EIF2D

Gene Symbol EIF2D
Entrez Gene 1939
Alt Symbol HCA56, LGTN
Species Human
Gene Type protein-coding
Description eukaryotic translation initiation factor 2D
Other Description hepatocellular carcinoma-associated antigen 56|ligatin
Swissprots Q9NSN0 Q9NR27 Q8IXV3 Q9NZ21 Q5SY40 Q96TG7 Q96DG3 P41214 Q9NV18
Accessions EAW93536 EAW93537 EAW93538 EAW93539 EAW93540 P41214 AB209364 BAD92601 AF159586 AAD41909 AF220417 AAF34185 AF262403 AAF74205 AK001852 BAA91942 AK225259 AK294536 BAG57743 AL162001 CAB82330 BC001585 AAH01585 BC039134 AAH39134 BC058905 AAH58905 BI465059 DC399556 DQ580499 DQ891590 ABM82516 DQ894784 ABM85710 XM_005277509 XP_005277566 XM_006711196 XP_006711259 XM_011509257 XP_011507559 XM_011509258 XP_011507560 XR_921750 XR_921751 NM_001201478 NP_001188407 NM_006893 NP_008824
Function Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P- site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits. {ECO:0000269|PubMed:20566627, ECO:0000269|PubMed:20713520}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:20566627}.

eIF2D (E-15) - sc-160488 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF2D (E-15): sc-160488. Western blot analysis of eIF2D expression in Jurkat whole cell lysate.

eIF2D (G-14) - sc-160489 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
eIF2D (G-14): sc-160489. Western blot analysis of eIF2D expression in K-562 (A), U-937 (B), Jurkat (C) and HeLa (D) whole cell lysates.

Anti-EIF2D antibody - ab108218 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-EIF2D antibody (ab108218) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-EIF2D antibody - ab86362 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-EIF2D antibody (ab86362) at 1 µg/ml + Transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-EIF2D antibody - ab72794 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-EIF2D antibody (ab72794) at 1/500 dilutionLane 1 : LGTN transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilutiondeveloped using the ECL technique

Anti-LGTN / EIF2D Antibody (N-Terminus) - LS-C345820 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of EIF2D Antibody with human Fetal Liver lysate.

Anti-LGTN / EIF2D Antibody (N-Terminus) - LS-C345819 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of EIF2D Antibody with human HepG2 Whole Cell lysate.

Anti-LGTN / EIF2D Antibody (aa503-552) - LS-C101865 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
EIF2D / LGTN antibody LS-C101865 Western blot of Transfected 293T cell lysate.

Anti-LGTN / EIF2D Antibody (aa350-400) - LS-C289711 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human Ligatin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Ligatin antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Ligatin was also immunoprecipitated by rabbit anti-Ligatin antibody BL10965, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-LGTN / EIF2D Antibody (clone 2D10) - LS-C197112 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D10
Western Blot analysis of EIF2D expression in transfected 293T cell line by EIF2D monoclonal antibody (M05), clone 2D10.Lane 1: EIF2D transfected lysate (Predicted MW: 64.7 KDa).Lane 2: Non-transfected lysate.

LGTN Antibody - NBP1-81308 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LGTN Antibody [NBP1-81308] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416334)

LGTN Antibody - NBP1-57395 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LGTN Antibody [NBP1-57395] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.

LGTN Antibody - NBP1-50038 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: LGTN Antibody [NBP1-50038] - Detection of Human Ligatin by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Ligatin antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Ligatin was also immunoprecipitated by rabbit anti-Ligatin antibody BL10965, which recognizes a downstream epitope. For blotting immunoprecipitated Ligatin, the ReliaBLOT(R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

LGTN Antibody - H00001939-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: LGTN Antibody [H00001939-B01] - analysis of LGTN expression in transfected 293T cell line by LGTN MaxPab polyclonal antibody (B01). Lane 1: LGTN transfected lysate (64.70 KDa). Lane 2: Non-transfected lysate.

LGTN Antibody (2D10) - H00001939-M05 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D10
Western Blot: LGTN Antibody (2D10) [H00001939-M05] - Analysis of EIF2D expression in human liver.

LGTN Antibody - H00001939-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-LGTN (226-240) antibody produced in rabbit - SAB1101117 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-LGTN (226-240) (RB): <B>Cat. No. SAB1101117</B>: Immunofluorescence of HUVEC cells using LGTN (226-240) (RB), Cat. No. SAB1101117 (red) at a 1:200 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-LGTN antibody produced in rabbit - SAB2103019 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LGTN, antibody produced in rabbit: <B>Cat. No. SAB2103019</B>: Immunoblotting analysis of LGTN in transfected 293T cell lysate with LGTN anitbody at 1 μg/mL.

Anti-LGTN (371-385) antibody produced in rabbit - SAB1101118 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-LGTN (371-385) (RB): <B>Cat. No. SAB1101118</B>: Immunofluorescence of HUVEC cells using LGTN (371-385) (RB), Cat. No. SAB1101118 (red) at a 1:200 dilution, taken at 40x magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-EIF2D antibody produced in rabbit - HPA028220 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-LGTN: <B>Cat. No. HPA028220</B>: Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Monoclonal Anti-LGTN, (C-terminal) antibody produced in mouse - SAB1402760 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2D10
<B>Immunoblotting</B><BR/>Monoclonal Anti-LGTN: <B>Cat. No. SAB1402760</B>: Antibody concentration: 1 μg/mL. Specific band of ~37.1 kDa using immunogen protein lysate.

Anti-LGTN antibody produced in mouse - SAB1408963 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-LGTN: <B>Cat. No. SAB1408963</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~64.2 kDa using EIF2D transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

LGTN antibody - middle region (ARP40690_P050) - ARP40690_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

EIF2D Antibody - N-terminal region (ARP74930_P050) - ARP74930_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

EIF2D Antibody - N-terminal region (ARP74931_P050) - ARP74931_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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