PLK3
| Gene Symbol | PLK3 |
|---|---|
| Entrez Gene | 1263 |
| Alt Symbol | CNK, FNK, PRK |
| Species | Human |
| Gene Type | protein-coding |
| Description | polo-like kinase 3 |
| Other Description | FGF-inducible kinase|PLK-3|cytokine-inducible kinase|cytokine-inducible serine/threonine-protein kinase|proliferation-related kinase|serine/threonine-protein kinase PLK3 |
| Swissprots | Q9H4B4 Q5JR99 Q15767 Q96CV1 |
| Accessions | AAU88146 EAX07021 Q9H4B4 AA421212 AB209875 BAD93112 AJ293866 CAC10659 BC004135 BC004198 BC013899 AAH13899 BC013960 EU832631 ACE87722 EU832706 ACE87038 U56998 AAC50637 XR_246234 XR_946541 NM_004073 NP_004064 |
| Function | Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 i |
| Subcellular Location | Cytoplasm. Nucleus. Nucleus, nucleolus. Golgi apparatus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Translocates to the nucleus upon cisplatin treatment. Localizes to the Golgi apparatus during interphase. According to a report, PLK3 localizes only in the nucleolus and not in the centrosome, or in any other location in the cytoplasm (PubMed:17264206). The discrepancies in results may be explained by the PLK3 antibody specificity, by cell line- specific expression or post-translational modifications. {ECO:0000269|PubMed:17264206}. |
| Tissue Specificity | Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. May have a short half-live. In cells of hematopoietic origin, strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. |
| Top Pathways | Tuberculosis |
Fnk CRISPR/Cas9 KO Plasmid (h) - sc-404710 from Santa Cruz Biotechnology
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Fnk HDR Plasmid (h) - sc-404710-HDR from Santa Cruz Biotechnology
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Fnk Double Nickase Plasmid (h) - sc-404710-NIC from Santa Cruz Biotechnology
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Fnk Double Nickase Plasmid (h2) - sc-404710-NIC-2 from Santa Cruz Biotechnology
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Fnk CRISPR Activation Plasmid (h) - sc-404710-ACT from Santa Cruz Biotechnology
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Fnk CRISPR Activation Plasmid (h2) - sc-404710-ACT-2 from Santa Cruz Biotechnology
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Fnk Lentiviral Activation Particles (h) - sc-404710-LAC from Santa Cruz Biotechnology
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Fnk Lentiviral Activation Particles (h2) - sc-404710-LAC-2 from Santa Cruz Biotechnology
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Fnk siRNA (h) - sc-39150 from Santa Cruz Biotechnology
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Fnk shRNA Plasmid (h) - sc-39150-SH from Santa Cruz Biotechnology
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Fnk shRNA (h) Lentiviral Particles - sc-39150-V from Santa Cruz Biotechnology
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PLK3 RNAi - H00001263-R02 from Novus Biologicals
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MISSION® esiRNA - EHU149801 from Sigma-Aldrich
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MISSION® siRNA - SIHK1747 from Sigma-Aldrich
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MISSION® siRNA - SIHK1749 from Sigma-Aldrich
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MISSION® siRNA - SIHK1748 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN16988 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16988 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR02208 from Sigma-Aldrich
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PLK3 siRNA (Human) - CRH0883 from Cohesion Biosciences
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Fnk CRISPR/Cas9 KO Plasmid (h2) - sc-404710-KO-2 from Santa Cruz Biotechnology
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Fnk HDR Plasmid (h2) - sc-404710-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human PLK3 (NM_004073.2) - SHH377916 from Creative biogene
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shRNA set against Human PLK3(NM_004073.2) - SHR122688 from Creative biogene
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PLK3 shRNA Plasmid (Human) - CHH0883 from Cohesion Biosciences
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