SPEN

Gene Symbol SPEN
Entrez Gene 23013
Alt Symbol HIAA0929, MINT, RBM15C, SHARP
Species Human
Gene Type protein-coding
Description spen family transcriptional repressor
Other Description Msx2 interacting nuclear target (MINT) homolog|SMART/HDAC1 associated repressor protein|SMART/HDAC1-associated repressor protein|msx2-interacting protein|nuclear receptor transcription cofactor|spen homolog, transcriptional regulator
Swissprots Q9NWH5 Q9Y556 Q9H9A8 Q96T58 Q9UQ01
Accessions CBX47466 EAW51756 Q96T58 AB023146 BAA76773 AF085964 AF356524 AAK52750 AK000882 BAA91405 AK022949 BAB14324 AK092332 AK127577 AL096858 CAB51072 NM_015001 NP_055816
Function May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA. {ECO:0000250, ECO:0000269|PubMed:11331609, ECO:0000269|PubMed:12374742}.
Subcellular Location Nucleus {ECO:0000269|PubMed:11331609}. Note=Associates with chromatin.
Tissue Specificity Expressed at high level in brain, testis, spleen and thymus. Expressed at intermediate level in kidney, liver, mammary gland and skin.

SPEN (H-300) - sc-98675 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

SPEN (N-19) - sc-79097 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-SPEN antibody - ab72266 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation/ Western Blot of SPEN. Lane 1: ab72266 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72266 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 minutes.

Anti-SPEN / SHARP Antibody (aa1350-1400) - LS-C287946 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SPEN / SHARP Antibody (aa1503-1517) - LS-C55521 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SPEN / SHARP Antibody (aa3375-3425) - LS-C287948 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SPEN Antibody - NBP1-82952 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: SPEN Antibody [NBP1-82952] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

SPEN Antibody - NB100-58798 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SPEN Antibody [NB100-58798] - Detection of Human SHARP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHARP antibody NB100-58798 used for IP at 3 mcg/mg lysate. SHARP was also immunoprecipitated by rabbit anti-SHARP antibody Nb100-58799, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes.

SPEN Antibody - NB100-58799 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: SPEN Antibody [NB100-58799] - Detection of Human SHARP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SHARP antibody NB100-58799 used for IP at 3 mcg/mg lysate. SHARP was also immunoprecipitated by rabbit anti-SHARP antibody NB100-58798, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 3 minutes.

SPEN Antibody - NB100-2804 from Novus Biologicals

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SPEN antibody produced in rabbit - HPA015825 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-SPEN: <B>Cat. No. HPA015825</B>: Immunohistochemical staining of human rectum shows strong nuclear and slightly weaker cytoplasmic positivity in glandular cells.

SPEN--SHARP-Antibody-internal-region - AF2505a from Abgent, a WuXi AppTec company

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Goat Anti-SPEN / SHARP Antibody (OAEB00566) - OAEB00566 from Aviva Systems Biology

Prices $229.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody - AC21-0886 from Abcore

Prices $359.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (AMCA) - AC21-0886-01 from Abcore

Prices $495.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (AP) - AC21-0886-02 from Abcore

Prices $505.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (APC) - AC21-0886-03 from Abcore

Prices $515.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (APC-Cy5.5) - AC21-0886-04 from Abcore

Prices $539.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (APC-Cy7) - AC21-0886-05 from Abcore

Prices $539.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (Avidin) - AC21-0886-06 from Abcore

Prices $519.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (Biotin) - AC21-0886-07 from Abcore

Prices $519.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (BPE) - AC21-0886-08 from Abcore

Prices $499.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (Cy3) - AC21-0886-09 from Abcore

Prices $505.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (Cy5) - AC21-0886-10 from Abcore

Prices $505.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

Anti-SPEN Antibody (Cy5.5) - AC21-0886-11 from Abcore

Prices $505.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
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