DAB2

Gene Symbol DAB2
Entrez Gene 1601
Alt Symbol DOC-2, DOC2
Species Human
Gene Type protein-coding
Description Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila)
Other Description adaptor molecule disabled-2|differentially expressed in ovarian carcinoma 2|differentially-expressed protein 2|disabled homolog 2|disabled homolog 2, mitogen-responsive phosphoprotein
Swissprots Q9UK04 Q9BTY0 Q13598 P98082 A6NES5
Accessions AAF23161 EAW55989 EAW55990 EAW55991 EAW55992 EAW55993 EAW55994 P98082 AF188298 AAF05540 AI739507 AK024965 AK092836 AK130136 AK297984 BAG60293 AK314381 BAG37007 BC003064 AAH03064 BU742719 CB989218 DQ891872 ABM82798 EU176643 ABW03444 L16886 AAA93195 U39050 AAC50824 U53446 AAA98975 NM_001244871 NP_001231800 NM_001343 NP_001334
Function Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Involved in CFTR trafficking to the late endosome. Involved in several rec
Subcellular Location Cytoplasm. Cytoplasmic vesicle, clathrin- coated vesicle membrane. Membrane, clathrin-coated pit. Note=Colocalizes with large insert-containing isoforms of MYO6 at clathrin-coated pits/vesicles. During mitosis is progressively displaced from the membrane and translocated to the cytoplasm.
Tissue Specificity Expressed in deep invaginations, inclusion cysts and the surface epithelial cells of the ovary. Also expressed in breast epithelial cells, spleen, thymus, prostate, testis, macrophages, fibroblasts, lung epithelial cells, placenta, brain stem, heart and small intestine. Expressed in kidney proximal tubular epithelial cells (at protein level). {ECO:0000269|PubMed:10340382, ECO:0000269|PubMed:15134832, ECO:0000269|PubMed:9620555}.
Top Pathways Endocytosis

Dab2 Antibody - 8582 from Cell Signaling Technology

Prices $239.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A172 and A-204 cells using Dab2 Antibody.

Dab2 (D7O9T) Rabbit mAb - 12906 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7O9T
Western blot analysis of extracts from HeLa, A172, and OVCAR3 cells using Dab2 (D7O9T) Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457.

Dab2 (B-3) - sc-133077 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dab2 (B-3): sc-133077. Western blot analysis of Dab2 expression in HeLa (A) and H4 (B) whole cell lysates.

Dab2 (C-1) - sc-390942 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dab2 (C-1): sc-390942. Western blot analysis of Dab2 expression in HeLa whole cell lysate.

Dab2 (C-20) - sc-7832 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dab2 (C-20): sc-7832. Western blot analysis of Dab2 isoform expression in H4 (A), Y79 (B), 3T3-LI (C) and F9 (D) whole cell lysates and rat embryo tissue extract (E).

Dab2 (D-19) - sc-7828 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Dab2 (D-19): sc-7828. Western blot analysis of Dab2 isoform expression in H4 whole cell lysate.

Dab2 (D-6) - sc-136963 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dab2 (D-6): sc-136963. Western blot analysis of Dab2 expression in non-transfected: sc-117752 (A) and mouse Dab2 transfected: sc-119651 (B) 293T whole cell lysates.

Dab2 (H-110) - sc-13982 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Dab2 (H-110): sc-13982. Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Dab2 (E-11) - sc-136964 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Dab2 (E-11): sc-136964. Western blot analysis of Dab2 expression in non-transfected: sc-117752 (A) and mouse Dab2 transfected: sc-119651 (B) 293T whole cell lysates.

p-Dab2 (Ser 24) - sc-32573 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
p-Dab2 (Ser 24): sc-32573. Western blot analysis of Dab2 phosphorylation in non-transfected 293T: sc-117752 (A), mouse Dab2 transfected 293T: sc-119652 (B) and Y79 (C) whole cell lysates.

Anti-DAB2 antibody - ab33441 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DAB2 antibody (ab33441) at 2 µg/ml + Testis (Human) Tissue Lysate - adult normal tissue (ab30257) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-DAB2 antibody - ab166779 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling DAB2 with ab166779 at 3.75µg/ml.

Anti-DAB2 antibody - ab88590 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-DAB2 antibody (ab88590) at 1 µg/ml + human liver tissue lysate at 50 µgdeveloped using the ECL technique

Anti-DAB2 antibody - ab129288 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab129288, at 3.75&microg/ml, staining DAB2 in Formalin-fixed, Paraffin-embedded Human placenta tissue by Immunohistochemistry.

Anti-DAB2 antibody - ab137866 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DAB2 antibody (ab137866) at 1 µg/ml (Milk blocking 3%) + RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate at 25 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DAB2 antibody - ab20468 from Abcam

Prices $359.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-DAB2 antibody (ab20468) at 0.003 µg/ml + Human Kidney lysate at 30 µg

Anti-DAB2 antibody [EP2297Y] - ab76253 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2297Y
ab76253 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76253 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-DAB2 antibody [EPR2249] - ab133356 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2249
Anti-DAB2 antibody [EPR2249] (ab133356) at 1/1000 dilution + HeLa cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-DAB2 antibody - C-terminal - ab175950 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DAB2 antibody - C-terminal (ab175950) at 1/1000 dilution + A2058 cell line lysate at 35 µg

Anti-DAB2 Antibody (clone 1C8) - LS-C197016 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1C8

Anti-DAB2 Antibody (aa624-650, AP) - LS-C221981 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DAB2 Antibody (Internal, clone EPR2249) - LS-C105604 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2249
Western blot analysis on HeLa cell lysates using anti-Dab2 antibody (LS-C105604).

Anti-DAB2 Antibody (aa624-650, Biotin) - LS-C221983 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DAB2 Antibody (aa624-650, APC) - LS-C221982 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DAB2 Antibody (C-Terminus, clone EP2297Y) - LS-C105261 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2297Y
Western blot analysis on human pancreas tissue lysates using anti-Dab2 antibody (LS-C105261), 1:2000 dilution.
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