Gene Symbol | ALKBH2 |
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Entrez Gene | 121642 |
Alt Symbol | ABH2 |
Species | Human |
Gene Type | protein-coding |
Description | alkB, alkylation repair homolog 2 (E. coli) |
Other Description | 2OG-Fe(II) oxy DC1|DNA oxidative demethylase ALKBH2|alkylated DNA repair protein alkB homolog 2|alpha-ketoglutarate-dependent dioxygenase alkB homolog 2|oxy DC1 |
Swissprots | Q6NS38 A4PET2 Q5XLE3 |
Accessions | CAG17897 CCQ43124 EAW97845 Q6NS38 AB277859 BAF56576 AI278638 AK309062 AY754389 AAV28301 BC070489 AAH70489 BM785502 BM790974 BM820131 BU623121 CN421655 DA182735 HQ447536 ADQ32022 XM_005253835 XP_005253892 XM_005253836 XP_005253893 NM_001001655 NP_001001655 NM_001145374 NP_001138846 NM_001145375 NP_001138847 NM_001205179 NP_001192108 NM_001205180 NP_001192109 |
Function | Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517, ECO:0000269|PubMed:16174769, ECO:0000269|PubMed:18432238, ECO:0000269|PubMed:18519673}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:12594517}. Note=Detected in replication foci during S-phase. |
Tissue Specificity | Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder. {ECO:0000269|PubMed:12486230, ECO:0000269|PubMed:16174769}. |
ALKBH2 CRISPR/Cas9 KO Plasmid (h) - sc-407363 from Santa Cruz Biotechnology
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ALKBH2 HDR Plasmid (h) - sc-407363-HDR from Santa Cruz Biotechnology
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ALKBH2 Double Nickase Plasmid (h) - sc-407363-NIC from Santa Cruz Biotechnology
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ALKBH2 Double Nickase Plasmid (h2) - sc-407363-NIC-2 from Santa Cruz Biotechnology
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ALKBH2 CRISPR Activation Plasmid (h) - sc-407363-ACT from Santa Cruz Biotechnology
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ALKBH2 CRISPR Activation Plasmid (h2) - sc-407363-ACT-2 from Santa Cruz Biotechnology
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ALKBH2 Lentiviral Activation Particles (h) - sc-407363-LAC from Santa Cruz Biotechnology
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ALKBH2 Lentiviral Activation Particles (h2) - sc-407363-LAC-2 from Santa Cruz Biotechnology
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ALKBH2 siRNA (h) - sc-96063 from Santa Cruz Biotechnology
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ALKBH2 shRNA Plasmid (h) - sc-96063-SH from Santa Cruz Biotechnology
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ALKBH2 shRNA (h) Lentiviral Particles - sc-96063-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN3067 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3067 from Sigma-Aldrich
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ALKBH2 siRNA (Human) - CRJ4683 from Cohesion Biosciences
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ALKBH2 - MBS8232801 from MyBioSource
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shRNA set against Human ALKBH2 (NM_001001655.2) - SHH235222 from Creative biogene
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Human ALKBH2 siRNA - orb275184 from Biorbyt
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ALKBH2 shRNA Plasmid (Human) - CHJ4683 from Cohesion Biosciences
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ALKBH2 miRNA 3'UTR clone - MiUTR4H-TG00277 from Creative biogene
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