TPR
| Gene Symbol | TPR |
|---|---|
| Entrez Gene | 7175 |
| Alt Symbol | - |
| Species | Human |
| Gene Type | protein-coding |
| Description | translocated promoter region, nuclear basket protein |
| Other Description | NPC-associated intranuclear protein|megator|nuclear pore complex-associated protein TPR|nucleoprotein TPR|translocated promoter region (to activated MET oncogene)|translocated promoter region protein|tumor potentiating region |
| Swissprots | Q15624 Q5SWY0 P12270 Q99968 |
| Accessions | CAA63904 EAW91203 EAW91204 EAW91205 EAW91206 P12270 AA688415 AK023111 AK309473 BC016514 AAH16514 BC025177 AAH25177 BC094764 AAH94764 BC156389 AAI56390 U69668 AAB48030 X63105 CAA44819 X66397 CAA47021 Y00672 CAA68681 XM_011509955 XP_011508257 NM_003292 NP_003283 |
| Function | Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope. Functions as a scaffolding element in the nuclear phase of the NPC essential for normal nucleocytoplasmic transport of proteins and mRNAs, plays a role in the establishment of nuclear-peripheral chromatin compartmentalization in interphase, and in the mitotic spindle checkpoint signaling during mitosis. Involved in the quality control and retention of unspliced mRNAs in the nucleus; in association with NUP153, regulates the nuclear export of unspliced mRNA species bearing constitutive transport element (CTE) in a NXF1- and KHDRBS1-independent manner. Negatively regulates both the association of CTE-containing mRNA with large polyribosomes and translation initiation. Does not play any role in Rev response element (RRE)-mediated export of unspliced mRNAs. Implicated in nuclear export of mRNAs transcribed from heat shock gene promoters; associates both with chromatin in the HSP |
| Subcellular Location | Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Nucleus envelope. Nucleus, nuclear pore complex. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Detected as discrete intranuclear foci with IFI204 (By similarity). In interphase, localizes to the nucleoplasmic side of the nuclear pore complex (NPC) core structure, forming a fibrous structure called the nuclear basket. Detected exclusively to the cytoplasmic margin of NPC (PubMed:7798308). Docking to the inner nucleoplasmic side of the NPC is mediated through binding to nucleoporins. Anchored by NUP153 to the NPC. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected as filaments that emanate from the nuclear basket of the NPC and extend to the nucleolus to delineate a chromatin-free network extending from the nucle |
| Tissue Specificity | Expressed in esophagus, ovary, liver, skin, smooth muscles, cerebrum and fetal cerebellum (at protein level). Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney. {ECO:0000269|PubMed:12424524, ECO:0000269|PubMed:9024684}. |
| Top Pathways | Thyroid cancer, RNA transport |
TPR CRISPR/Cas9 KO Plasmid (h) - sc-402928 from Santa Cruz Biotechnology
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TPR HDR Plasmid (h) - sc-402928-HDR from Santa Cruz Biotechnology
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TPR Double Nickase Plasmid (h) - sc-402928-NIC from Santa Cruz Biotechnology
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TPR Double Nickase Plasmid (h2) - sc-402928-NIC-2 from Santa Cruz Biotechnology
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TPR CRISPR Activation Plasmid (h) - sc-402928-ACT from Santa Cruz Biotechnology
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TPR CRISPR Activation Plasmid (h2) - sc-402928-ACT-2 from Santa Cruz Biotechnology
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TPR Lentiviral Activation Particles (h) - sc-402928-LAC from Santa Cruz Biotechnology
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TPR Lentiviral Activation Particles (h2) - sc-402928-LAC-2 from Santa Cruz Biotechnology
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TPR siRNA (h) - sc-45343 from Santa Cruz Biotechnology
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TPR shRNA Plasmid (h)-SH - sc-45343-SH from Santa Cruz Biotechnology
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TPR shRNA (h) Lentiviral Particles -PR - sc-45343-V from Santa Cruz Biotechnology
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TPR RNAi - H00007175-R01 from Novus Biologicals
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MISSION® esiRNA - EHU129911 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN21878 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND21878 from Sigma-Aldrich
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TPR siRNA (Human) - CRH4926 from Cohesion Biosciences
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TPR - MBS8230203 from MyBioSource
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shRNA set against Human Tpr (NM_003292.2) - SHH433440 from Creative biogene
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shRNA set against Human TPR(NM_003292.2) - SHL090184 from Creative biogene
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Human TPR siRNA - orb265428 from Biorbyt
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TPR shRNA Plasmid (Human) - CHH4926 from Cohesion Biosciences
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TPR miRNA 3'UTR clone - MiUTR1H-10679 from Creative biogene
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