Gene Symbol | ALOX12B |
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Entrez Gene | 242 |
Alt Symbol | 12R-LOX, ARCI2 |
Species | Human |
Gene Type | protein-coding |
Description | arachidonate 12-lipoxygenase, 12R type |
Other Description | 12R-lipoxygenase|arachidonate 12-lipoxygenase, 12R-type|epidermis-type lipoxygenase 12 |
Swissprots | O75342 |
Accessions | CAC34520 EAW90095 O75342 AF038461 AAC39770 AF059250 AAC79680 BC041058 AAH41058 NM_001139 NP_001130 |
Function | Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO:0000269|PubMed:21558561, ECO:0000269|PubMed:22441738, ECO:0000269|PubMed:9618483}. |
Subcellular Location | Cytoplasm {ECO:0000255|PROSITE- ProRule:PRU00726}. |
Tissue Specificity | Expressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue. {ECO:0000269|PubMed:9618483}. |
Top Pathways | Arachidonic acid metabolism |
Epidermis-type 12-LO CRISPR/Cas9 KO Plasmid (h) - sc-410534 from Santa Cruz Biotechnology
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Epidermis-type 12-LO HDR Plasmid (h) - sc-410534-HDR from Santa Cruz Biotechnology
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Epidermis-type 12-LO Double Nickase Plasmid (h) - sc-410534-NIC from Santa Cruz Biotechnology
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Epidermis-type 12-LO Double Nickase Plasmid (h2) - sc-410534-NIC-2 from Santa Cruz Biotechnology
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Epidermis-type 12-LO CRISPR Activation Plasmid (h) - sc-410534-ACT from Santa Cruz Biotechnology
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Epidermis-type 12-LO CRISPR Activation Plasmid (h2) - sc-410534-ACT-2 from Santa Cruz Biotechnology
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Epidermis-type 12-LO Lentiviral Activation Particles (h) - sc-410534-LAC from Santa Cruz Biotechnology
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Epidermis-type 12-LO Lentiviral Activation Particles (h2) - sc-410534-LAC-2 from Santa Cruz Biotechnology
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Epidermis-type 12-LO siRNA (h) - sc-105333 from Santa Cruz Biotechnology
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Epidermis-type 12-LO shRNA Plasmid (h) - sc-105333-SH from Santa Cruz Biotechnology
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Epidermis-type 12-LO shRNA (h) Lentiviral Particles - sc-105333-V from Santa Cruz Biotechnology
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ALOX12B RNAi - H00000242-R01 from Novus Biologicals
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MISSION® esiRNA - EHU119511 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1924 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1924 from Sigma-Aldrich
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ALOX12B siRNA (Human) - CRH0169 from Cohesion Biosciences
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ALOX12B - MBS8201671 from MyBioSource
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shRNA set against Human ALOX12B (NM_001139.2) - SHH235346 from Creative biogene
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shRNA set against Human ALOX12B(NM_001139.2) - SHG031853 from Creative biogene
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Human ALOX12B siRNA - orb260671 from Biorbyt
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ALOX12B shRNA Plasmid (Human) - CHH0169 from Cohesion Biosciences
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ALOX12B miRNA 3'UTR clone - MiUTR1H-00312 from Creative biogene
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