody - NBP2-13353 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: SMCR7L Antibody [NBP2-13353] - Staining of human stomach, lower shows strong nuclear positivity in glandular cells.

SMCR7L Antibody - NBP1-79453 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SMCR7L Antibody [NBP1-79453] - Human Heart lysate, concentration 0.2-1 ug/ml.

Anti-SMCR7L (231-245) antibody produced in rabbit - SAB1101518 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-SMCR7L (231-245) (RB): <B>Cat. No. SAB1101518</B>: Immunofluorescence of HUVEC cells using Anti-SMCR7L (231-245) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-SMCR7L, (N-terminal) antibody produced in rabbit - SAB2103678 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-SMCR7L, antibody produced in rabbit: <B>Cat. No. SAB2103678</B>: Immunoblotting analysis of SMCR7L in HT1080 cell lysate with SMCR7L anitbody at 1 μg/mL.

Anti-SMCR7L antibody produced in rabbit - HPA019501 from Sigma-Aldrich

Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SMCR7L:<B>Cat. No. HPA019501</B>: Immunohistochemical staining of human stomach, lower shows strong nuclear positivity in glandular cells.

Anti-HSU79252 antibody produced in rabbit - AV51647 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: fetal heart (<B>Cat. No. AV51647</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 0.5 μg/mL in cell type fetal heart

Anti-SMCR7L (1-15) antibody produced in rabbit - SAB1101517 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-SMCR7L (1-15) (RB): <B>Cat. No. SAB1101517</B>: Immunofluorescence of HUVEC cells using Anti-SMCR7L (1-15) (RB) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

SMCR7L antibody - N-terminal region (ARP49422_P050) - ARP49422_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

HSU79252 antibody - C-terminal region (ARP51647_P050) - ARP51647_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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