RFWD2

Gene Symbol RFWD2
Entrez Gene 64326
Alt Symbol COP1, RNF200
Species Human
Gene Type protein-coding
Description ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
Other Description E3 ubiquitin-protein ligase RFWD2|RING finger protein 200|constitutive photomorphogenesis protein 1 homolog|putative ubiquitin ligase COP1
Swissprots Q6H103 E9PKI0 X5D9B4 Q504W6 Q9H6L7 Q8NHY2
Accessions DAA01050 EAW90999 EAW91000 EAW91001 EAW91002 Q8NHY2 AF508940 AAM34692 AF527539 AAQ08989 AK001278 BAG50884 AK314750 BAG37289 AY509921 AAS82851 BC020845 AAH20845 BC039723 BC094728 AAH94728 BG717002 BX537932 CA450176 CD101686 KJ534928 AHW56568 KJ534929 AHW56569 KJ534930 AHW56570 KJ534931 AHW56571 KJ534932 AHW56572 KJ534933 AHW56573 KJ534992 AHW56632 KJ535061 AHW56700 KJ535076 AHW56715 XM_005245447 XP_005245504 XM_005245448 XP_005245505 XM_006711487 XP_006711550 XM_011509881 XP_011508183 XM_011509882 XP_011508184 XM_011509883 XP_011508185 XM_011509884 XP_011508186 XM_011509885 XP_011508187 XM_011509886 XP_011508188 XM_011509887 XP_011508189 XM_011509888 XP_011508190 XM_011509889 XP_011508191 XM_011509890 XP_011508192 NM_001001740 NP_001001740 NM_001286644 NP_001273573 NM_022457 NP_071902
Function E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal deg
Subcellular Location Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles.
Tissue Specificity Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. {ECO:0000269|PubMed:12615916, ECO:0000269|PubMed:15103385}.
Top Pathways p53 signaling pathway, Ubiquitin mediated proteolysis

COP1 (B-12) - sc-166799 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
COP1 (B-12): sc-166799. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.

COP1 (H-300) - sc-66837 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
COP1 (H-300): sc-66837. Western blot analysis of COP1 expression in JAR whole cell lysate.

COP1 (K-16) - sc-34592 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
COP1 (K-16): sc-34592. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.

COP1 (S-18) - sc-34594 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
COP1 (S-18): sc-34594. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic staining of decidual cells.

COP1 (N-20) - sc-34593 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-COP1 (phospho S387) antibody - ab92579 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-COP1 (phospho S387) antibody (ab92579) at 1/500 dilutionLane 1 : K562 cells treated with UV (15 min) Lane 2 : K562 cells treated with UV (15mins) with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-COP1 antibody - ab56400 from Abcam

Prices $392.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-COP1 antibody - ab70889 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-COP1 antibody (ab70889) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-COP1 antibody - ab70890 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). Antibodies: Lane 1: ab70889 at 3µg/ml for IP. Lane 2: ab70890 at 3µg/ml for IP. Lane 3: ab70891 at 3µg/ml for IP. Lane 4: IgG control Subsequent Western Blotting was carried out using ab70889 at 1µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-COP1 antibody - ab116122 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-COP1 antibody (ab116122) at 1 µg/ml + Mouse thymus lysate at 10 µg

Anti-COP1 antibody - ab138130 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-COP1 antibody (ab138130) at 1/500 dilution + HepG2 cell lysate at 30 µg

Anti-COP1 antibody - N-terminal - ab189790 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-COP1 antibody - N-terminal (ab189790) at 1/1000 dilutionLane 1 : total cell extracts from HeLaLane 2 : total cell extracts from Jurkat

Anti-RFWD2 / COP1 Antibody (aa672-701, AP) - LS-C223872 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa672-701, APC) - LS-C223873 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa672-701, Biotin) - LS-C223874 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa672-701, FITC) - LS-C223875 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa672-701, PE) - LS-C223877 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa672-701, Azide-free, HRP) - LS-C223876 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa650-699) - LS-C200590 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HepG2 cells using RFWD2 antibody.

Anti-RFWD2 / COP1 Antibody (aa1-50) - LS-C287734 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa100-150) - LS-C287735 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa353-402) - LS-C291251 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa672-701) - LS-C324630 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (aa300-350) - LS-C287742 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RFWD2 / COP1 Antibody (phospho-Ser387) - LS-C199537 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from K562 cells treated with UV 15', using RFWD2 (Phospho-Ser387) Antibody. The lane on the right was incubated with synthetic peptide.
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