TJP1

Gene Symbol TJP1
Entrez Gene 403752
Alt Symbol ZO-1, ZO1
Species Dog
Gene Type protein-coding
Description tight junction protein 1 (zona occludens 1)
Other Description tight junction protein ZO-1|zona occludens protein 1|zonula occludens 1|zonula occludens protein 1
Swissprots O97758
Accessions AAG43398 O97758 U55935 AAD11529 NM_001003140 NP_001003140
Function The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (By similarity). {ECO:0000250}.
Subcellular Location Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Cell junction, tight junction {ECO:0000250}. Cell junction {ECO:0000250}. Cell junction, gap junction {ECO:0000250}. Note=Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells (By similarity). {ECO:0000250}.
Top Pathways Adherens junction, Tight junction, Gap junction, Salmonella infection

Anti-ZO1 tight junction protein antibody - ab59720 from Abcam

Prices $391.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab59720 staining ZO1 in Caco-2 cells treated with (±)-palmitoylcarnitine chloride (ab141122), by ICC/IF. Membranar ZO1 expression loss correlates with increased concentration of (±)-palmitoylcarnitine chloride, as described in literature.The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab141122 ((±)-palmitoylcarnitine chloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab59720 (0.5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-ZO1 tight junction protein antibody - ab96587 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-ZO1 tight junction protein antibody (ab96587) at 1/3000 dilution (5% SDS PAGE) + H1299 whole cell lysate at 30 µg

Anti-ZO1 tight junction protein antibody - ab84934 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ZO1 tight junction protein antibody (ab84934) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ZO1 tight junction protein antibody [mAbcam 61357] - ab61357 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone mAbcam 61357
ZO1 tight junction protein was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab61357.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 240kDa, non specific band - 90kDa: We are unsure as to the identity of this extra band; ZO1 tight junction protein

Anti-ZO1 tight junction protein antibody - C-terminal - ab190085 from Abcam

Prices $378.00
Sizes 100 µl
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ZO1 tight junction protein with ab190085 at 10 µg/ml.

ZO-1 Polyclonal Antibody - 40-2300 from Thermo Fisher Scientific

Prices $373.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of mouse liver tissue using Rb anti-ZO-1 (N-term) (Cat. No. 40-2300). Image courtesy of James I. Nagy, PhD, University of Manitoba, Canada.

ZO-1 Polyclonal Antibody - 40-2200 from Thermo Fisher Scientific

Prices $373.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent staining of Caco-2 cells using Rb anti-ZO-1 (Mid) (Cat. no. 40-2200). Image courtesy of Jacey Bennis and Dr. James Anderson, University of North Carolina at Chapel Hill, NC.

ZO-1 Polyclonal Antibody - 61-7300 from Thermo Fisher Scientific

Prices $362.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Rabbit anti-ZO-1 (Z-R1) stained small intestine.

ZO-1 Monoclonal Antibody (ZO1-1A12), FITC - 33-9111 from Thermo Fisher Scientific

Prices $324.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ZO1-1A12
Immunofluorescence analysis of ZO-1 / TJP1 Antibody, FITC conjugate (ZO1-1A12) was done on 90% confluent log phase CaCo2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 / TJP1 Antibody, FITC conjugate (ZO1-1A12)(339111) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cell junctional localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.

ZO-1 Monoclonal Antibody (ZO1-1A12) - 33-9100 from Thermo Fisher Scientific

Prices $311.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone ZO1-1A12
Image courtesy of Dr. Alan S. Fanning, University of North Carolina at Chapel Hill, NC.
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