AMBRA1

Gene Symbol AMBRA1
Entrez Gene 55626
Alt Symbol DCAF3, WDR94
Species Human
Gene Type protein-coding
Description autophagy/beclin-1 regulator 1
Other Description DDB1 and CUL4 associated factor 3|WD repeat domain 94|activating molecule in BECN1-regulated autophagy protein 1|activating molecule in beclin-1-regulated autophagy
Swissprots Q9NXE7 G3V193 A6XN33 Q86XD6 Q9C0C7 D3DQP8 Q9H8Z0
Accessions EAW67996 EAW67997 EAW67998 EAW67999 EAW68000 EAW68001 EAW68002 Q9C0C7 AB051523 BAB21827 AK000301 BAA91067 AK023197 BAB14457 AK057896 AK302808 BAG64009 AK304530 BAG65331 AL137611 AL834190 BC045609 AAH45609 BF589843 CA389399 DB070055 DB232750 DQ870924 ABI74670 XM_005253009 XP_005253066 XM_005253011 XP_005253068 XM_005253014 XP_005253071 XM_006718259 XP_006718322 XM_006718260 XP_006718323 XM_011520214 XP_011518516 XM_011520215 XP_011518517 XR_930878 NM_001267782 NP_001254711 NM_001267783 NP_001254712 NM_001300731 NP_001287660 NM_017749 NP_060219
Function Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity). {ECO:0000250}.
Subcellular Location Cytoplasmic vesicle, autophagosome {ECO:0000250}. Note=Localizes also to discrete punctae along the ciliary axoneme. {ECO:0000250}.

Ambra1 Antibody - 12250 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Ambra1 Antibody.

AMBRA1 (G-6) - sc-398204 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AMBRA1 (G-6): sc-398204. Western blot analysis of AMBRA1 expression in HeLa whole cell lysate.

AMBRA1 (H-300) - sc-366376 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AMBRA1 (H-300): sc-366376. Western blot analysis of AMBRA1 expression in Hep G2 whole cell lysate.

AMBRA1 (N-16) - sc-130091 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

AMBRA1 (N-18) - sc-130116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
AMBRA1 (N-18): sc-130116. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.

AMBRA1 (Q-15) - sc-138096 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 antibody - ab69501 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-AMBRA1 antibody (ab69501) at 1 µg/ml + 3T3 cell lysate at 15 µgSecondaryAnti-rabbit

Anti-AMBRA1 antibody - ab69503 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-AMBRA1 antibody (ab69503) at 2 µg/ml + mouse brain tissue lysate at 15 µgSecondaryAnti-rabbit

Anti-AMBRA1 antibody - ab81022 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 antibody - ab59141 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab59141 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59141, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-AMBRA1 antibody - ab72098 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue labelling AMBRA1 with ab72098 at 10µg/ml. Staining was enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Anti-AMBRA1 Antibody (Internal) - LS-C93920 from LifeSpan Bioscience

Prices $325.00
Sizes 500 µg
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (N-Terminus) - LS-C144689 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody - LS-C150716 from LifeSpan Bioscience

Prices $335.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (Biotin) - LS-C181388 from LifeSpan Bioscience

Prices $365.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (DY650) - LS-C181389 from LifeSpan Bioscience

Prices $365.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (DY488) - LS-C181390 from LifeSpan Bioscience

Prices $365.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (HRP) - LS-C181391 from LifeSpan Bioscience

Prices $365.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (DY550) - LS-C181392 from LifeSpan Bioscience

Prices $365.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AMBRA1 Antibody (aa1-50) IHC-plus™ - LS-B7940 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-AMBRA1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7940 dilution 10 ug/ml.

Anti-AMBRA1 Antibody - LS-C192358 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of AMBRA1 pAb in extracts from MCF7 and HepG2 cells.

Anti-AMBRA1 Antibody - LS-C331248 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of MCF7 cell and HepG2 cell lysate using AMBRA1 antibody.

Anti-AMBRA1 Antibody (aa1200-1250) - LS-C289298 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human AMBRA1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-AMBRA1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Anti-AMBRA1 Antibody (aa27-55) - LS-C156304 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with AMBRA1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-AMBRA1 Antibody (N-Terminus) - LS-C53123 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 50 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of Ambra1 in Human Brain cells with Ambra1 antibody at 20 ug/ml.
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