| Gene Symbol | SPEN |
|---|---|
| Entrez Gene | 23013 |
| Alt Symbol | HIAA0929, MINT, RBM15C, SHARP |
| Species | Human |
| Gene Type | protein-coding |
| Description | spen family transcriptional repressor |
| Other Description | Msx2 interacting nuclear target (MINT) homolog|SMART/HDAC1 associated repressor protein|SMART/HDAC1-associated repressor protein|msx2-interacting protein|nuclear receptor transcription cofactor|spen homolog, transcriptional regulator |
| Swissprots | Q9NWH5 Q9Y556 Q9H9A8 Q96T58 Q9UQ01 |
| Accessions | CBX47466 EAW51756 Q96T58 AB023146 BAA76773 AF085964 AF356524 AAK52750 AK000882 BAA91405 AK022949 BAB14324 AK092332 AK127577 AL096858 CAB51072 NM_015001 NP_055816 |
| Function | May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2- mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA. {ECO:0000250, ECO:0000269|PubMed:11331609, ECO:0000269|PubMed:12374742}. |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:11331609}. Note=Associates with chromatin. |
| Tissue Specificity | Expressed at high level in brain, testis, spleen and thymus. Expressed at intermediate level in kidney, liver, mammary gland and skin. |
SPEN CRISPR/Cas9 KO Plasmid (h) - sc-406083 from Santa Cruz Biotechnology
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SPEN HDR Plasmid (h) - sc-406083-HDR from Santa Cruz Biotechnology
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SPEN Double Nickase Plasmid (h) - sc-406083-NIC from Santa Cruz Biotechnology
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SPEN Double Nickase Plasmid (h2) - sc-406083-NIC-2 from Santa Cruz Biotechnology
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SPEN CRISPR Activation Plasmid (h) - sc-406083-ACT from Santa Cruz Biotechnology
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SPEN CRISPR Activation Plasmid (h2) - sc-406083-ACT-2 from Santa Cruz Biotechnology
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SPEN Lentiviral Activation Particles (h) - sc-406083-LAC from Santa Cruz Biotechnology
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SPEN Lentiviral Activation Particles (h2) - sc-406083-LAC-2 from Santa Cruz Biotechnology
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SPEN siRNA (h) - sc-76556 from Santa Cruz Biotechnology
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SPEN shRNA Plasmid (h) - sc-76556-SH from Santa Cruz Biotechnology
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SPEN shRNA (h) Lentiviral Particles - sc-76556-V from Santa Cruz Biotechnology
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SPEN RNAi - H00023013-R01 from Novus Biologicals
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MISSION® esiRNA - EHU000931 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20458 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20458 from Sigma-Aldrich
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SPEN siRNA (Human) - CRH7929 from Cohesion Biosciences
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SPEN - MBS8207632 from MyBioSource
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shRNA set against Human SPEN (NM_015001.2) - SHH416517 from Creative biogene
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shRNA set against Human SPEN(NM_015001.2) - SHL027930 from Creative biogene
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Human SPEN siRNA - orb268431 from Biorbyt
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SPEN shRNA Plasmid (Human) - CHH7929 from Cohesion Biosciences
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SPEN miRNA 3'UTR clone - MiUTR1H-09830 from Creative biogene
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