Gene Symbol | CHST14 |
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Entrez Gene | 113189 |
Alt Symbol | ATCS, D4ST1, EDSMC1, HNK1ST |
Species | Human |
Gene Type | protein-coding |
Description | carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14 |
Other Description | carbohydrate sulfotransferase 14|dermatan 4 sulfotransferase 1 |
Swissprots | Q8NCH0 Q6UXA0 Q96P94 Q6PJ31 |
Accessions | EAW92419 Q8NCH0 AB066595 BAB84097 AF282905 AAK69530 AF401222 AAK92532 AK074739 BAC11172 AY358446 AAQ88811 BC009883 BC023653 AAH23653 BC049214 AAH49214 BC053633 AAH53633 CR997569 HQ448147 ADQ32628 NM_130468 NP_569735 |
Function | Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. GlcUA to IdoUA. Appears to have an important role in the formation of the cerbellar neural network during postnatal brain development. {ECO:0000269|PubMed:19661164}. |
Subcellular Location | Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
Tissue Specificity | Widely expressed. Expressed at high level in pituitary gland, placenta, uterus and thyroid. {ECO:0000269|PubMed:11470797}. |
Top Pathways | Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate |
D4ST CRISPR/Cas9 KO Plasmid (h) - sc-414018 from Santa Cruz Biotechnology
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D4ST HDR Plasmid (h) - sc-414018-HDR from Santa Cruz Biotechnology
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D4ST Double Nickase Plasmid (h) - sc-414018-NIC from Santa Cruz Biotechnology
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D4ST Double Nickase Plasmid (h2) - sc-414018-NIC-2 from Santa Cruz Biotechnology
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D4ST CRISPR Activation Plasmid (h) - sc-414018-ACT from Santa Cruz Biotechnology
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D4ST CRISPR Activation Plasmid (h2) - sc-414018-ACT-2 from Santa Cruz Biotechnology
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D4ST Lentiviral Activation Particles (h) - sc-414018-LAC from Santa Cruz Biotechnology
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D4ST Lentiviral Activation Particles (h2) - sc-414018-LAC-2 from Santa Cruz Biotechnology
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D4ST siRNA (h) - sc-77318 from Santa Cruz Biotechnology
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D4ST shRNA Plasmid (h) - sc-77318-SH from Santa Cruz Biotechnology
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D4ST shRNA (h) Lentiviral Particles - sc-77318-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU033551 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN6108 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6108 from Sigma-Aldrich
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CHST14 siRNA (Human) - CRJ4315 from Cohesion Biosciences
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CHST14 - MBS8230434 from MyBioSource
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shRNA set against Human CHST14 (NM_130468.3) - SHH263765 from Creative biogene
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Human CHST14 siRNA - orb274816 from Biorbyt
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CHST14 shRNA Plasmid (Human) - CHJ4315 from Cohesion Biosciences
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CHST14 miRNA 3'UTR clone - MiUTR3H-09993 from Creative biogene
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