RAG1

Gene Symbol	RAG1
Entrez Gene	5896
Alt Symbol	RAG-1, RNF74
Species	Human
Gene Type	protein-coding
Description	recombination activating gene 1
Other Description	RING finger protein 74|V(D)J recombination-activating protein 1|recombination activating protein 1
Swissprots	Q8IY72 Q8NER2 E9PPC4 P15918
Accessions	AAG38421 AAM77798 CBX87169 EAW68118 P15918 AY235716 BC037344 AAH37344 M29474 AAA60248 XM_005253041 XP_005253098 XM_011520250 XP_011518552 XM_011520251 XP_011518553 NM_000448 NP_000439
Function	Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'- hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt,
Subcellular Location	Nucleus {ECO:0000255|PROSITE- ProRule:PRU00820}.
Tissue Specificity	Maturing lymphoid cells.
Top Pathways	Primary immunodeficiency

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

RAG-1 CRISPR/Cas9 KO Plasmid (h) - sc-401821 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 HDR Plasmid (h) - sc-401821-HDR from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 Double Nickase Plasmid (h) - sc-401821-NIC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 Double Nickase Plasmid (h2) - sc-401821-NIC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 CRISPR Activation Plasmid (h) - sc-401821-ACT from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 CRISPR Activation Plasmid (h2) - sc-401821-ACT-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 Lentiviral Activation Particles (h) - sc-401821-LAC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 Lentiviral Activation Particles (h2) - sc-401821-LAC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

RAG-1 siRNA (h) - sc-42962 from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

RAG-1 shRNA Plasmid (h) - sc-42962-SH from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

RAG-1 shRNA (h) Lentiviral Particles - sc-42962-V from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

RAG1 RNAi - H00005896-R01 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

MISSION® esiRNA - EHU142631 from Sigma-Aldrich Category siRNA Prices $250.00 Sizes 50 µg

MISSION® esiRNA - EHU100611 from Sigma-Aldrich Category siRNA Prices $250.00 Sizes 50 µg

CompoZr® Knockout ZFN Kit - CKOZFN17915 from Sigma-Aldrich Category ZFN Kit Prices $4,999.00 Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17915 from Sigma-Aldrich Category ZFN Kit Prices $3,999.00 Sizes 1 kt

RAG1 siRNA (Human) - CRH3984 from Cohesion Biosciences Category siRNA Prices $340.00, $510.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

RAG1 - MBS8200363 from MyBioSource Prices $365.00, $515.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

shRNA set against Human RAG1 (NM_000448.2) - SHH389212 from Creative biogene Category siRNA Species Reactivities Human

Human RAG1 siRNA - orb264486 from Biorbyt Category siRNA Prices $485.00, $629.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

RAG1 shRNA Plasmid (Human) - CHH3984 from Cohesion Biosciences Category siRNA Prices $500.00, $750.00 Sizes 150 µg, 300 µg Species Reactivities Human

RAG1 miRNA 3'UTR clone - MiUTR4H-TG07303 from Creative biogene Species Reactivities Human

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