MAP2K4

Gene Symbol MAP2K4
Entrez Gene 6416
Alt Symbol JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1
Species Human
Gene Type protein-coding
Description mitogen-activated protein kinase kinase 4
Other Description JNK-activated kinase 1|JNK-activating kinase 1|MAP kinase kinase 4|MAPK/ERK kinase 4|MAPKK 4|MEK 4|SAPK/ERK kinase 1|c-Jun N-terminal kinase kinase 1|dual specificity mitogen-activated protein kinase kinase 4|stress-activated protein kinase kinase 1
Swissprots Q6FHX4 Q5U0B8 P45985 B2R7N7 Q6PIE6 D3DTS5 Q6P9H2 B3KYB2
Accessions AAC24130 AAY22176 EAW89973 EAW89974 EAW89975 EAW89976 P45985 AA293365 AK122997 AK131544 BAG54774 AK294923 BAG58005 AK313053 BAG35884 AK316100 BAH14471 AK316177 BAH14548 AU150695 BC036032 AAH36032 BC050386 BC060764 AAH60764 BQ028457 BT019676 AAV38482 CR536564 CAG38801 L31902 L36870 AAC41719 U17743 AAC50127 XM_005256753 XP_005256810 XM_005256755 XP_005256812 XM_011523976 XP_011522278 XM_011523977 XP_011522279 XR_934080 NM_001281435 NP_001268364 NM_003010 NP_003001
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/J
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
Tissue Specificity Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Top Pathways TNF signaling pathway, Toll-like receptor signaling pathway, Epstein-Barr virus infection, Influenza A, HTLV-I infection

Phospho-SEK1/MKK4 (Thr261) Antibody - 9151 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from untreated and UV treated 3T3, C6 and 293 cells, using Phospho-SEK1/MKK4 (Thr261) Antibody.

Phospho-SEK1/MKK4 (Ser80) Antibody - 9155 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated (lane 1), PDGF-treated (lane 2) or stably transfected with Src (lane 3), using Phospho-SEK1/MKK4 (Ser80) Antibody (upper) or SEK1/MKK4 Antibody #9152 (lower).

Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody - 9156 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa and C6 cells, untreated or UV-treated, using Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody (upper) or SEK1/MKK4 Antibody #9152 (lower).

Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb - 4514 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C36C11
Western blot analysis of extracts from various cell lines, untreated or treated with sorbitol or UV, as indicated, using Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb.

SEK1 (5C10) Rabbit mAb - 3346 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 5C10
Western blot analysis of extracts from HeLa, NIH/3T3 and COS cells, using SEK1 (5C10) Rabbit mAb.

SEK1/MKK4 Antibody - 9152 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HEK293, NIH/3T3, C6, COS and A431 cells, using SEK1/MKK4 Antibody.

MEK-4 (G-7) - sc-376838 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-4 (G-7): sc-376838. Western blot analysis of MEK-4 expression in Src-3T3 whole cell lysate.

MEK-4 (K-18) - sc-964 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of MEK-4 expression in NIH/3T3 (A,C) and Src-3T3 (B,D) whole cell lysates. Antibodies tested include MEK-4 (K-18): sc-964 (A,B) and MEK-4 (C-20): sc-837 (C,D).

MEK-4 (C-20) - sc-837 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-4 (C-20): sc-837. Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic and membrane staining.

MEK-4 (G-6) - sc-166168 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-4 (G-6): sc-166168. Western blot analysis of MEK-4 expression in NIH/3T3 (A) and Src-3T3 (B) whole cell lysates.

MEK-4 (H-98) - sc-13070 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
MEK-4 (H-98): sc-13070. Western blot analysis of MEK-4 expression in NIH/3T3 whole cell lysate.

MEK-4 (54) - sc-136314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MEK-4 (54): sc-136314. Western blot analysis of MEK-4 expression in rat brain tissue extract.

p-MEK-4 (Thr 261) - sc-7990 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-MEK-4 (Thr 261)-R: sc-7990-R. Western blot analysis of MEK-4 phosphorylation in untreated (A), UV irradiated (B) and lambda protein phosphatase (sc-200312A) treated and UV irradiated (C) HEK293 whole cell lysates.

p-MEK-4 (Thr 261) - sc-101795 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-MEK-4 (Thr 261): sc-101795. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining.

p-MEK-4 (Ser 80) - sc-101794 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-MEK-4 (Ser 80): sc-101794. Western blot analysis of phosphorylated MEK-4 expression in untreated (A) and UV-treated (B) 293 whole cell lysates.

Anti-MEK4 (phospho S257 + T261) antibody - ab4760 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-MEK4 (phospho S257) antibody - ab61112 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61112, at a 1/50 dilution, staining MEK4 in paraffin embedded human brain tissue by Immunohistochemistry with (+) or without (-) the immunising phospopeptide.

Anti-MEK4 (phospho S80) antibody - ab39403 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab39403, at a 1/500 dilution, staining human SEK1 / MKK4 in breast carcinoma tissue by Immunohistochemistry, Paraffin embedded tissue.

Anti-MEK4 (phospho S80) antibody - ab131353 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol fixed HeLa cells labelling MEK4 (phospho S80) with ab131353 at 1/100 dilution.

Anti-MEK4 (phospho S80) antibody - ab194811 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical staining of Human breast carcinoma tissue labeling MEK4 (phospho S80) using ab194811 at 1/50 dilution.

Anti-MEK4 (phospho S80) antibody - ab118633 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118633 at 10 µg/ml staining MEK4 (phospho S80) in Formalin-Fixed, Paraffin-Embedded Human Kidney tissue followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen, by Immunohistochemistry.

Anti-MEK4 (phospho S80) antibody [EP1075Y] - ab52958 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1075Y
All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilutionLane 1 : 3T3 cell lysate untreatedLane 2 : 3T3 cell lysate treated with PDGFLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-MEK4 (phospho T261) antibody - ab131351 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol fixed HeLa cells labelling MKK4 (phospho T261) with ab131351 antibody at 1/100 dilution.

Anti-MEK4 (phospho T261) antibody - ab194820 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of methanol-fixed HeLa cells labeling MEK4 using ab194820 at 1/100 diluiton.

Anti-MEK4 (phospho T261) antibody - ab192227 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MEK4 (phospho T261) antibody (ab192227) at 1/500 dilutionLane 1 : UV treated 293 whole cell lysate with antigen-specific peptideLane 2 : UV treated 293 whole cell lysateLysates/proteins at 12.5 µg per lane.
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