UPF1

Gene Symbol UPF1
Entrez Gene 5976
Alt Symbol HUPF1, NORF1, RENT1, pNORF1, smg-2
Species Human
Gene Type protein-coding
Description UPF1 regulator of nonsense transcripts homolog (yeast)
Other Description ATP-dependent helicase RENT1|delta helicase|nonsense mRNA reducing factor 1|regulator of nonsense transcripts 1|smg-2 homolog, nonsense mediated mRNA decay factor|up-frameshift mutation 1 homolog|up-frameshift suppressor 1 homolog|yeast Upf1p homolog
Swissprots Q86Z25 Q92900 O00239 Q92842 O43343
Accessions AAB94785 AAC09016 AIA19311 AIA19312 AIA19313 AIA19314 AIA19315 AIA19316 AIA19317 AIA19318 AIA19319 AIA19320 AIA19321 AIA19322 AIA19323 AIA19324 AIA19325 AIA19326 AIA19327 AIA19328 EAW84738 EAW84739 EAW84740 EAW84741 EAW84742 Q92900 AF074016 AAC26788 AK125797 BAC86297 AK128727 BAG54717 BC034291 BC039817 AAH39817 BE243084 BM969672 BQ950083 BX331580 BX364728 CB960545 D86988 BAA19664 U59323 AAC51140 U65533 AAC50771 NM_001297549 NP_001284478 NM_002911 NP_002902
Function RNA-dependent helicase and ATPase required for nonsense- mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1- eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosph
Subcellular Location Cytoplasm. Cytoplasm, P-body. Nucleus. Note=Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.
Tissue Specificity Ubiquitous.
Top Pathways RNA transport, mRNA surveillance pathway

Upf1 (D15G6) Rabbit mAb - 12040 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D15G6
Western blot analysis of extracts from various cell lines using Upf1 (D15G6) Rabbit mAb.

Upf1 Antibody - 9435 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Upf1 Antibody.

Rent1 (B-4) - sc-390096 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent1 (B-4): sc-390096. Western blot analysis of Rent1 expression in non-transfected: sc-117752 (A) and human Rent1 transfected: sc-115858 (B) 293T whole cell lysates.

Rent1 (C-6) - sc-393594 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent1 (C-6): sc-393594. Western blot analysis of Rent1 expression in non-transfected 293T: sc-117752 (A), human Rent1 transfected 293T: sc-115858 (B), Sol8 (C), SH-SY5Y (D) and HeLa (E) whole cell lysates.

Rent1 (E-8) - sc-166091 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent1 (E-8): sc-166091. Western blot analysis of Rent1 expression in Sol8 whole cell lysate.

Rent1 (G-17) - sc-18258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent1 (G-17): sc-18258. Western blot analysis of Rent1 expression in non-transfected: sc-117752 (A) and human Rent1 transfected: sc-115858 (B) 293T whole cell lysates.

Rent1 (G-3) - sc-166092 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rent1 (G-3): sc-166092. Western blot analysis of Rent1 expression in Sol8 whole cell lysate.

Rent1 (H-300) - sc-48802 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rent1 (H-300): sc-48802. Western blot analysis of Rent1 expression in Sol8 (A), SK-N-SH (B) and L6 (C) whole cell lysates.

Rent1 (I-19) - sc-18259 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent1 (I-19): sc-18259. Western blot analysis of Rent1 expression in non-transfected: sc-117752 (A) and human Rent1 transfected: sc-115858 (B) 293T whole cell lysates.

Rent1 (P-14) - sc-18260 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rent1 (P-14): sc-18260. Western blot analysis of Rent1 expression in Sol8 whole cell lysate.

Anti-RENT1/hUPF1 antibody - ab196572 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of HeLa cells labeling RENT1/hUPF1 with ab196572 at a 1/50 dilution. Blue: DAPI for nuclear staining.

Anti-RENT1/hUPF1 antibody - ab92655 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RENT1/hUPF1 antibody (ab92655) at 1/500 dilutionLane 1 : Extract from NIH/3T3 cells (without competing peptide)Lane 2 : Extract from K562 cells (without competing peptide)Lane 3 : Extract from NIH/3T3 cells (with competing peptide)Lysates/proteins at 5 µg per lane.

Anti-RENT1/hUPF1 antibody - ab10510 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-RENT1/hUPF1 antibody - ab10534 from Abcam

Prices $378.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-RENT1/hUPF1 antibody - ab86057 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RENT1/hUPF1 antibody (ab86057) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : NIH3T3 whole cell lysate at 50 µg

Anti-RENT1/hUPF1 antibody - ab83095 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RENT1/hUPF1 antibody (ab83095) at 1 µg/ml + transfected 293T cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-RENT1/hUPF1 antibody - ab43408 from Abcam

Prices $359.00
Sizes 100 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-RENT1/hUPF1 antibody (ab43408) at 1/1000 dilutionLane 1 : a total protein extract from E coli with 50ng to 100 ng of a Tagged fusion protein of an irrelevant antigen Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (Tagged fusion protein)Lysates/proteins at 20 µg per lane.SecondaryRabbit anti-mouse IgG + IgM, (H+L) HRP conjugated, at 1/5000 dilution

Anti-RENT1/hUPF1 antibody [EP4682] - ab133564 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP4682
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling RENT1/hUPF1 with ab133564 at 1/100 dilution.

Anti-RENT1/hUPF1 antibody [EPR4681] - ab109363 from Abcam

Prices $397.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4681
All lanes : Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363) at 1/10000 dilutionLane 1 : HuT-78 cell lysateLane 2 : Raji cell lysateLane 3 : SH-SY5Y cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.Performed under reducing conditions.

Anti-RENT1/hUPF1 antibody - N-terminal - ab154903 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RENT1/hUPF1 antibody - N-terminal (ab154903) at 1/500 dilution + A431 whole cell lysate at 30 µg

Anti-RENT1 / UPF1 Antibody - LS-C90888 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-RENT1 / UPF1 Antibody (N-Terminus) - LS-C97613 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RENT1 / UPF1 Antibody - LS-C90889 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-RENT1 / UPF1 Antibody (C-Terminus) - LS-C97614 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RENT1 / UPF1 Antibody (C-Terminus) - LS-C90890 from LifeSpan Bioscience

Prices $575.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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