Gene Symbol | RNF168 |
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Entrez Gene | 165918 |
Alt Symbol | hRNF168 |
Species | Human |
Gene Type | protein-coding |
Description | ring finger protein 168, E3 ubiquitin protein ligase |
Other Description | E3 ubiquitin-protein ligase RNF168 |
Swissprots | Q96NS4 Q8IYW5 Q8NA67 |
Accessions | EAW53649 EAW53650 EAW53651 Q8IYW5 AK054732 BAB70801 AK093113 BAC04060 AK097068 BC017442 BC029433 BC033791 AAH33791 BC047483 XM_011512489 XP_011510791 NM_152617 NP_689830 |
Function | E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and H2AX and amplifies the RNF8- dependent H2A ubiquitination, promoting the formation of 'Lys-63'- linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1. Also recruited at DNA interstrand cross-links (ICLs) sites and promotes accumulation of 'Lys-63'-linked ubiquitination of histones H2A and H2AX, leading to recruitment of FAAP20/C1orf86 and Fanconi anemia (FA) complex, followed by interstrand cross-link repair. H2A ubiquitination also mediates the ATM-dependent transcriptional silencing at regions flanking DSBs in cis, a mechanism to avoid collision between transcription and repair intermediates. Al |
Subcellular Location | Nucleus {ECO:0000269|PubMed:19203578, ECO:0000269|PubMed:19203579, ECO:0000269|PubMed:19500350, ECO:0000269|PubMed:21041483, ECO:0000269|PubMed:22742833}. Note=Localizes to double-strand breaks (DSBs) sites of DNA damage. |
RNF168 CRISPR/Cas9 KO Plasmid (h) - sc-402174 from Santa Cruz Biotechnology
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RNF168 HDR Plasmid (h) - sc-402174-HDR from Santa Cruz Biotechnology
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RNF168 Double Nickase Plasmid (h) - sc-402174-NIC from Santa Cruz Biotechnology
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RNF168 Double Nickase Plasmid (h2) - sc-402174-NIC-2 from Santa Cruz Biotechnology
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RNF168 CRISPR Activation Plasmid (h) - sc-402174-ACT from Santa Cruz Biotechnology
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RNF168 CRISPR Activation Plasmid (h2) - sc-402174-ACT-2 from Santa Cruz Biotechnology
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RNF168 Lentiviral Activation Particles (h) - sc-402174-LAC from Santa Cruz Biotechnology
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RNF168 Lentiviral Activation Particles (h2) - sc-402174-LAC-2 from Santa Cruz Biotechnology
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RNF168 siRNA (h) - sc-78089 from Santa Cruz Biotechnology
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RNF168 shRNA Plasmid (h) - sc-78089-SH from Santa Cruz Biotechnology
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RNF168 shRNA (h) Lentiviral Particles - sc-78089-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU011891 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN18400 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND18400 from Sigma-Aldrich
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RNF168 siRNA (Human) - CRJ6036 from Cohesion Biosciences
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RNF168 RNAi - H00165918-R01 from Novus Biologicals
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RNF168 - MBS8239320 from MyBioSource
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shRNA set against Human RNF168 (NM_152617.3) - SHH396928 from Creative biogene
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Human RNF168 siRNA - orb276537 from Biorbyt
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RNF168 shRNA Plasmid (Human) - CHJ6036 from Cohesion Biosciences
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