FBL

Gene Symbol FBL
Entrez Gene 2091
Alt Symbol FIB, FLRN, RNU3IP1
Species Human
Gene Type protein-coding
Description fibrillarin
Other Description 34 kDa nucleolar scleroderma antigen|34-kD nucleolar scleroderma antigen|RNA, U3 small nucleolar interacting protein 1|histone-glutamine methyltransferase|rRNA 2'-O-methyltransferase fibrillarin
Swissprots Q6IAT5 B5BUE8 O75259 P22087 Q9UPI6
Accessions AAC28913 AAD15623 EAW56925 P22087 AB451384 BAG70198 AI085380 AK296950 BAG59496 AK308649 BC015218 AAH15218 BC019260 AAH19260 BC019609 AAH19609 BG831506 BT006830 AAP35476 BT020144 AAV38946 CR457069 CAG33350 DQ892314 ABM83240 DQ895517 ABM86443 M59849 AAA52453 X56597 CAA39935 XM_005258651 XP_005258708 XM_011526623 XP_011524925 XM_011548798 XP_011547100 XM_011548799 XP_011547101 NM_001436 NP_001427
Function S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). {ECO:0000269|PubMed:24352239}.
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:14583623, ECO:0000269|PubMed:2026646, ECO:0000269|PubMed:2414294, ECO:0000269|PubMed:24352239}. Note=Fibrillar region of the nucleolus.
Top Pathways Ribosome biogenesis in eukaryotes

Fibrillarin (C13C3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 14417 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C13C3
Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Fibrillarin (C13C3) Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 14612 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone C13C3
Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb (Alexa ® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with DyLight™ 488 Phalloidin #12935 (green). Red pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087 (fluorescent DNA dye).

Fibrillarin (C13C3) Rabbit mAb - 2639 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C13C3
Western blot analysis of extracts from various cell types using Fibrillarin (C13C3) Antibody.

Fibrillarin (T298) Antibody - 2147 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from MCF-7, Neuro2A, PC12 and COS cells, using Fibrillarin (T298) Antibody.

Fibrillarin (R122) Antibody (IF Specific) - 2159 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Confocal immunofluorescent analysis of COS cells using Fibrillarin (R122) Antibody (IF Specific) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Fibrillarin (G-4) - sc-166021 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fibrillarin (G-4): sc-166021. Western blot analysis of Fibrillarin expression in non-transfected: sc-117752 (A) and mouse Fibrillarin transfected: sc-126852 (B) 293T whole cell lysates.

Fibrillarin (G-8) - sc-374022 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fibrillarin (G-8): sc-374022. Western blot analysis of Fibrillarin expression in non-transfected: sc-117752 (A) and mouse Fibrillarin transfected: sc-126852 (B) 293T whole cell lysates.

Fibrillarin (H-9) - sc-514239 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fibrillarin (H-9): sc-514239. Western blot analysis of Fibrillarin expression in Hep G2 whole cell lysate.

Fibrillarin (D-14) - sc-11336 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Fibrillarin (D-14): sc-11336. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.

Fibrillarin (B-1) - sc-166001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fibrillarin (B-1): sc-166001. Western blot analysis of Fibrillarin expression in non-transfected: sc-117752 (A) and mouse Fibrillarin transfected: sc-126852 (B) 293T whole cell lysates and MOLT-4 nuclear extract (C).

Fibrillarin (N-15) - sc-11334 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Fibrillarin (F-6) - sc-166000 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Fibrillarin (F-6): sc-166000. Western blot analysis of Fibrillarin expression in non-transfected: sc-117752 (A) and mouse Fibrillarin transfected: sc-126852 (B) 293T whole cell lysates and MOLT-4 nuclear extract (C).

Fibrillarin (A-16) - sc-11335 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Fibrillarin (A-16): sc-11335. Western blot analysis of Fibrillarin expression in non-transfected: sc-117752 (A) and mouse Fibrillarin transfected: sc-126852 (B) 293T whole cell lysates.

Fibrillarin (H-140) - sc-25397 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Fibrillarin (H-140): sc-25397. Western blot analysis of Fibrillarin expression in Hep G2 (A) and C4 (B) whole cell lysates, MOLT-4 (C) and K-562 (D) nuclear extracts and rat liver tissue extract (E).

Anti-Fibrillarin antibody - ab137422 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Fibrillarin antibody (ab137422) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : MOLT4 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Fibrillarin antibody - ab153862 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Fibrillarin antibody (ab153862) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-Fibrillarin antibody - ab119396 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Fibrillarin antibody (ab119396) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : 293 cell extract

Anti-Fibrillarin antibody - ab76660 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Fibrillarin antibody (ab76660) at 1/50 dilution + K562 cell line lysates at 35 µg

Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker - ab154806 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10822(B)
All lanes : Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806) at 1/1000 dilutionLane 1 : HeLa lysateLane 2 : HepG2 lysateLane 3 : 293T lysateLane 4 : Molt4 lysateLysates/proteins at 10 µg per lane.

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker - ab166630 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10823(B)
ab166630 showing +ve staining in Human normal tonsil.

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Alexa Fluor® 488) - ab184817 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10823(B)
Overlay histogram showing HeLa cells stained with ab184817 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184817, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.This antibody gave a positive signal in HeLa fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (Alexa Fluor® 568) - ab202540 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10823(B)
ab202540 staining Fibrillarin in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202540 at 1/200 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (HRP) - ab196980 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR10823(B)
All lanes : Anti-Fibrillarin antibody [EPR10823(B)] - Nucleolar Marker (HRP) (ab196980) at 1/5000 dilutionLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab52256)Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-FBL / FIB / Fibrillarin Antibody - LS-C66042 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal

Anti-FBL / FIB / Fibrillarin Antibody - LS-C66043 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
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