RFWD2

Gene Symbol	RFWD2
Entrez Gene	64326
Alt Symbol	COP1, RNF200
Species	Human
Gene Type	protein-coding
Description	ring finger and WD repeat domain 2, E3 ubiquitin protein ligase
Other Description	E3 ubiquitin-protein ligase RFWD2|RING finger protein 200|constitutive photomorphogenesis protein 1 homolog|putative ubiquitin ligase COP1
Swissprots	Q6H103 E9PKI0 X5D9B4 Q504W6 Q9H6L7 Q8NHY2
Accessions	DAA01050 EAW90999 EAW91000 EAW91001 EAW91002 Q8NHY2 AF508940 AAM34692 AF527539 AAQ08989 AK001278 BAG50884 AK314750 BAG37289 AY509921 AAS82851 BC020845 AAH20845 BC039723 BC094728 AAH94728 BG717002 BX537932 CA450176 CD101686 KJ534928 AHW56568 KJ534929 AHW56569 KJ534930 AHW56570 KJ534931 AHW56571 KJ534932 AHW56572 KJ534933 AHW56573 KJ534992 AHW56632 KJ535061 AHW56700 KJ535076 AHW56715 XM_005245447 XP_005245504 XM_005245448 XP_005245505 XM_006711487 XP_006711550 XM_011509881 XP_011508183 XM_011509882 XP_011508184 XM_011509883 XP_011508185 XM_011509884 XP_011508186 XM_011509885 XP_011508187 XM_011509886 XP_011508188 XM_011509887 XP_011508189 XM_011509888 XP_011508190 XM_011509889 XP_011508191 XM_011509890 XP_011508192 NM_001001740 NP_001001740 NM_001286644 NP_001273573 NM_022457 NP_071902
Function	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal deg
Subcellular Location	Nucleus speckle. Cytoplasm. Note=In the nucleus, it forms nuclear speckles.
Tissue Specificity	Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. {ECO:0000269|PubMed:12615916, ECO:0000269|PubMed:15103385}.
Top Pathways	p53 signaling pathway, Ubiquitin mediated proteolysis

• Antibodies
• Assays/Kits
• Proteins/Peptides
• siRNAs/Functional Genomics
• cDNA Clones
• Cell/Tissue/Blood Products

COP1 CRISPR/Cas9 KO Plasmid (h) - sc-402776 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 HDR Plasmid (h) - sc-402776-HDR from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 Double Nickase Plasmid (h) - sc-402776-NIC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 Double Nickase Plasmid (h2) - sc-402776-NIC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 CRISPR Activation Plasmid (h) - sc-402776-ACT from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 CRISPR Activation Plasmid (h2) - sc-402776-ACT-2 from Santa Cruz Biotechnology Category CRISPR Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 Lentiviral Activation Particles (h) - sc-402776-LAC from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 Lentiviral Activation Particles (h2) - sc-402776-LAC-2 from Santa Cruz Biotechnology Prices $345.00 Sizes 20 µg Species Reactivities Human

COP1 siRNA (h) - sc-45541 from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

COP1 shRNA Plasmid (h) - sc-45541-SH from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

COP1 shRNA (h) Lentiviral Particles - sc-45541-V from Santa Cruz Biotechnology Category siRNA Prices $258.00 Sizes 10 µm Species Reactivities Human

COP1 RNAi - H00064326-R01 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

COP1 RNAi - H00064326-R02 from Novus Biologicals Category siRNA Prices $399.00 Sizes 20 nmol Species Reactivities Human

MISSION® esiRNA - EHU061271 from Sigma-Aldrich Category siRNA Prices $140.00, $250.00 Sizes 20 µg, 50 µg

CompoZr® Knockout ZFN Kit - CKOZFN1299 from Sigma-Aldrich Category ZFN Kit Prices $4,999.00 Sizes 1 kt

CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1299 from Sigma-Aldrich Category ZFN Kit Prices $3,999.00 Sizes 1 kt

MISSION® 3′UTR Lenti GoClone™ - HUTR02231 from Sigma-Aldrich Category miRNA Prices $350.00 Sizes hutr02231

RFWD2 siRNA (Human) - CRJ1985 from Cohesion Biosciences Category siRNA Prices $340.00, $510.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

RFWD2 - MBS8234531 from MyBioSource Prices $365.00, $515.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

shRNA set against Human RFWD2 (NM_022457.5) - SHH392364 from Creative biogene Category siRNA Species Reactivities Human

shRNA set against Human RFWD2(NM_022457.5) - SHR177680 from Creative biogene Category siRNA Species Reactivities Human

shRNA set against Human RFWD2(NM_001001740.2) - SHR177698 from Creative biogene Category siRNA Species Reactivities Human

Human RFWD2 siRNA - orb272486 from Biorbyt Category siRNA Prices $485.00, $629.00 Sizes 15 nmol, 30 nmol Species Reactivities Human

RFWD2 shRNA Plasmid (Human) - CHJ1985 from Cohesion Biosciences Category siRNA Prices $500.00, $750.00 Sizes 150 µg, 300 µg Species Reactivities Human

RFWD2 miRNA 3'UTR clone - MiUTR1H-08760 from Creative biogene Species Reactivities Human

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