DFFB

Gene Symbol DFFB
Entrez Gene 1677
Alt Symbol CAD, CPAN, DFF-40, DFF2, DFF40
Species Human
Gene Type protein-coding
Description DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Other Description DNA fragmentation factor 40 kDa subunit|DNA fragmentation factor subunit beta|caspase-activated DNase|caspase-activated deoxyribonuclease|caspase-activated nuclease
Swissprots Q9BYI4 O76075 Q5SR22 Q9BYI6 O60521 Q9BYI5
Accessions EAW71485 EAW71486 EAW71487 EAW71488 EAW71489 EAW71490 EAW71491 O76075 AB013918 BAA32250 AB028911 BAB40447 AB028912 BAB40448 AB028913 BAB40449 AF039210 AAC39709 AF064019 AAC39920 AF409060 AAL02005 AF409061 AAL02006 AF409062 AAL02007 AK027141 AK290877 BAF83566 AK303065 BAG64182 AK309316 AY438267 AAR05821 AY438268 AAR05822 BC032827 AAH32827 BC048797 AAH48797 DC368011 XM_011540863 XP_011539165 XM_011540864 XP_011539166 XM_011540865 XP_011539167 XR_946563 XR_946564 XR_946565 NM_001282669 NP_001269598 NM_004402 NP_004393 NR_104222 NM_001004285 NM_001004286
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.
Subcellular Location Cytoplasm. Nucleus.
Top Pathways Apoptosis

CAD (G-11) - sc-393029 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAD (G-11): sc-393029. Western blot analysis of CAD expression in LNCaP whole cell lysate.

CAD (C-19) - sc-8259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CAD (C-19): sc-8259. Western blot analysis of CAD expression in K-562 nuclear extract.

CAD (F-11) - sc-374067 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAD (F-11): sc-374067. Western blot analysis of CAD expression in K-562 (A) and Jurkat (B) nuclear extracts.

CAD (FL-338) - sc-8342 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CAD (FL-338): sc-8342. Western blot analysis of CAD expression in RAW 309 Cr.1 whole cell lysate.

CAD (K-13) - sc-34156 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CAD (H-1) - sc-5295 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CAD (H-1): sc-5295. Western blot analysis of CAD expression in Jurkat nuclear extract (A) and LNCaP whole cell lysate (B).

CAD (N-19) - sc-8258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CAD (N-19): sc-8258. Western blot analysis of CAD expression in K-562 (A) and phorbol-treated Jurkat (B) nuclear extracts.

Anti-DFFB antibody - ab8406 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC image of DDFB staining in human ovary formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8406, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-DFFB antibody - ab8407 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-DFFB antibody (ab8407) at 0.5 µg/mlLane 2 : Anti-DFFB antibody (ab8407) at 1 µg/mlLane 3 : Anti-DFFB antibody (ab8407) at 2 µg/mlLane 1 : Jurkat cell lysate Lane 2 : Jurkat cell lysateLane 3 : Jurkat cell lysateObserved band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-DFFB antibody - ab69438 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DFFB antibody (ab69438) at 1 µg/mlLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 5 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DFFB antibody - ab103435 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DFFB antibody (ab103435) at 1/100 dilution + 293 cells

Anti-DFFB antibody - ab8408 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DFFB antibody (ab8408) at 1/500 dilutionLane 1 : Jurkat whole cell lysateLane 2 : K562 whole cell lysateObserved band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-DFFB antibody [2263C6a] - ab50744 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2263C6a
Anti-DFFB antibody [2263C6a] (ab50744) + immunized recombinant protein

Anti-DFFB antibody [36A349] - ab13592 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 36A349
Anti-DFFB antibody [36A349] (ab13592) at 2 µg/ml + 30ug HeLa cell lysate

Anti-DFFB antibody [4B8] - ab71083 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4B8
All lanes : Anti-DFFB antibody [4B8] (ab71083) at 1/1000 dilutionLane 1 : A431 cell lysateLane 2 : Jurkat T cell lysateLane 3 : HeLa cell lysateLane 4 : HepG2 cell lysate

Anti-DFFB antibody - N-terminal - ab62150 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DFFB antibody - N-terminal (ab62150) at 1/500 dilutionLane 1 : Jurkat whole cell lysateLane 2 : K562 whole cell lysate

Anti-DFFB Antibody (aa205-222) - LS-C14842 from LifeSpan Bioscience

Prices $345.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-DFFB Antibody (aa205-222) - LS-C14845 from LifeSpan Bioscience

Prices $590.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DFFB Antibody (aa205-222, clone 36A349) - LS-C534 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 36A349
Western blot of 30 ug of HeLa cell lysate with antibody at a dilution of 2 ug/ml.

Anti-DFFB Antibody - LS-C48159 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DFFB Antibody - LS-C64703 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-DFFB Antibody (aa314-329) - LS-C283 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 ug/ml.

Anti-DFFB Antibody (aa205-222) - LS-C83372 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DFFB Antibody (clone 2263C6a) - LS-C184398 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2263C6a

Anti-DFFB Antibody (N-Terminus) - LS-C188967 from LifeSpan Bioscience

Prices $425.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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