RFWD3

Gene Symbol RFWD3
Entrez Gene 55159
Alt Symbol RNF201
Species Human
Gene Type protein-coding
Description ring finger and WD repeat domain 3
Other Description E3 ubiquitin-protein ligase RFWD3|RING finger and WD repeat domain-containing protein 3|RING finger protein 201
Swissprots Q5XKR3 D3DUJ8 A8K585 Q9H9Q3 Q9NVT4 B2RE35 Q6PCD5
Accessions EAW95680 EAW95681 Q6PCD5 AK001382 BAA91662 AK022673 BAB14169 AK291200 BAF83889 AK296727 BAG59315 AK315786 BAG38132 BC002574 AAH02574 BC059371 AAH59371 BM467919 BP872110 XM_005256021 XP_005256078 XM_005256022 XP_005256079 XM_006721228 XP_006721291 XM_011523191 XP_011521493 NM_018124 NP_060594
Function E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. Plays a role in RPA-mediated DNA damage signaling and repair. {ECO:0000269|PubMed:20173098, ECO:0000269|PubMed:21504906, ECO:0000269|PubMed:21558276}.
Subcellular Location Nucleus. Nucleus, PML body. Cytoplasm. Note=In undamaged cells, found both in the cytoplasm and in the nucleus, partially associated with PML nuclear bodies. In response to replication block, such as that caused by hydroxyurea treatment, or to DNA damage caused by ionizing radiations or doxorubicin, recruited to the nucleus, to stalled replication forks or to sites of DNA repair. This recruitment depends upon RPA2.

RFWD3 (C-18) - sc-243976 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RFWD3 (L-17) - sc-243975 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

RFWD3 (T-19) - sc-243974 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RFWD3 (T-19): sc-243974. Western blot analysis of RFWD3 expression in Jurkat whole cell lysate.

Anti-RFWD3 antibody - ab138030 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-RFWD3 antibody (ab138030) at 1/500 dilution + HepG2 cell lysate at 30 µg

Anti-RFWD3 antibody - ab99306 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
An anti RFWD3 antibody, at 1 µg/ml, staining RFWD3 in HeLa whole cell lysate immunoprecipitated using ab99306 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-RFWD3 Antibody (aa75-125) - LS-C288188 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD3 Antibody (aa175-225) - LS-C288190 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD3 Antibody (aa724-774) - LS-C288192 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD3 Antibody (aa358-407) - LS-C200591 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HepG2 cells using RFWD3 antibody.

Anti-RFWD3 Antibody (aa374-423) - LS-C120343 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from MCF-7 cells, using RFWD3 Antibody. The lane on the right is treated with the synthesized peptide.

RFWD3 Antibody - NB100-68206 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RFWD3 Antibody (6B4) - H00055159-M01 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6B4

RFWD3 Antibody - NB100-68208 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RFWD3 Antibody [NB100-68208] - Detection of Human RFWD3 on HeLa whole cell lysate using NB100-68208. RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207.

RFWD3 Antibody - NBP2-20141 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RFWD3 Antibody [NBP2-20141] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:3000.

RFWD3 Antibody - NB100-68207 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RFWD3 Antibody - H00055159-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

Anti-RFWD3 antibody produced in rabbit - SAB4502299 from Sigma-Aldrich

Prices $335.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RFWD3: <B>Cat. No. SAB4502299</B>: Immunoblotting analysis of extracts from MCF-7 cells, using Anti-RFWD3 antibody. The lane on the left was treated with the Anti-RFWD3 antibody. The lane on the right (negative control) was treated with both Anti-RFWD3 antibody and the synthesized immunogen peptide.

Monoclonal Anti-RFWD3 antibody produced in mouse - WH0055159M1 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 6B4
<B>Immunoblotting</B><BR/>Monoclonal Anti-RFWD3, <B>Cat. No. WH0055159M1</B> used at the antibody concentration: 1 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.

Anti-RFWD3 (191-205) antibody produced in rabbit - R2906 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-RFWD3 (191-205): <B>Cat. No. R2906</B>: Immunofluorescence of HUVEC cells using RFWD3 (191-205) (RB),<B>Cat. No. R2906</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

RFWD3 Antibody (OAAF04209) - OAAF04209 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RFWD3 Antibody (OAAF07037) - OAAF07037 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

RFWD3 antibody - 70R-19864 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

RFWD3 Antibody - abx014835 from Abbexa

Host Rabbit
Clonality Polyclonal

RFWD3 / RNF201 antibody - 19893-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical of paraffin-embedded human testis using 19893-1-AP(RFWD3 antibody) at dilution of 1:400 (under 10x lens)

RFWD3 / RNF201 antibody - H00055159-A01 from Acris Antibodies

Prices $300.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
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