ATG14

Gene Symbol ATG14
Entrez Gene 22863
Alt Symbol ATG14L, BARKOR, KIAA0831
Species Human
Gene Type protein-coding
Description autophagy related 14
Other Description ATG14 autophagy related 14 homolog|Beclin 1-Interacting protein|autophagy-related protein 14-like protein|beclin 1-associated autophagy-related key regulator
Swissprots B7ZWP5 Q32MK8 O94920 A8K9U5 Q32MK7 A6NJE4 Q6ZNE5
Accessions CBX47442 EAW80672 EAW80673 EAW80674 Q6ZNE5 AA988343 AK131251 BAD18430 AK292810 BAF85499 AL049978 BC028015 BC109089 AAI09090 BC109090 AAI09091 BR000826 FAA00433 XM_011536563 XP_011534865 XM_011536564 XP_011534866 NM_014924 NP_055739
Function Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (By similarity). {ECO:0000250|UniProtKB:Q8CDJ3, ECO:0000269|PubMed:18843052, ECO:0000269|PubMed:19050071, ECO:0000269|PubMed:19270696, ECO:0000269|PubMed:20713597, ECO:0000269|PubMed:23878393}.
Subcellular Location Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Preautophagosomal structure membrane; Peripheral membrane protein. Note=Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes. Accumulates on highly curved PtdIns(3)P enriched autophagic membrane via its BATS domain to sense and maintain membrane curvature (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity). {ECO:0000250}.
Top Pathways Regulation of autophagy

SignalSilence ® Atg14 siRNA I - 6286 from Cell Signaling Technology

Category siRNA
Prices $256.00
Sizes 300 µl (3 nmol)
Species Reactivities Human
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Atg14 siRNA I (+) or SignalSilence ® Atg14 siRNA II #6287 (+), using Atg14 Antibody #5504 (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Atg14 Antibody confirms silencing of Atg14 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.

SignalSilence ® Atg14 siRNA II - 6287 from Cell Signaling Technology

Category siRNA
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® Atg14 siRNA I #6286 (+) or SignalSilence ® Atg14 siRNA II (+), using

Atg14 CRISPR/Cas9 KO Plasmid (h) - sc-402883 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 HDR Plasmid (h) - sc-402883-HDR from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 Double Nickase Plasmid (h) - sc-402883-NIC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 Double Nickase Plasmid (h2) - sc-402883-NIC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 CRISPR Activation Plasmid (h) - sc-402883-ACT from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 CRISPR Activation Plasmid (h2) - sc-402883-ACT-2 from Santa Cruz Biotechnology

Category CRISPR
Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 Lentiviral Activation Particles (h) - sc-402883-LAC from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 Lentiviral Activation Particles (h2) - sc-402883-LAC-2 from Santa Cruz Biotechnology

Prices $345.00
Sizes 20 µg
Species Reactivities Human

Atg14 siRNA (h) - sc-92229 from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Atg14 shRNA Plasmid (h) - sc-92229-SH from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

Atg14 shRNA (h) Lentiviral Particles - sc-92229-V from Santa Cruz Biotechnology

Category siRNA
Prices $258.00
Sizes 10 µm
Species Reactivities Human

MISSION® esiRNA - EHU132031 from Sigma-Aldrich

Category siRNA

CompoZr® Knockout ZFN Kit - CKOZFN3645 from Sigma-Aldrich

Category ZFN Kit
Prices $4,999.00
Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3645 from Sigma-Aldrich

Category ZFN Kit
Prices $3,999.00
Sizes 1 kt

MISSION® 3′UTR Lenti GoClone™ - HUTR13907 from Sigma-Aldrich

Category miRNA
Prices $350.00
Sizes hutr13907

ATG14 siRNA (Human) - CRH7821 from Cohesion Biosciences

Category siRNA
Prices $340.00, $510.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

ATG14 - MBS8220197 from MyBioSource

Prices $365.00, $515.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

shRNA set against Human ATG14 (NM_014924.4) - SHH242050 from Creative biogene

Category siRNA
Species Reactivities Human

Human ATG14 siRNA - orb268323 from Biorbyt

Category siRNA
Prices $485.00, $629.00
Sizes 15 nmol, 30 nmol
Species Reactivities Human

ATG14 shRNA Plasmid (Human) - CHH7821 from Cohesion Biosciences

Category siRNA
Prices $500.00, $750.00
Sizes 150 µg, 300 µg
Species Reactivities Human

ATG14 miRNA 3'UTR clone - MiUTR4H-TG00592 from Creative biogene

Species Reactivities Human
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