MTDH

Gene Symbol MTDH
Entrez Gene 92140
Alt Symbol 3D3, AEG-1, AEG1, LYRIC, LYRIC/3D3
Species Human
Gene Type protein-coding
Description metadherin
Other Description 3D3/LYRIC|astrocyte elevated gene 1|astrocyte elevated gene-1 protein|lysine-rich CEACAM1 co-isolated protein|metastasis adhesion protein|protein LYRIC
Swissprots Q8TCX3 Q6PK07 Q05DH2 Q86UE4 Q52QU9
Accessions EAW91760 EAW91761 EAW91762 Q86UE4 AF411226 AAO65771 AF501310 AAP30791 AK000745 AK123083 AK308030 AY082966 AAL92861 AY974040 AAX84832 BC001815 BC009324 AAH09324 BC014977 AAH14977 BC022825 BC023994 BC045642 AAH45642 JF432755 ADZ15972 XM_005251099 XP_005251156 XM_011517367 XP_011515669 XM_011517368 XP_011515670 XM_011517369 XP_011515671 XM_011517370 XP_011515672 NM_178812 NP_848927
Function Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF- kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. {ECO:0000269|PubMed:15927426, ECO:0000269|PubMed:16452207, ECO:0000269|PubMed:18316612, ECO:0000269|PubMed:19111877}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Cell junction, tight junction {ECO:0000250}. Nucleus, nucleolus {ECO:0000250}. Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. {ECO:0000250}.
Tissue Specificity Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. {ECO:0000269|PubMed:15927426}.

Lyric/Metadherin (D5Y8R) XP ® Rabbit mAb - 14065 from Cell Signaling Technology

Prices $109.00, $282.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5Y8R
Western blot analysis of extracts from HT-29 cells, transfected with lyric shRNA (+) or control shRNA (-), using Lyric/Metadherin (D5Y8R) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Lyric/Metadherin (2F11C3) Mouse mAb - 9596 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 2F11C3
Western blot analysis of extracts from various cell lines and tissue using Lyric/Metadherin (2F11C3) Mouse mAb.

Anti-LYRIC antibody - ab168190 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-LYRIC antibody (ab168190) at 1/500 dilutionLane 1 : LYRIC transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-LYRIC antibody - ab76742 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-LYRIC antibody (ab76742) at 1/500 dilutionLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg/ml per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-LYRIC antibody - ab113204 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of LYRIC by Western Blot of Immunprecipitate. An antibody to LYRIC at 1µg/ml staining LYRIC in HeLa whole cell lysate immunoprecipitated using ab113204at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-LYRIC antibody [EP4445] - ab124789 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP4445
All lanes : Anti-LYRIC antibody [EP4445] (ab124789) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : PC3 cell lysateLane 4 : PC12 cell lysateLane 5 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-MTDH / Metadherin Antibody (clone 2F11C3) IHC-plus™ - LS-B4118 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 2F11C3
Anti-MTDH antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4118 dilution 1:200.

Anti-MTDH / Metadherin Antibody - LS-C130727 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MTDH / Metadherin Antibody (N-Terminus) - LS-C153525 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MTDH / Metadherin Antibody (aa75-125) - LS-C289842 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MTDH / Metadherin Antibody (aa175-225) - LS-C289841 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MTDH / Metadherin Antibody (aa300-350) - LS-C289843 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MTDH / Metadherin Antibody (N-Terminus) IHC-plus™ - LS-B7530 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-MTDH antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7530 concentration 10 ug/ml.

Anti-MTDH / Metadherin Antibody (aa70-582, clone 1D3) - LS-C338840 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTDH.

Anti-MTDH / Metadherin Antibody (aa70-582, clone 1E7) - LS-C340638 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1E7
IHC of paraffin-embedded Human lymphoma tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MTDH / Metadherin Antibody (aa70-582, clone 1H1) - LS-C339149 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H1
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MTDH / Metadherin Antibody (aa70-582, clone 2A6) - LS-C339023 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A6
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTDH.

Anti-MTDH / Metadherin Antibody (aa70-582, clone 4C5) - LS-C338870 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4C5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTDH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTDH.

Anti-MTDH / Metadherin Antibody (aa70-582, clone 6H2) - LS-C339007 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6H2
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MTDH / Metadherin Antibody (aa70-582, clone 2H4) - LS-C338841 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2H4
IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MTDH / Metadherin Antibody (aa70-582, clone 2F11) - LS-C338842 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2F11
IHC of paraffin-embedded Human pancreas tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

Anti-MTDH / Metadherin Antibody (Internal, clone EP4445) IHC-plus™ - LS-B8026 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Monoclonal
Clone EP4445
Anti-3D3 / MTDH antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8026 dilution 1:50.

Anti-MTDH / Metadherin Antibody (aa70-582, clone 1B6) - LS-C339208 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B6
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MTDH mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

LYRIC Antibody (CL0401) - NBP2-30458 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone CL0401
Western Blot: LYRIC Antibody (CL0401) [NBP2-30458] - Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

LYRIC Antibody - AF7644 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Search more