MYL12A

Gene Symbol MYL12A
Entrez Gene 10627
Alt Symbol HEL-S-24, MLC-2B, MLCB, MRCL3, MRLC3, MYL2B
Species Human
Gene Type protein-coding
Description myosin, light chain 12A, regulatory, non-sarcomeric
Other Description epididymis secretory protein Li 24|myosin RLC|myosin regulatory light chain 12A|myosin regulatory light chain 2, nonsarcomeric|myosin regulatory light chain 3|myosin regulatory light chain MRLC3|myosin, light polypeptide, regulatory, non-sarcomeric (20kD)
Swissprots D3DUH6 Q13182 Q7Z5Z4 Q53X45 P19105 O14950
Accessions EAX01678 EAX01679 EAX01680 EAX01681 P19105 AK222569 BAD96289 AK291145 BAF83834 AW958488 BC016372 AAH16372 BC031972 AAH31972 BC032748 AAH32748 BM561281 BU615599 CA309747 CD702288 D82059 BAB88919 DQ185044 ABD14424 DQ892814 ABM83740 DQ896060 ABM87059 EU794621 ACJ13675 X54304 CAA38201 XM_005258078 XP_005258135 NM_001303047 NP_001289976 NM_001303048 NP_001289977 NM_001303049 NP_001289978 NM_006471 NP_006462
Function Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Phosphorylation triggers actin polymerization in vascular smooth muscle. Implicated in cytokinesis, receptor capping, and cell locomotion (By similarity). {ECO:0000250}.
Tissue Specificity Ubiquitously expressed in various hematopoietic cells. {ECO:0000269|PubMed:11436981, ECO:0000269|PubMed:18480596}.
Top Pathways Tight junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton

MRCL3/MRLC2 (A-10) - sc-376606 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MRCL3/MRLC2 (A-10): sc-376606. Western blot analysis of MRCL3/MRLC2 expression in BC3H1 (A) and A-10 (B) whole cell lysates.

MRCL3/MRLC2 (A-20) - sc-9449 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
MLC (A-20): sc-9449. Immunofluorescence staining of methanol-fixed A10 cells showing cytoplasmic localization.

MRCL3/MRLC2 (G-5) - sc-376677 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
MRCL3/MRLC2 (G-5): sc-376677. Western blot analysis of MRCL3/MRLC2 expression in BC3H1 (A) and A-10 (B) whole cell lysates.

Anti-MRCL3 (phospho S19) antibody - ab194918 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling MRCL3 (phospho S18) with ab194918 at 1/50 dilution.

Anti-MRCL3 (phospho T17 + S18) antibody [EPR1457] - ab126739 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1457
All lanes : Anti-MRCL3 (phospho T17 + S18) antibody [EPR1457] (ab126739) at 1/1000 dilutionLane 1 : NIH/3T3 cell lysateLane 2 : NIH/3T3 cell lysate + Calyculin ALysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-MRCL3 antibody - ab62377 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MRCL3 antibody (ab62377) at 1/5000 dilution + 293T cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP conjugate at 1/2000 dilution

Anti-MRCL3 antibody - ab197170 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human fetal kidney tissue labeling MRCL3 with ab197170 at 1/100 dilution.

Anti-MRCL3 antibody [EP1456Y] - ab81273 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1456Y
Anti-MRCL3 antibody [EP1456Y] (ab81273) at 1/1000 dilution + 293T cell lysate at 10 µgSecondaryGoat anti-rabbit HRP labelled IgG at 1/2000 dilution

Anti-MRCL3 antibody - ab131530 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MRCL3 antibody (ab131530) at 1/500 dilutionLane 1 : K562 cell extract with blocking peptideLane 2 : K562 cell extract

Anti-MYL12A / MRCL3 Antibody (phospho-Ser19) IHC-plus™ - LS-B67 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-MYL9 antibody IHC staining of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B67 concentration 5 ug/ml.

Anti-MYL12A / MRCL3 Antibody (phospho-Ser18) - LS-C291150 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MYL12A / MRCL3 Antibody (aa10-171, Biotin) - LS-C300532 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MYL12A / MRCL3 Antibody (aa10-171, FITC) - LS-C303506 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MYL12A / MRCL3 Antibody (aa10-171) - LS-C295509 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant MYL12A / MRCL3.

Anti-MYL12A / MRCL3 Antibody (phospho-Ser18) - LS-C336045 from LifeSpan Bioscience

Prices $285.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-MYL12A-S18 antibody.

Anti-MYL12A / MRCL3 Antibody (N-Terminus, clone EP1456Y) - LS-C105273 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1456Y
Western blot analysis on 293T cell lysate using anti-Myosin RLC antibody (LS-C105273), dilution 1:1000.

Anti-Myosin RLC Antibody (aa12-27) - LS-C56830 from LifeSpan Bioscience

Prices $440.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-Myosin RLC Antibody (aa12-27) - LS-C122187 from LifeSpan Bioscience

Prices $650.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-MLC2 / MYL9 Antibody (aa95-125) - LS-C329862 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Myosin RLC Antibody (phospho-Thr17/Ser18, clone EPR1457) - LS-C152258 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1457
Western blot analysis on NIH/3T3 cell lysates using anti-Phospho-Myosin RLC (pT17/pS18) antibody. Cells were either (A) untreated or (B) treated with Calyculin A.

MRCL3 [p Ser19, p Ser20] Antibody - NB100-1687 from Novus Biologicals

Prices $369.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western Blot: MRCL3 [p Ser19, p Ser20] Antibody [NB100-1687] - Used at a 1:1000 dilution to detect myosin light chain by Western blot on 3T3 cell lysates. A standard urea/glycerol gel without SDS was used to separate phospho forms of regulatory light chain according to mass to charge ratios. In Panel A, reactivity of phosphospecific antibody is shown. In Panel B, reactivity of commercially available pan reactive antibody that detects both unphosphorylated and phosphorylated forms of regulatory light chain is shown. phosphospecific antibody detects both monophosphorylated (pSer20 Mono-P-RLC) and diphosphorylated (pThr19-pSer20 Di-P-RLC) regulatory light chain.

Anti-MRCL3 (1-13) antibody produced in rabbit - SAB1101690 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MRCL3 (1-13) (RB): <B>Cat. No. SAB1101690</B>: Immunofluorescence of HUVEC cells using Anti-MRCL3 (1-13) (RB) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-MRCL3 (Ab-18) antibody produced in rabbit - SAB4300424 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-MRCL3 (Ab-18): <B>SAB4300424</B>: Staining of methanol-fixed HeLa cells using Anti-MRCL3 (Ab-18) antibody (Red).

Anti-phospho-MRCL3 (pSer18) antibody produced in rabbit - SAB4300093 from Sigma-Aldrich

Prices $397.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-phospho-MRCL3 (pSer18): <B>SAB4300093</B>: Analysis of paraffin-embedded human breast carcinoma tissue using Anti-phospho-MRCL3 (pSer18) antibody (left) or the same antibody preincubated with blocking peptide (right).

Myosin-RLC-Antibody-N-term - AJ1522b from Abgent, a WuXi AppTec company

Host Rabbit
Clonality Monoclonal
Clone EP1456Y
A. Western blot analysis on 293T cell lysate using anti-Myosin RLC RabMAb (Cat. #AJ1522b), dilution 1:1000.
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