ARID5A

Gene Symbol ARID5A
Entrez Gene 10865
Alt Symbol MRF-1, MRF1, RP11-363D14
Species Human
Gene Type protein-coding
Description AT rich interactive domain 5A (MRF1-like)
Other Description ARID domain-containing protein 5A|AT-rich interactive domain-containing protein 5A|RFVG5814|modulator recognition factor 1|modulator recognition factor I
Swissprots Q6NX37 Q03989
Accessions EAW71359 EAW71360 EAW71361 EAW71362 EAW71363 EAW71364 EAW71365 EAW71366 Q03989 AK300173 BAG61953 AK304566 BAH14217 AK307768 AY358167 AAQ88534 BC032488 BC047390 BC067301 BC167773 AAI67773 BQ073595 BU734273 BX648918 M62324 AAA36325 XM_005263856 XP_005263913 XM_005263860 XP_005263917 XM_011510499 XP_011508801 XM_011510500 XP_011508802 XM_011510501 XP_011508803 NM_006673 NM_212481 NP_997646
Function Binds to AT-rich stretches in the modulator region upstream of the human cytomegalovirus major intermediate early gene enhancer. May act as repressor and down-regulate enhancer- dependent gene expressison. {ECO:0000269|PubMed:8649988}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00355, ECO:0000269|PubMed:8649988}.

Mrf-1 (C-14) - sc-137607 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-ARID5A antibody - ab81149 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab81149 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81149 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-ARID5A antibody - ab151449 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARID5A antibody (ab151449) at 1/5000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysate Lane 3 : HeLa whole cell lysateLysates/proteins at 30 µg per lane.

Anti-ARID5A antibody - ab81207 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ARID5A antibody (ab81207) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-ARID5A antibody - ab173596 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-ARID5A antibody (ab173596) at 1/100 dilution + A2058 cell lysate at 35 µg

Anti-ARID5A Antibody (aa382-411, AP) - LS-C208035 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (aa382-411, APC) - LS-C208036 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (aa382-411, Biotin) - LS-C208037 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (aa110-159) - LS-C81609 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-ARID5A Antibody (aa382-411, FITC) - LS-C208038 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (aa382-411, Azide-free, HRP) - LS-C208039 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (aa382-411, PE) - LS-C208040 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (N-Terminus) - LS-C111316 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ARID5A antibody LS-C111316 Western blot of COLO205 cell lysate.

Anti-ARID5A Antibody - LS-C306344 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-ARID5A Antibody (aa381-411) - LS-C319589 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARID5A Antibody (aa382-411) - LS-C159114 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ARID5A Antibody western blot of A2058 cell line lysates (35 ug/lane). The ARID5A antibody detected the ARID5A protein (arrow).

Anti-ARID5A Antibody (C-Terminus) IHC-plus™ - LS-B3563 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-ARID5A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3563 concentration 5 ug/ml.

ARID5A Antibody - NBP1-79441 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARID5A Antibody [NBP1-79441] - Sample Tissue: Human 721_B Antibody Dilution: 1.0 ug/ml

ARID5A Antibody - NBP1-79440 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARID5A Antibody [NBP1-79440] - Sample Tissue: 721_B, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane/lane, Gel Concentration: 0.12

ARID5A Antibody - NBP1-81037 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARID5A Antibody [NBP1-81037] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4

ARID5A Antibody - NBP2-15459 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARID5A Antibody [NBP2-15459] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:5000.

ARID5A Antibody - NBP1-79452 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARID5A Antibody [NBP1-79452] - COLO205 cells lysate, concentration 0.2-1 ug/ml.

ARID5A Antibody - H00010865-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: ARID5A Antibody [H00010865-B01P] - Analysis of ARID5A expression in transfected 293T cell line by ARID5A polyclonal antibody. Lane 1: ARID5A transfected lysate(47.3 KDa). Lane 2: Non-transfected lysate.

Anti-ARID5A (ab2) antibody produced in rabbit - SAB2100148 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ARID5A (ab2): <B>Cat. No. SAB2100148</B>: Immunohistochemistry of ARID5A in Human Prostate tissue with ARID5A antibody at 5.0 μg/mL.

Anti-ARID5A, (N-terminal) antibody produced in rabbit - SAB2103454 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ARID5A, antibody produced in rabbit: <B>Cat. No. SAB2103454</B>: Immunoblotting analysis of ARID5A in COLO205 cell lysate with ARID5A anitbody at 1 μg/mL.
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