| Gene Symbol | Aifm1 |
|---|---|
| Entrez Gene | 26926 |
| Alt Symbol | AIF, AIFsh2, Hq, Pdcd8 |
| Species | Mouse |
| Gene Type | protein-coding |
| Description | apoptosis-inducing factor, mitochondrion-associated 1 |
| Other Description | apoptosis-inducing factor 1, mitochondrial|harlequin|programmed cell death protein 8 |
| Swissprots | Q9Z0X1 |
| Accessions | EDL29077 Q9Z0X1 AA261021 AF100927 AAD16435 AK133764 AK165762 AK180729 AK211178 AK211196 AK219625 BC003292 AAH03292 BC024882 BY250260 DQ016499 AAY84740 NM_001290364 NP_001277293 NM_012019 NP_036149 |
| Function | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis,and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity). {ECO:0000250}. |
| Subcellular Location | Mitochondrion intermembrane space {ECO:0000269|PubMed:9989411}. Mitochondrion inner membrane {ECO:0000250}. Cytoplasm {ECO:0000250}. Cytoplasm, perinuclear region {ECO:0000250}. Nucleus {ECO:0000269|PubMed:9989411}. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region (By similarity). {ECO:0000250}. |
| Top Pathways | Apoptosis |
AIF CRISPR/Cas9 KO Plasmid (m) - sc-424143 from Santa Cruz Biotechnology
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AIF HDR Plasmid (m) - sc-424143-HDR from Santa Cruz Biotechnology
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AIF Double Nickase Plasmid (m) - sc-424143-NIC from Santa Cruz Biotechnology
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AIF Double Nickase Plasmid (m2) - sc-424143-NIC-2 from Santa Cruz Biotechnology
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AIF CRISPR Activation Plasmid (m) - sc-424143-ACT from Santa Cruz Biotechnology
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AIF CRISPR Activation Plasmid (m2) - sc-424143-ACT-2 from Santa Cruz Biotechnology
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AIF Lentiviral Activation Particles (m) - sc-424143-LAC from Santa Cruz Biotechnology
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AIF Lentiviral Activation Particles (m2) - sc-424143-LAC-2 from Santa Cruz Biotechnology
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AIF siRNA (m) - sc-29194 from Santa Cruz Biotechnology
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AIF shRNA Plasmid (m) - sc-29194-SH from Santa Cruz Biotechnology
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AIF shRNA (m) Lentiviral Particles - sc-29194-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EMU032921 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN26471 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND26471 from Sigma-Aldrich
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AIFM1 siRNA (Mouse) - CRM4815 from Cohesion Biosciences
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AIFM1 - MBS8209391 from MyBioSource
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shRNA set against Mouse AIFM1 (NM_012019.2) - SHH233922 from Creative biogene
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shRNA set against Mouse Aifm1(NM_012019.2) - SHG048403 from Creative biogene
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Mouse AIFM1 siRNA - orb284291 from Biorbyt
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AIFM1 shRNA Plasmid (Mouse) - CHM4815 from Cohesion Biosciences
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AIFM1 miRNA 3'UTR clone - MiUTR1M-01343 from Creative biogene
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