PURA

Gene Symbol PURA
Entrez Gene 5813
Alt Symbol MRD31, PUR-ALPHA, PUR1, PURALPHA
Species Human
Gene Type protein-coding
Description purine-rich element binding protein A
Other Description purine-rich single-stranded DNA-binding protein alpha|transcriptional activator protein Pur-alpha
Swissprots Q00577
Accessions EAW62076 Q00577 BC036087 AAH36087 BC110574 AAI10575 BC110594 AAI10595 BT019388 AAV38195 CK819077 M96684 AAA60229 X91648 NM_005859 NP_005850
Function This is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene. May play a role in the initiation of DNA replication and in recombination.
Subcellular Location Nucleus.

Purα (H-48) - sc-382252 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Purα (S-14) - sc-165317 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Purα (T-13) - sc-165318 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Purα (80-L) - sc-130397 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Purα (80-L): sc-130397. Western blot analysis of Pura expression in human skeletal muscle tissue extract.

Anti-PURA antibody - ab79936 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PURA antibody (ab79936) at 1 µg/ml + Brain (Human) Tissue Lysate - adult normal tissue (ab29466) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PURA antibody - ab77734 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-PURA antibody (ab77734) at 1 µg/ml + Recombinant tagged human PURA fragment at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-PURA antibody - ab84928 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PURA antibody (ab84928) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PURA antibody - ab86534 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PURA antibody (ab86534) at 1 µg/ml + HepG2 Cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-PURA antibody - ab116267 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PURA antibody (ab116267) at 1 µg/ml + Mouse thymus lysate at 10 µg

Anti-PURA antibody - ab125200 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of PURA by Western Blot of Immunprecipitate. Lane 1; an antibody to PURA at 1µg/ml staining PURA in HeLa whole cell lysate immunoprecipitated using ab125200 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). The lysate in lane 2 is precipitated with an IgG control antibody. Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-PURALPHA / PURA Antibody - LS-C126189 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PURALPHA / PURA Antibody (aa100-150) - LS-C290209 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PURALPHA / PURA Antibody (aa1-50) - LS-C290211 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PURALPHA / PURA Antibody (N-Terminus) - LS-C109753 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PURA antibody LS-C109753 Western blot of Fetal Stomach lysate.

Anti-PURALPHA / PURA Antibody (aa249-298) - LS-C145322 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PURA antibody LS-C145322 Western blot of Mouse Thymus lysate. Antibody concentration 1 ug/ml.

Anti-PURALPHA / PURA Antibody (clone 3F10) - LS-C139163 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3F10
PURA monoclonal antibody (M01), clone 3F10. Western blot of PURA expression in human skeletal muscle.

Anti-PURALPHA / PURA Antibody (clone 1C10) - LS-C139317 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C10
PURA monoclonal antibody (M02), clone 1C10. Western blot of PURA expression in PC-12.

Anti-PURALPHA / PURA Antibody (clone 3A9) - LS-C139173 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3A9
PURA monoclonal antibody (M05), clone 3A9. Western blot of PURA expression in PC-12.

Anti-PURALPHA / PURA Antibody (aa266-295) - LS-C162324 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
PURA Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PURALPHA / PURA Antibody (aa35-84) - LS-C101705 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western analysis of HepG2 cell lysate.

Anti-PURALPHA / PURA Antibody (aa108-157) IHC-plus™ - LS-B6784 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PURA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6784 concentration 5 ug/ml.

PURA Antibody - NBP2-20012 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PURA Antibody (1C10) - H00005813-M02 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C10

PURA Antibody - NBP1-92651 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: PURA Antibody [NBP1-92651] -   Samples:  Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells.   Antibodies: Affinity purified rabbit anti-Pur-alpha antibody NBP1-92651 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate.  Pur-alpha was more efficiently immunoprecipitated by rabbit anti-Pur-alpha antibody NBP1-92652, which recognizes a downstream epitope.   Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

PURA Antibody - H00005813-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: PURA Antibody [H00005813-A01] - Analysis of PURA expression in human fetal heart.
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