USP16

Gene Symbol USP16
Entrez Gene 10600
Alt Symbol UBP-M, UBPM
Species Human
Gene Type protein-coding
Description ubiquitin specific peptidase 16
Other Description deubiquitinating enzyme 16|ubiquitin carboxyl-terminal hydrolase 16|ubiquitin specific protease 16|ubiquitin thioesterase 16|ubiquitin thiolesterase 16|ubiquitin-processing protease UBP-M|ubiquitin-specific processing protease 16|ubiquitin-specific-processing protease 16
Swissprots B4DFV8 Q9H3E6 B3KN13 Q53GP7 Q5VKN8 A8MU43 D3DSD9 B4DY37 Q53HA0 Q8NEL3 Q9Y5T5
Accessions CAB90432 EAX09927 EAX09928 EAX09929 Q9Y5T5 AF113219 AAG39290 AF126736 AAD20949 AK023247 BAG51175 AK025104 AK025989 AK090776 AK222681 BAD96401 AK222884 BAD96604 AK294284 BAG57569 AK300771 BAG62436 AK302247 BAG63599 AY333928 AAR13293 BC010375 BC030777 AAH30777 BQ637016 CB960929 DQ892395 ABM83321 DQ895610 ABM86536 W57837 NM_001001992 NP_001001992 NM_001032410 NP_001027582 NM_006447 NP_006438
Function Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T- lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. {ECO:0000255|HAMAP-Rule:MF_03062, ECO:0000269|PubMed:10077596, ECO:0000269|PubMed:17914355}.
Subcellular Location Nucleus {ECO:0000305|PubMed:17914355}.
Tissue Specificity Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. {ECO:0000269|PubMed:10077596}.

Ubp-M (B-3) - sc-390683 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ubp-M (B-3): sc-390683. Western blot analysis of Ubp-M expression in HeLa whole cell lysate.

Ubp-M (E-20) - sc-9766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Ubp-M (E-20): sc-9766. Western blot analysis of Ubp-M expression in K-562 (A), HeLa (B) and A-431 (C) whole cell lysates.

Ubp-M (N-20) - sc-9765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Ubp-M (H-57) - sc-366695 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ubp-M (H-57): sc-366695. Western blot analysis of Ubp-M expression in Hep G2 whole cell lysate.

Ubp-M (L-25) - sc-134140 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Ubp-M (L-25): sc-134140. Western blot analysis of Ubp-M expression in Hep G2 whole cell lysate.

Ubp-M (E-23) - sc-130906 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Ubp-M (E-23): sc-130906. Western blot analysis of Ubp-M expression in HL-60 whole cell lysate.

Anti-USP16 antibody - ab121650 from Abcam

Prices $443.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121650 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Anti-USP16 antibody - ab130319 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-USP16 antibody (ab130319) at 1/100 dilution + SK-BR-3 cell lysate at 35 µg

Anti-USP16 antibody [1B4] - ab156760 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B4
All lanes : Anti-USP16 antibody [1B4] (ab156760) at 1/200 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : SVT2 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.

Anti-USP16 antibody - N-terminal - ab189838 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-USP16 antibody - N-terminal (ab189838) at 1/500 dilutionLane 1 : COS7 cell extractLane 2 : Jurkat cell extractLane 3 : 293T cell extractLane 4 : NIH3T3 cell extractLane 5 : Mouse brain extractLane 6 : Mouse heart extractLane 7 : Mouse spleen extractLane 8 : Rat brain extract

Anti-USP16 antibody - N-terminal - ab135509 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human breast carcinoma tissue labelling USP16 with ab135509 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-USP16 Antibody (aa132-160, AP) - LS-C273174 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (aa132-160, APC) - LS-C273176 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (aa132-160, Azide-free, Biotin) - LS-C273177 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (aa132-160, FITC) - LS-C273178 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (aa132-160, PE) - LS-C273179 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (aa51-100) - LS-C80771 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 cell lysate. Antibody concentration: 2.5 ug/ml. Gel concentration: 12%.

Anti-USP16 Antibody (aa132-160, Azide-free, HRP) - LS-C273180 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (N-Terminus, AP) - LS-C284183 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (N-Terminus, APC) - LS-C284184 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (N-Terminus, Biotin) - LS-C284185 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (N-Terminus, Azide-free, HRP) - LS-C284186 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (N-Terminus, FITC) - LS-C284187 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (N-Terminus, PE) - LS-C284188 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-USP16 Antibody (aa386-416) - LS-C99559 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of anti-USP16 antibody in HL60 cell line lysate (35 ug/lane). USP16(arrow) was detected using the purified antibody.
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