ECT2

Gene Symbol	ECT2
Entrez Gene	1894
Alt Symbol	ARHGEF31
Species	Human
Gene Type	protein-coding
Description	epithelial cell transforming 2
Other Description	epithelial cell transforming sequence 2 oncogene\|epithelial cell-transforming sequence 2 oncogene\|protein ECT2
Swissprots	Q6U836 Q9H8V3 Q9NVW9 Q2M269 Q0MT80 Q9NSV8
Accessions	CAM84950 CCA61729 CCA64133 CCA64578 CCA64581 CCB07385 CCQ77688 CCQ77697 CCQ77706 CCQ77715 CDH48194 CDH61616 CDH61670 CDH61689 CDH88987 CDH92525 CDH92539 CDH92550 CDH92797 CDH92894 CDI44669 CDI44678 EAW78460 EAW78461 Q9H8V3 AK001323 BAA91624 AK001515 AK023267 BAB14498 AK027713 BAB55317 AK308391 AK314581 BAG37157 AL137710 CAB70886 AY376439 AAQ83675 BC006838 AAH06838 BC006987 BC070038 BC112086 AAI12087 DB229273 DC406772 DQ847274 ABH10140 XM_005247176 XP_005247233 XM_006713523 XP_006713586 XM_006713524 XP_006713587 XM_011512514 XP_011510816 XM_011512515 XP_011510817 XM_011512516 XP_011510818 NM_001258315 NP_001245244 NM_001258316 NP_001245245 NM_018098 NP_060568
Function	Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth
Subcellular Location	Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
Tissue Specificity	Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. {ECO:0000269\|PubMed:19617897}.

Ect2 (C-20) - sc-1005 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Ect2 (C-20): sc-1005. Western blot analysis of Ect2 expression in NIH/3T3 whole cell lysate.

Ect2 (H-300) - sc-25637 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

Ect2 (H-300): sc-25637. Western blot analysis of Ect2 expression in non-transfected: sc-110760 (A) and human Ect2 transfected: sc-110479 (B) 293 whole cell lysates.

Anti-ECT2 antibody - ab4863 from Abcam

Prices	$378.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

This image was kindly supplied as part of the review submitted by Naslin Rasheed.MEF cells were incubated with ab4863 (1/500) for 2 hours at room temperature. The staining shows the protein is localised to the nucleus.

Anti-ECT2 antibody - ab123571 from Abcam

Prices	$376.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 antibody (ab123571) at 1/100 dilution + 293 cell lysate at 35 µg

Anti-ECT2 antibody - ab86604 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ECT2 antibody (ab86604) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

Anti-ECT2 antibody - N-terminal - ab151503 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling ECT2 with ab151503 at 1/100 dilution.

Anti-ECT2 Antibody (aa2-14) - LS-C54498 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Goat
Clonality	Polyclonal

Anti-ECT2 Antibody (aa597-625, APC) - LS-C234848 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (phospho-Thr790) - LS-C19111 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 pT790 Antibody - Western Blot. Western blot of affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000. Personal Communication, Toru Miki, CCR-NCI, Bethesda, MD.

Anti-ECT2 Antibody (aa597-625, Biotin) - LS-C234849 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa597-625, AP) - LS-C234850 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa597-625, FITC) - LS-C234851 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa551-600) - LS-C81717 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Hela cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ECT2 Antibody (aa580-629) - LS-C81718 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ECT2 Antibody (aa597-625, Azide-free, HRP) - LS-C249888 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa597-625, PE) - LS-C234852 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa453-647, Biotin) - LS-C300087 from LifeSpan Bioscience

Prices	$425.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa166-348, Biotin) - LS-C297100 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa453-647, FITC) - LS-C304650 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa166-348, FITC) - LS-C303856 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa597-625) - LS-C167617 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

ECT2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung squamous carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-ECT2 Antibody (aa833-883) - LS-C289095 from LifeSpan Bioscience

Prices	$375.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Detection of Human ECT2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and 293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-ECT2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-ECT2 Antibody (aa166-348) - LS-C294137 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa565-594) - LS-C321012 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-ECT2 Antibody (aa453-647) - LS-C294138 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

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